Drug Screening of Flavonoids as Potential VEGF Inhibitors Through Computational Docking and Cell Models.

Vascular endothelial growth factor (VEGF), also known as VEGF-A, has been linked to various diseases, such as wet age-related macular degeneration (wAMD) and cancer. Even though there are VEGF inhibitors that are currently commercially available in clinical applications, severe adverse effects have been associated with these treatments. There is still a need to develop novel VEGF-based therapeutics against these VEGF-related diseases.

Advances in the Recovery of Critical Rare Dispersed Metals (Gallium, Germanium, Indium) from Urban Mineral Resources.

Urban mineral resources, with their significant recycling potential, have increasingly accumulated worldwide and become an important source for extracting valuable metals, particularly critical rare dispersed metals (CRDMs) such as gallium, germanium, and indium. As the electronics industry continues to grow rapidly, the demand for CRDMs is rising. However, CRDMs in primary mineral resources are often found in small, dispersed concentrations, making extraction challenging.

Characterization and Nutritional Intervention Effects of Type 5 Resistant Starch in Hyperlipidemia Mice.

Numerous reports have indicated that the type 3 resistant starch (RS3) derived from can regulate lipid metabolism. However, it remains unclear whether the type 5 resistant starch (RS5) exhibits similar effects. In this study, RS5 was prepared from native starch and lauric acid through a hydrothermal method for the first time, and its nutritional intervention effects on hyperlipidemia in mice were investigated.

Near-infrared fluorogenic RNA for in vivo imaging and sensing.

Fluorogenic RNA aptamers have various applications, including use as fluorescent tags for imaging RNA trafficking and as indicators of RNA-based sensors that exhibit fluorescence upon binding small-molecule fluorophores in living cells. Current fluorogenic RNA:fluorophore complexes typically emit visible fluorescence. However, it is challenging to develop fluorogenic RNA with near-infrared (NIR) fluorescence for in vivo imaging and sensing studies.

Lingo1 in the hippocampus contributes to cognitive dysfunction after anesthesia and surgery in aged mice.

Cognitive impairment caused by anesthesia and surgery is one of the most common complications with multiple etiologies that occurs in elderly patients. The underlying mechanisms are not fully understood, and there is a lack of therapeutic strategies. Increasing evidence has demonstrated that myelin loss, abnormal phosphorylation of the tau protein and neuronal apoptosis are substantial driving factors of cognitive deficits.

Rapid Determination of Crocin-I in Gardenia Fruits (Gardenia jasminoides Ellis) by Combining Spectral and Image Data Through Hyperspectral Imaging.

Introduction: Crocin-I, a water-soluble carotenoid pigment, is an important coloring constituent in gardenia fruit. It has wide application in various industries such as food, medicine, chemical industry, and so on. So the content of crocin-I plays a key role in evaluating the quality of gardenia.

Microbial degradation mechanisms, degradation pathways, and genetic engineering for pyrethroids: current knowledge and future perspectives.

Pyrethroids are synthetic products derived from natural pyrethroids present in flowers and are extensively used as pesticides for agriculture, animal husbandry, and household pest control. However, excessive and prolonged usage of pyrethroid insecticides can result in adverse effects on both non-target and target species. Therefore, effective technologies need to be developed to remove pyrethroid contamination and ensure environmental safety.

Corrigendum: Bruceine H mediates EGFR-TKI drug persistence in NSCLC by Notch3-dependent β-catenin activating FOXO3a signaling.

[This corrects the article DOI: 10.3389/fonc.2022.

Phenotypic Profiling of Tigecycline-resistant Klebsiella pneumoniae Strains Induced In vitro.

Tigecycline is one of the last-resort treatment options for infections caused by carbapenem-resistant Klebsiella pneumoniae (KP). Unfortunately, tigecycline resistance is increasingly reported and causes an unprecedented public health crisis worldwide. Although studies on tigecycline resistance are expanding, the underlying mechanisms are not fully understood.

Anti-inflammatory and heat shock protein-inhibiting nanoplatform for synergetic cancer chemo/photothermal therapy.

Photothermal therapy is a novel and promising method for cancer treatment due to its controllable property, noninvasive nature, and high selectivity. Nevertheless, tumor recurrence of inflammatory response and tumor tolerance of heat shock protein over-expression remain serious challenges in current photothermal therapy. Additionally, the high dosage requirement of nanomaterial for optimal imaging and therapeutic effect would result in various side effects, organ excretion burdens, and long-term accumulation in the body.

Coordination Induced Spin State Transition Switches the Reactivity of Nickel (II) Porphyrin in Hydrogen Evolution Reaction.

Electron spin plays a critical role in chemical processes, particularly in reactions involving metal complexes with unpaired electrons. However, more definitive state-to-state experiments are needed to better elucidate the role of electronic spin. Herein, we chose nickel (II) 5,10,15,20-tetrakis(pentafluorophenyl) porphyrin 1 as a catalyst, which allows switching from a low spin to a high spin state of Ni (II) center through an axial pyridine coordination, for electrocatalytic hydrogen evolution reaction (HER).

2-Aminobenzothiazole based adjuvant of polymyxin E against Gram-negative bacteria.

Antibiotic resistance has emerged as a pressing global health issue. Polymyxin E, commonly regarded as a clinically important antibiotic, faces limitations due to its dose-dependent toxicity. A crucial strategy to combat this is the development of an antibiotic adjuvant to enhance efficacy while reducing dosage.

Live-Cell Imaging of mRNA Using a Pepper RNA Tag.

Live-cell imaging of mRNA enables tracking of mRNA localization and its dynamics in real time. This is fundamentally important in understanding how cells use RNA to regulate gene expression and orchestrate biological processes. Here, we describe a method of using an engineered RNA tag, called Pepper RNA tag, to visualizing mRNA in living cells.

Rapid on-site detection of illicit drugs in urine using C18 pipette-tip based solid-phase extraction coupled with a miniaturized mass spectrometer.

Drug abuse is a social issue worldwide, and there is an increasing demand for on-site rapid detection of illicit drugs. In this study, a rapid and simple analytical method for the detection of 6-monoacetylmorphine (6-MAM), methamphetamine (MA), methylenedioxymethamphetamine (MDMA), ketamine (K), norketamine (NK), and cocaine (COC) in urine was developed. The developed method combines C18 pipette-tip based solid-phase extraction (C18 PT-SPE) with a miniaturized mass spectrometer (miniMS), exhibiting remarkable simplicity, high sensitivity, and strong reliability, compared with the conventional method.

Poly-γ-glutamic production by solid-state fermentation of Bacillus natto in ammonia nitrogen movement and soil water retention processes.

A high polyglutamic acid (γ-PGA) producing strain of Bacillus natto UV-40-50 was screened by ultraviolet mutagenesis treatment and identified as still belonging to the Bacillus specie. The optimal fermentation medium composition for solid state fermentation (SSF) of B. natto strain UV-40-50 strain was determined by one-way analysis of variance, under which the yield of γ-PGA was 55.

The activation of inflammatory responses in the brain is potentiated by over-expressing acetylcholinesterase in myeloid lineage of transgenic mice.

Acetylcholinesterase (AChE) has functions in neuroinflammation, beyond its classical role in neurotransmission. Understanding the role of AChE in neuroinflammation is of great significance, as it highlights the potential therapeutic targets for the treatment of neurodegenerative diseases. In an in vitro study, the expression of AChE was up-regulated in lipopolysaccharide (LPS)-induced microglia/macrophage and contrarily potentiated the inflammatory responses via disturbing the cholinergic anti-inflammatory pathway (CAP).

Copper-doped orange peel biochar activated peroxydisulfate for efficient degrading tetracycline: The critical role of C-OH and Cu.

The usage of biomass waste has aroused great concern in water purification. In this study, copper-doped orange peel biochar (Cu-OPB) prepared by one-step carbonization was firstly used to activate peroxydisulfate (PDS) to eliminate tetracycline (TC). The optimized Cu-OPB showed great activation ability for PDS and could degrade 93.

Cholinergic Signaling Mediated by Muscarinic Receptors Triggers the Ultraviolet-Induced Release of Melanosome in Cultured Melanoma Cells.

In skin, melanin is synthesized and stored in melanosomes. In epidermal melanocytes, melanosomes are transported to and internalized by the neighboring keratinocytes, subsequently leading to skin pigmentation. Ultraviolet (UV) radiation induces the release of acetylcholine (ACh) from keratinocytes, which in turn activates ACh receptors (AChRs) on nearby melanocytes, forming a proposed "skin synapse".

Chloroplast elongation factors break the growth-immunity trade-off by simultaneously promoting yield and defence.

Chloroplasts regulate plant development and immunity. Here we report that potato chloroplast elongation factors StTuA and StTuB, targeted by Phytophthora infestans RXLR effector Pi22926, positively regulate immunity and growth. Plants expressing Pi22926, or silenced for TuA/B, show increased P.

Voltage abnormity prediction method of lithium-ion energy storage power station using informer based on Bayesian optimization.

Accurately detecting voltage faults is essential for ensuring the safe and stable operation of energy storage power station systems. To swiftly identify operational faults in energy storage batteries, this study introduces a voltage anomaly prediction method based on a Bayesian optimized (BO)-Informer neural network. Firstly, the temporal characteristics and actual data collected by the battery management system (BMS) are considered to establish a long-term operational dataset for the energy storage station.

Protocol for a randomized controlled trial of intensive blood pressure control on cardiovascular risk reduction in patients with atrial fibrillation: Rationale and design of the CRAFT trial.

Background: Co-morbid hypertension is strong predictor of adverse cardiovascular (CV) outcomes in patients with atrial fibrillation (AF) but the optimal target for blood pressure (BP) control in this patient population has not been clearly defined.

Methods: The Cardiovascular Risk reduction in patients with Atrial Fibrillation Trial (CRAFT) is an investigator-initiated and conducted, international, multicenter, open-label, parallel-group, blinded outcome assessed, randomized controlled trial of intensive BP control in patients with AF. The aim is to determine whether intensive BP control (target home systolic blood pressure [SBP] <120 mmHg) is superior to standard BP control (home SBP <135 mmHg) on the hierarchical composite outcome of time to CV death, number of stroke events, time to the first stroke, number of myocardial infarction (MI) events, time to the first MI, number of heart failure hospitalization (HFH) events, and time to the first HFH.

Inhibition of jarosite heterogeneous crystallization on anglesite via in-situ formation of competitive substrate.

Heterogeneous crystallization is a common occurrence during the formation of solid wastes. It leads to the encapsulation of valuable/hazardous metals within the primary phase, presenting significant challenges for waste treatment and metal recovery. Herein, we proposed a novel method involving the in-situ formation of a competitive substrate during the precipitation of jarosite waste, which is an essential process for removing iron in zinc hydrometallurgy.