Effect of the transcription factor YY1 on the development of pancreatic endocrine and exocrine tumors: a narrative review.

Pancreatic tumors are classified into endocrine and exocrine types, and the clinical manifestations in patients are nonspecific. Most patients, especially those with pancreatic ductal adenocarcinoma (PDAC), have lost the opportunity to receive for the best treatment at the time of diagnosis. Although chemotherapy and radiotherapy have shown good therapeutic results in other tumors, their therapeutic effects on pancreatic tumors are minimal.

Roundabout homolog 1 inhibits proliferation via the YY1-ROBO1-CCNA2-CDK2 axis in human pancreatic cancer.

Pancreatic cancer (PC) is highly malignant and has a high mortality with a 5-year survival rate of less than 8%. As a member of the roundabout immunoglobulin superfamily of proteins, ROBO1 plays an important role in embryogenesis and organogenesis and also inhibits metastasis in PC. Our study was designed to explore whether ROBO1 has effects on the proliferation of PC and its specific mechanism.

[Surgical treatment of giant thymoma in a patient with myasthenia gravis].

We report a case report of giant thymoma (17 cm×14 cm×4 cm) in a 32-year-old male patient with myasthenia gravis. Examinations revealed that the tumor invaded the mediastinum, blood vessels and pericardium, and was histologically classified as WHO type B1 and Masaoka stage III. The patient developed myasthenia gravis crisis after radical resection of the thymoma and appropriate treatments with tracheotomy were administered.

Rapid Detection of Serratia fonticola by TaqMan Quantitative Real-Time PCR Using Primers Targeting the gyrB Gene.

A gyrB gene is present in the majority of bacterial species, and encodes the ATPase domain of DNA gyraseB-subunit protein, which is essential for transcription and replication of bacteria. The gyrB gene exhibits higher nucleotide sequence variability than the 16S rDNA gene and thus could be more reliable in differentiating Serratia fonticola. A species-specific primer pair and probe were designed for quantitative real-time PCR detection of S.

[Establishment of a rabbit model of small diameter vascular graft replacement].

Objective: To establish an rabbit model that mimics the hemodynamics of the bypass graft after coronary artery bypass surgery.

Methods: Sixteen New Zealand rabbits were randomly divided into two groups for abdominal aortic artery replacement using a 3-cm-long ePTFE graft with an inner diameter 4 mm through an incision at 1/3 from the middle to the lower part of the abdomen (group A) or in the lower abdomen (group B). The general conditions of the rabbits, operative time, number of collateral vessels that needed to be ligated, rate of massive intraoperative bleeding, fluctuation of vascular anastomosis after surgery, patency rate of the graft on day 7 after the operation were compared between the two groups.

[Brachiocephalic artery-sparing aortic arch repair combined with stent-graft elephant trunk technique for Stanford type A aortic dissection: analysis of 23 cases].

Objective: To assess the clinical outcomes of brachiocephalic artery-sparing aortic arch repair combined with stent-graft elephant trunk technique for treatment of Stanford type A aortic dissection.

Methods: Twenty-three patients with Stanford type A aortic dissection requiring arch replacement underwent brachiocephalic artery-sparing aortic arch repair combined with stent-graft elephant trunk technique. The operations were performed within 72 h (20 cases) or 3-14 days (3 cases) after the onset of aortic dissection.

Spontaneous regression of retinopathy of prematurity: incidence and predictive factors.

Aim: To evaluate the incidence of spontaneous regression of changes in the retina and vitreous in active stage of retinopathy of prematurity(ROP) and identify the possible relative factors during the regression.

Methods: This was a retrospective, hospital-based study. The study consisted of 39 premature infants with mild ROP showed spontaneous regression (Group A) and 17 with severe ROP who had been treated before naturally involuting (Group B) from August 2008 through May 2011.

Serum salusin-α levels are inversely correlated with the presence and severity of coronary artery disease.

We sought to measure serum salusin-α levels in patients with coronary artery disease (CAD) and to assess their correlation with the severity of the disease. We enrolled 172 patients with CAD and 91 controls. We assessed the angiographic severity of CAD by coronary atherosclerosis index (CAI) and detected serum salusin-α levels by enzyme-linked immunosorbent assay (ELISA).

[Thoraco laparoscopic esophagectomy versus open esophagectomy: a meta-analysis of outcomes].

Objective: To evaluate the outcomes of thoraco laparoscopic esophagectomy venus open esophagectomy for esophageal cancer.

Methods: Literature search was performed using PubMed, Embase, Cochrane Library, and Google Scholar databases, CBM, and CNKI from inception to July 2011 for comparative studies assessing thoraco laparoscopic esophagectomy and open esophagectomy. Data were extracted and evaluated by two reviewers independently according to the Cochrane Handbook for Systematic Reviews.

[Effect of inhaling specific phosphodiesterase inhibitor on lung injury induced by cardiopulmonary bypass].

Objective: To evaluate the effect of pretreatment by inhaling specific phosphodiesterase inhibitor on lung injury induced by cardiopulmonary bypass (CPB).

Methods: From April 2010 to November 2010, 30 patients were divided randomly into two groups: control group (n = 15) and milrinone group (n = 15). In milrinone group, 5 mg milrinone diluted by 5ml normal saline was inhaled per 8 h two days pre-operation.

[Quality of life in patients with esophageal carcinoma undergoing thoracoscopic and laparoscopic esophagectomy and circular stapled cervical esophagogastric anastomosis via retrosternal route].

Objective: To evaluate the quality of life (QOL) in patients with esophageal carcinoma after thoracoscopic and laparoscopic esophagectomy and circular stapled cervical esophagogastric anastomosis via retrosternal route or three-incision open surgery.

Methods: A total of 63 patients with middle-upper esophageal carcinoma who underwent radical surgical resection from January 2009 to October 2010 were enrolled in this study. Thirty-three patients underwent combined laparoscopic and thoracoscopic surgery and 30 three-incision open surgery.

[Therapeutic effect of tracheal anastomosis versus interventional bronchoscopy in the treatment of airway stenosis].

Objective: To compare the therapeutic effects of tracheal anastomosis and interventional bronchoscopy in the treatment of airway stenosis.

Methods: From February, 1990 to February 2008, 53 patients with airway stenosis including 32 male and 21 female patients aged 2 to 80 years (mean 38 years) received end-to-end anastomosis or bronchoscopic stent-graft treatment in our hospital. End-to-end anastomosis of the narrow sleeves resected was performed in 26 cases, balloon dilatation in 14 cases, and stent implantation in 13 cases.

[Clinical analysis of primary thymic lymphoma: a report of 27 cases].

Objective: To investigate clinicopathologic features and optimal treatment of primary thymic lymphoma.

Methods: Clinical records of 27 primary thymic lymphoma patients treated from 1990 to 2007 were reviewed.

Results: Of the 27 patients, 8 received mastectomy and chemotherapy, 12 received excision of the thymic lesion and chemotherapy, 5 received chemotherapy alone, and 2 received lesion excision alone.

[Validation of the non-cellular metastasis hypothesis of malignant tumors in mice].

Objective: To test the hypothesis that malignant tumor metastasis is mediated also through a non-cellular, essentially molecular, mechanism in addition to the cellular pathway.

Methods: The sex-determining region on the Y chromosome was detected as the marker of the primary tumors using PCR in Lewis lung carcinoma (LLC) in vitro and in female C57BL/6 mice bearing LLC with spontaneous metastasis. The macroscopic and microscopic metastases in the tumor-bearing mice were examined for SRY expression by PCR and in situ hybridization, using the tissues from male and female mice as the positive or negative controls.

[Preparation and dose-effect analysis of ecdysterone cream for promoting wound healing].

Objective: To prepare ecdysterone cream for promoting wound healing and conduct the dose-effect analysis to determine the optimal concentration.

Methods: The cream substrate containing 4 concentrations (0.625%, 1.

[Design and experimental application of a device for direct vision intracardiac operation without cardiopulmonary bypass].

Objective: To design a device for direct vision intracardiac operation without cardiopulmonary bypass, and assess its applicability preliminarily.

Methods: The device was designed according to the clinical needs of intracardiac operation and used in operations for repairing atrial septal defect in 5 ex vivo porcine heart models. The practical applicability of this device was thoroughly tested and the results of the operations were evaluated.

[L-arginine pretreatment lowers cerebral metabolism for cerebral protection during deep hypothermic circulatory arrest].

Objective: To evaluate the effect of L-arginine pretreatment on cerebral metabolism for cerebral protection during deep hypothermic circulatory arrest (DHCA).

Methods: Fifteen healthy adult canines of either sex weighing 14.7-/+2.

[Screening of differentially expressed proteins from human esophageal cancer and esophageal tissues by two-dimensional difference gel electrophoresis and mass spectrometry].

Objective: To establish two-dimensional electrophoresis profiles from human esophageal cancer tissue and paired normal esophageal tissue and identify differentially expressed proteins to identify the molecular markers for early-stage diagnosis.

Methods: The total proteins of human esophageal cancer tissue and paired normal esophageal tissue were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). The 6 differentially expressed proteins were analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).

[Evaluation of ultramicroporous expanded polytetrafluoroethylene mitral valve under pulsatile flow condition in vitro].

Objective: To test 25# and 27# ultramicroporous expanded polytetrafluo-roethylene mitral valve (UPMV) under pulsatile flow condition in vitro.

Methods: Six 25# and six 27#UPMV were tested using TH-1200 HV Prosthesis Pulsatile Flow Tester in vitro at the cycling rate of 70 cycle/min, with the systolic pressure maintained at about 16 kPa (120 mmHg), diastolic pressure at 10.7 kPa (80 mmHg), and the percentage of mean forward flow at 35%.

[Ecdysterone promotes wound healing in rabbits].

Objective: To study the efficacy of ecdysterone in promoting wound healing in rabbits.

Methods: Twenty-one New Zealand rabbits were randomized equally into 3 groups and in each rabbit, 4 separate full-thickness skin wounds were induced and treated with dexamethasone, ecdysterone and Yunnan white powder, or untreated, respectively. On day 3, 6, and 9, respectively, one group of rabbits were sacrificed and the local wound tissue was obtained for pathological examination.

[Accelerated fatigue test in vitro of expanded polytetrafluoroethylene artificial heart valve].

Objective: To study the durability of expanded polytetrafluoroethylene artificial heart valve (ePTFE AHV).

Methods: Six ePTFE AHVs were tested for 400 million times against accelerated fatigue using TH-2200 artificial heart valve exosomatic accelerated fatigue instrument. Hydromechanical parameters of fore-and-aft accelerated fatigue test of the 6 AHVs were obtained by TH-1200 artificial heart valve exosomatic pulsatile stream instrument.

[Comparative study of video-assisted thoracoscopic surgery vs thoracic tube drainage in synthetic therapy for malignant pleural effusion secondary to non-small cell lung cancer].

Objective: To study the value of video-assisted thoracoscopic surgery (VATS) and identify its indications in synthetic therapy for malignant pleural effusion secondary to non-small cell lung cancer.

Methods: A prospective randomized single-blinded controlled clinical trial was conducted. Fifty-three patients with moderate or large amount of ipsilateral malignant pleural effusion (MPE) secondary to non-small cell lung cancer (NSCLC) were randomly divided into VATS group and tube drainage group (TD group).

[Experimental study of cerebral protection by retrograde vs selective antegrade cerebral perfusion during deep hypothermic circulatory arrest].

Objective: To compare the effect of cerebral protection between retrograde cerebral perfusion (RCP) and selective antegrade cerebral perfusion (SACP) during deep hypothermic circulatory arrest (DHCA) in canine models.

Methods: Fifteen healthy adult dogs were randomly divided into 3 groups (n=5), namely the simple DHCA group (group I), DHCA+RCP group (group II) and DHCA+SACP group (group III). Extrocorporeal circulatory was established routinely in the dogs, and DHCA commenced when the nasopharyngeal temperature was reduced to 18 degrees C.

[The protective effects to the function of kidney and long by clearing of cytokines in patients with open-heart surgery].

Objective: To observe the effect of cytokines absorption on renal and respiratory function in patients with open-heart surgery.

Methods: 30 patients undergoing valve replacement with cardiopulmonary bypass (CPB) were randomly divided into two groups. A sulfonated polyacrylonitrile hemofilter (AN69) that has been used to absorb cytokines was connected into the efferent limb of CPB in Group A (n = 15), and a cellulose triacetate hemofilter (CT 190G) instead of AN69 was used as controls (Group B, n = 15).