Serological evidence of sarbecovirus exposure along Sunda pangolin trafficking pathways.

Background: Early in the coronavirus disease 2019 (COVID-19) pandemic, Sunda pangolins (Manis javanica) involved in the illegal wildlife trade in mainland China were identified as hosts of severe acute respiratory syndrome-related coronaviruses (SARSr-CoVs). Although it is unconfirmed whether pangolins or other traded wildlife served as intermediate hosts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the trafficking of pangolins presents a clear risk for transmission of viruses with zoonotic and epizootic potential regardless. We have investigated the origins of pangolin carcasses seized in Hong Kong and have evaluated their potential exposure to SARSr-CoVs, other coronaviruses, and paramyxoviruses, aiming to address a gap in our knowledge with regard to the role of wildlife trade in the maintenance and emergence of pathogens with zoonotic and epizootic potential.

Surveillance of Rodent Pests for SARS-CoV-2 and Other Coronaviruses, Hong Kong.

We report surveillance conducted in 217 pestiferous rodents in Hong Kong for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We did not detect SARS-CoV-2 RNA but identified 1 seropositive rodent, suggesting exposure to a virus antigenically similar to SARS-CoV-2. Potential exposure of urban rodents to SARS-CoV-2 cannot be ruled out.

Complete mitochondrial genomes of ancient canids suggest a European origin of domestic dogs.

The geographic and temporal origins of the domestic dog remain controversial, as genetic data suggest a domestication process in East Asia beginning 15,000 years ago, whereas the oldest doglike fossils are found in Europe and Siberia and date to >30,000 years ago. We analyzed the mitochondrial genomes of 18 prehistoric canids from Eurasia and the New World, along with a comprehensive panel of modern dogs and wolves. The mitochondrial genomes of all modern dogs are phylogenetically most closely related to either ancient or modern canids of Europe.

Influence of manual therapy on functional mobility after joint injury in a rat model.

Context: Animal models can be used to investigate manual therapy mechanisms, but testing manipulation in animal models is problematic because animals cannot directly report their pain.

Objective: To develop a rat model of inflammatory joint injury to test the efficacy of manual therapy in reducing nociception and restoring function.

Methods: The authors induced acute inflammatory joint injury in rats by injecting carrageenan into the ankle and then measured voluntary running wheel activity in treated and untreated rats.

Quantification of blood velocity and flow rates in the uterine vessels using echo planar imaging at 0.5 Tesla.

Purpose: To measure uterine artery and vein blood velocity and flow rate profiles using MRI during normal pregnancy.

Materials And Methods: A two-shot velocity magnitude-encoded echo planar imaging (EPI) sequence is used at a magnetic field 0.5T.

[The development of the life evaluation questionnaire for hemodialysis patients].

Objective: Existing quality of life measures fail to incorporate patients' own understanding of evaluation of life following the diagnosis of end stage renal failure (ESRF). Previous qualitative research has identified ways of evaluating life that have not been captured by existing quantitative research and that can be possible targets for clinical practice. Nonetheless, quantification is necessary if clinicians are to be informed of potentially important ways of evaluating life.

[Hemodialysis patients' evaluation of their lives].

Objective: The experience of end-stage renal failure (ESRF) should not be understood merely in terms of functional impairment and increases in emotional distress. It should be assessed in its own right. Qualitative research is necessary for describing patients' own experience of ESRF so that findings are not influenced by psychological theory.

Haemodialysis patients' beliefs about renal failure and its treatment.

Patients' beliefs about illness are important because they influence adherence and adjustment, but they are often surprising and idiosyncratic. Qualitative research can identify them in ways that are not shaped by psychological theory, but quantification is necessary if clinicians are to be informed about the beliefs that are likely to be prevalent in their patients. Qualitative analysis of interviews with 16 haemodialysis (HD) patients identified beliefs about end-stage renal failure (ESRF) and its treatment that were formed into a questionnaire, completed by 156 similar patients.

Correction of spatial distortion in EPI due to inhomogeneous static magnetic fields using the reversed gradient method.

Purpose: To derive and implement a method for correcting spatial distortion caused by in vivo inhomogeneous static magnetic fields in echo-planar imaging (EPI).

Materials And Methods: The reversed gradient method, which was initially devised to correct distortion in images generated by spin-warp MRI, was adapted to correct distortion in EP images. This method provides point-by-point correction of distortion throughout the image.

The nurse's role in patient-centred medicines management.

Nurses in all care settings are crucial to the effective delivery of medicines management. This paper argues that adopting a collaborative approach will help patients get the most out of their medication and reduce non-compliance. The 'concordance' approach recognises partnership rather than directorship, and is based on arriving at shared agreements.

In utero perfusing fraction maps in normal and growth restricted pregnancy measured using IVIM echo-planar MRI.

The aim of this study was to measure and portray blood movement in the placenta in vivo in normal and growth restricted pregnancies, using Intra Voxel Incoherent Motion (IVIM) magnetic resonance imaging. Thirteen patients with apparently normal healthy pregnancies were scanned at 31+/-7 (mean+/-s.d.

In vivo intravoxel incoherent motion measurements in the human placenta using echo-planar imaging at 0.5 T.

This paper presents the first in vivo measurements of intravoxel incoherent motion in the human placenta, obtained using the pulsed gradient spin echo (PGSE) sequence. The aims of this study were two-fold. The first was to provide an initial estimate of the values of the IVIM parameters in this organ, which are currently unknown.

In vivo perfusion measurements in the human placenta using echo planar imaging at 0.5 T.

This paper presents the first in vivo measurements of perfusion in the human placenta from 20 weeks gestational age until term, using the non-selective/selective inversion recovery echo-planar imaging sequence, in which data is alternately acquired following a selective and non-selective inversion pulse. Twenty pairs of images were collected, two each at the following inversion times: 20, 310, 610, 910, 1110, 1410, 1910, 2810, 3310, and 4510 ms with the sequence being repeated with a repetition time (TR) of 10 s. The results of these measurements were used to suggest the optimum sequence for future work in terms of the signal to noise ratio in the measured perfusion rate in a given measurement time.

In vivo relaxation time measurements in the human placenta using echo planar imaging at 0.5 T.

This paper presents the first in vivo measurements of the nuclear magnetic resonance relaxation times T1 and T2 at 0.5 T in the human placenta from 20 weeks gestational age until term, in both normal and compromised pregnancies. T1 measurements were performed by using both an inversion recovery sequence and the Look-Locher echo planar imaging (EPI) sequence on a total of 41 women with normal pregnancies and 11 women with compromised pregnancies.

Demonstration of changes in fetal liver erythropoiesis using echo-planar magnetic resonance imaging.

This study investigated the variation in magnetic resonance characteristics of the fetal liver during a time of changing erythropoietic function. Echo-planar imaging was carried out in 25 normal pregnant women at 20 and 26 wk gestation. The signal intensity from regions of the fetal liver, background image, and maternal back muscle and the highest signal intensity from the maternal spinal cord were measured and compared with the signal intensity of amniotic fluid.

Neuro-endoscopic third ventriculostomy: evaluation with magnetic resonance imaging.

We have performed a prospective study of the use of magnetic resonance (MR) imaging in 14 patients undergoing neuro-endoscopic third ventriculostomy. MR imaging was undertaken prior to the endoscopy and serial studies were carried out after the procedure. MR imaging provides important information concerning the morphology of the third ventricle and allows the identification of an appropriate puncture site in the floor of the third ventricle.

Comparison of magnetic resonance angiography with conventional angiography in the detection of intracranial aneurysms in patients presenting with subarachnoid haemorrhage.

Thirty-nine patients admitted with proven subarachnoid haemorrhage were imaged both with 3-D time of flight (TOF) magnetic resonance angiography (MRA) and conventional angiography. As the definitive examination, catheter angiography demonstrated 37 aneurysms; ten patients had no aneurysm, the remaining 29 patients had 37 aneurysms. We found the sensitivity of 3-D TOF MRA for the detection of aneurysms to be 81% and specificity to be 100% when the reporting radiologist inspects not only the MIP reconstructions but also the MRA source data and the axial spin-echo images.

Problems and pitfalls of 3-D TOF magnetic resonance angiography of the intracranial circulation.

3-D Time of flight (TOF) Magnetic resonance angiography (MRA) is being increasingly adopted as a technique for assessment of the intracranial circulation in neuroradiological practice. We describe our recent experience of 3-D time of flight Magnetic resonance angiography. We describe some of the problems and potential pitfalls that we have experienced employing 3-D TOF MRA in these circumstances, and the diagnostic dilemmas that have arisen.

The value of MRI in the assessment of traumatic intra-uterine adhesions (Asherman's syndrome).

Since the publication titled Amenorrhea Traumatica (atretica) by Asherman in 1948, this syndrome has been considered a well defined clinical entity. It is typically manifested by the formation of fibrous adhesions involving the uterine cavity, sometimes involving the internal cervical os. The major causes are surgical intervention of the post-partum uterus and elective termination of early pregnancy.

Measurement of fetal liver, brain and placental volumes with echo-planar magnetic resonance imaging.

Objective: To quantify accurately in utero fetal liver, brain and placental volumes using echo planar imaging, and to assess whether the technique has the potential to enhance intrauterine fetal assessment.

Design: Thirty-two singleton, complicated pregnancies were scanned using echo planar imaging, a form of magnetic resonance imaging. Pregnancies were subdivided on the basis of whether the fetus was found subsequently to have an individualised birthweight ratio above (n = 21) or below (n = 11) the 10th centile.