An Investigation of the Altered Textural Property in Woody Breast Myopathy Using an Integrative Omics Approach.

Woody breast (WB) is a myopathy observed in broiler (PM) characterized by its tough and rubbery texture with greater level of calcium content. The objective of this study was to investigate the functionality/integrity of WB sarcoplasmic reticulum (SR), which may contribute to the elevated calcium content observed in WB and other factors that may influence WB texture. Fourteen Ross line broiler PM [7 severe WB and 7 normal (N)] were selected, packaged, and frozen at -20°C at 8 h postmortem from a commercial processing plant.

The COSMIC Bubble Helmet: A Non-Invasive Positive Pressure Ventilation System for COVID-19.

COSMIC Medical, a Vancouver-based open-source volunteer initiative, has designed an accessible, affordable, and aerosol-confining non-invasive positive-pressure ventilator (NIPPV) device, known as the COSMIC Bubble Helmet (CBH). This device is intended for COVID-19 patients with mild-to-moderate acute respiratory distress syndrome. : CBH is composed of thermoplastic polyurethane, which creates a flexible neck seal and transparent hood.

Evaluation of skin-on goat meat processing on processing efficiency, carcass yield, meat quality, and sensory attributes.

The objective of this study was to evaluate the properties of skin-on and skin-off goat processing and carcasses for processing efficiency, fabrication time, biochemical factors, and sensory attributes for stewed goat meat. Thirty-one goats were harvested in 2 separate experiments, either skin-on (n = 16) or off (n = 15). The carcasses were fabricated into four primals and each primal was cut into cubes.

[Establishment and evaluation of loop-mediated isothermal amplification based on Plasmodium falciparum unique genes coding PHIST proteins].

Objective: To establish a novel convenient loop-mediated isothermal amplification (LAMP) method with the unique genes coding Plasmodium helical interspersed sub-telomeric superfamily (PHIST) for the rapid molecular diagnosis of P. falciparum.

Methods: The unique genes coding PHIST with high expression mRNA profile during the ring form or schizont period of P.

[Prokaryotic expression and identification of secretory proteins and peripheral membrane proteins of ].

Objective: To prokaryotic express and identify the recombinant plasmids containing the genes or gene segments which coding secreted or peripheral membrane protein of .

Methods: A number of 28 pET32 (+) previous constructed recombinant plasmids containing these genes or segments were transferred into BL21 (DE3) strain for culture via the CaCl transformation method, and then induced by IPTG for prokaryotic expression. Then, the bacteria were treated by broking the wall with ultrasonic, and the bacterial suspension, supernatant and precipitation were detected with 12% SDS-PAGE.