Correction: Dietary L-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model.

Correction for 'Dietary L-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model' by Mengdie Chen , , 2019, , 2390-2398, https://doi.org/10.1039/C9FO00123A.

Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model.

The intestinal epithelial layer forms a barrier through cell-cell tight junctions and breaking or even slightly disrupting this barrier can lead to serious pathological consequences, including infection and inflammation. Various amino acids have been shown to improve the intestinal tract, but the effect of tryptophan on the intestinal barrier has been controversial. Here, an in vitro Caco-2 cell model was built to investigate the protective and reparative effects of different concentrations of dietary l-Tryptophan (l-Trp) on lipopolysaccharide (LPS)-induced intestinal tight junction injury.

In vivo antitumor and antioxidative effects of a rapeseed meal protein hydrolysate on an S180 tumor-bearing murine model.

The antitumor and antioxidative activities of a rapeseed protein hydrolysate (RSCH) obtained from rapeseed meal were evaluated by using an in vivo S180 tumor-bearing Kunming mice model. Tumor-bearing female mice were given RSCH for 10 at doses of 0, 50, 100, and 150 mg/kg/d by gastric perfusion. RSCH significantly decreased the tumor weight by 44% and 53% in the 100 and 150 mg/kg/d groups, respectively, without causing mortality or growth retardation.

Effect of rapeseed peptide on DNA damage and apoptosis in Hela cells.

Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640 mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay.

Preparation and antioxidative properties of a rapeseed ( Brassica napus ) protein hydrolysate and three peptide fractions.

This study investigated the possibility of converting the insoluble rapeseed meal protein into functionally active ingredients for food applications. The rapeseed ( Brassica napus ) meal protein isolates were first digested by Alcalase and Flavourzyme, and the resultant rapeseed crude hydrolysate (RSCH) exhibited a dose-dependent reducing antioxidant power and hydroxyl radical scavenging ability. RSCH could also inhibit the malonyldialdehyde (MDA) generation by 50% in blood serum at 150 mg/mL.

Characterization and free radical scavenging activity of rapeseed meal polysaccharides WPS-1 and APS-2.

Two major polysaccharide fractions, WPS-1 and APS-2, were isolated from water-soluble and alkali-soluble extracts of Huaza No. 4 rapeseed meal with a stepwise procedure of D3520 macroporous adsorption resin column chromatography, ethanol precipitation, and DE-52 cellulose column chromatography. Physicochemical properties of the polysaccharides were determined by chemical methods, high -performance liquid chromatography (HPLC), gel permeation chromatography (GPC), and Fourier transform infrared spectrometry (FT-IR).

[Determination of lead in microemulsified rapeseed oil and bio-diesel oil by GFAAS].

Bio-diesel oil has attracted much attention as a substitutable energy sources for its renewable and eco-friendly property. However, problems of lead contamination in fuel are also emphasized increasingly at present. So it was of quite significance to determine the contents of lead in bio-diesel oil and its raw material rapeseed oil.

Characterization and immunomodulating activities of polysaccharide from Lentinus edodes.

The polysaccharide L-II was isolated and purified from the fruiting body of Lentinus edodes, which consisted of d-glucopyranose and had the molecular weight of 2.03 x 10(5) Da. We evaluated the effects of the polysaccharide L-II on the cellular immune response of Sarcoma 180-bearing mice.

[Anti-tumor effect of polysaccharide from Grifola frondosa and its influence on immunological function].

When the polysaccharide from Grifola frondosa (PGF) was taken orally by S180 bearing mice at the dosage of 50-150 mg/kg.d for 9 d, the inhibitory rate to S180 was 27.97%-38.