Development of a TaqMan qPCR for the Simultaneous Detection of the TuMV and BBWV2 Viruses Responsible for the Viral Disease in .

(Miq.) Pax, a highly valued Chinese medicinal plant, is experiencing a notable decline in yield and quality due to viral diseases, particularly caused those by TuMV and BBWV2. Currently, the absence of a quantitative detection method for these viruses in impedes the accurate diagnosis.

TRIOL attenuates intracerebral hemorrhage injury by bidirectionally modulating microglia- and neuron-mediated hematoma clearance.

Intracerebral hemorrhage (ICH) represents the most severe subtype of stroke, and the lack of effective clinical pharmacotherapies poses a substantial threat to human health. Hematoma plays a crucial role in determining the prognosis of ICH patients by causing primary mechanical extrusion, followed by secondary brain injuries, such as cerebral edema, iron-mediated oxidative stress, and inflammation resulting from its degradation products. 5α-androst-3β,5α,6β-triol (TRIOL) is a neuroprotective steroid currently undergoing phase II clinical trial for acute ischemic stroke with anti-oxidative and anti-inflammatory properties.

CircRNA-loaded DC vaccine in combination with low-dose gemcitabine induced potent anti-tumor immunity in pancreatic cancer model.

Although promising, dendritic cell (DC) vaccines may not suffice to fully inhibit tumor progression alone, mainly due to the short expression time of the antigen in DC vaccines, immunosuppressive tumor microenvironment, and tumor antigenic modulation. Overcoming the limitations of DC vaccines is expected to further enhance their anti-tumor effects. In this study, we constructed a circRNA-loaded DC vaccine utilizing the inherent stability of circular RNA to enhance the expression level and duration of the antigen within the DC vaccine.

Restorative Effects of Short-Chain Fatty Acids on Corneal Homeostasis Disrupted by Antibiotic-Induced Gut Dysbiosis.

The gut microbiota plays a crucial regulatory role in various physiological processes, yet its impact on corneal homeostasis remains insufficiently understood. Here, we investigate the effects of antibiotic-induced gut dysbiosis (AIGD) and germ-free (GF) conditions on circadian gene expression, barrier integrity, nerve density, and immune cell activity in the corneas of mice. Through RNA sequencing, we found that both AIGD and GF conditions significantly disrupted the overall transcriptomic profile and circadian transcriptomic oscillations in the cornea.

Seasonal instability of phytoplankton community in extreme arid lake basin during interval period of large-scale ecological water transport.

Large-scale water transport helps solve the imbalance in water resources. Studies on ecological benefits after long-term EWT mainly focus on vegetation restoration and increase in water surface. However, the maintenance of aquatic communities in the EWT context is a major challenge.

Alleviation of lipopolysaccharide-induced heart inflammation in poultry treated with carnosic acid via the NF-κB and MAPK pathways.

In intensive poultry farming, environmental stress, pathogen infections, and noise can negatively impact growth or cause sudden death, leading to economic losses. The prevalent use of antibiotics as feed additives to prevent diseases in broilers has raised concerns about antibiotic resistance and highlighted the need for safer and more effective alternatives. Carnosic acid (CA), a bioactive compound derived from rosemary, exhibits notable pharmacological properties, including anti-inflammatory and antioxidant effects.

The host restriction factor SERINC5 inhibits HIV-1 transcription by negatively regulating NF-κB signaling.

Serine incorporator 5 (SER5) can be incorporated into HIV-1 virions to block viral entry by disrupting the envelope glycoprotein-mediated viral fusion to the plasma membrane. Recent studies suggest that SER5 also inhibits HIV-1 mRNA transcription and the subsequent progeny virion biogenesis. However, the underlying mechanisms through which SER5 antagonizes the viral transcription remain poorly understood.

Nanoparticle-Mediated Gene Delivery for Bone Tissue Engineering.

Critical-sized bone defects represent an urgent clinical problem, necessitating innovative treatment approaches. Gene-activated grafts for bone tissue engineering have emerged as a promising solution. However, traditional gene delivery methods are constrained by limited osteogenic efficacy and safety concerns.

The first complete chloroplast genome of (Forssk.) Boiss. 1882 (Cymodoceaceae).

(Forssk.) Boiss. 1882 is a seagrass species.

Evaluation of bacterial anti-adhesion and sustained antimicrobial abilities of chlorhexidine gluconate-loaded phase-transited lysozyme nanoparticle suspension on dentine: An ex vivo study.

Aim: To evaluate the effect of chlorhexidine gluconate-loaded phase-transited lysozyme (CHG@PTL) coating on inhibiting bacterial adhesion and biofilm formation in an ex vivo root canal dentine model.

Methodology: The physicochemical and structural characteristics of CHG@PTL nanoparticle suspension and its coating formed on the dentine surface were analysed by thioflavin T fluorescence assay, transmission electron microscopy and confocal laser scanning microscopy (CLSM). The sustained chlorhexidine release profile of the CHG@PTL coating on the dentine surface was compared with that of the 2% CHG solution.

Linkage between soil stoichiometric imbalance and microbial carbon use efficiency in desert steppe under different grazing intensities.

Long-term grazing alters soil resource availability and microbial biomass stoichiometry in grassland ecosystems. Exploring the relationship between soil stoichiometric imbalance and microbial carbon use efficiency (CUE) in grazed desert steppe can help understand soil carbon dynamics from a microbial perspective. Based on a long-term grazing platform in desert steppe of Inner Mongolia established in 2004, taking heavy, moderate and light grazing intensities, using no grazing as a control, we measured soil available nutrients, microbial biomass and associated enzyme activities for their acquisition, and calculated soil microbial CUE using ecological stoichiometry.

The effectiveness of stage-matched interventions in changing waste source separation behaviour: Evidence from a consecutive quasi-experiment.

In many developing countries, inefficient waste source separation poses a significant challenge to sustainable waste management systems, hindering progress towards a circular economy. Previous research has shown mixed results regarding the effectiveness of informational interventions and has not thoroughly evaluated regulatory measures. This study innovatively employs a quasi-experimental design, enhanced by successive surveys, to assess the impact of targeted interventions on behavioural changes in waste separation practices.

Analysis of dynamic levitation process of the particle chain in a nonlinear standing wave field.

This research delves into the dynamic behavior of acoustic levitation of the particle chain in a nonlinear standing wave field. Experimental acoustic levitation control tests reveal bifurcation and jump phenomena during dynamic adjustments to resonant cavity height. Employing the 10-particle chain experiments and the COMSOL simulation models, the Sine-Gordon 2D vibration model is established to study the dynamic deformation process of the particle chain.

Linking proteomic function and structure to electroactive biofilms development across electrode orientations.

The functionality of electroactive biofilms (EABs) is profoundly influenced by the proteomic dynamics within microbial communities, particularly through the participation of proteins in electron transfer. This study explored the impact of electrode surface orientation, measured by varying oblique angles, on the performance of EABs in bioelectrochemical systems (BES). Utilizing quantitative proteomics, results indicated that a slightly oblique angle (45°) optimized the spatial arrangement of microbial cells, enhancing electron transport efficiency compared to other angles tested.

Experimental research on the horizontally getting together behaviors of acoustically manipulated bi-particle.

This study aims at the controllability of acoustically manipulated non-contact aggregation of bi-particle. Based on the acoustic wave radiation model of the transducer and the acoustic wave interference theory, the mathematical model between the spatial levitation point and the phase of the phased array ultrasonic array unit is established. A double-sided phased array test system was developed and the phase calculation algorithm for independently manipulating bi-particle was optimized.