Effects of SV40 T antigen transduction on human corneal endothelial cell wound healing in vitro.

Objective: The aim of this study was to investigate the endothelial wound healing effects of SV40 large T and small t antigen transduction on cultured human corneal endothelial cells (HCEC).

Methods: Human corneal endothelial cells were infected with either mock solution, Ad green fluorescent protein (GFP), or Ad SV40 T/t antigen/GFP, then mechanically wounded 48 h later. The endothelial wound healing rate was quantified by an analysis of the photographs taken every 12 h after wounding.

Twenty percent alcohol toxicity on rabbit corneal epithelial cells: electron microscopic study.

Purpose: To evaluate 20% ethanol toxicity on the rabbit corneal epithelium, ethanol-treated rabbit corneas were examined with electron microscopy.

Methods: Rabbit corneas (24 eyes) were treated with 20% ethanol for 30 seconds, 1 minute, and 2 minutes by using LASEK (laser-assisted subepithelial keratectomy) instruments and then washed with sterile water. Zero time, 1, 3, 5 days after ethanol treatment, corneas were excised and examined with scanning and transmission electron microscopy (SEM and TEM).

Elevated soluble Fas in aqueous humor of patients with Behcet's uveitis: correlation with uveitis severity.

Purpose: To examine the role of soluble Fas (sFas) in patients with Behcet's uveitis.

Methods: We measured the sFas levels in both sera and aqueous humor (AH) of patients (n = 40) with uveitis and of non-uveitis controls (n = 27) using an enzyme-linked immunosorbent assay. The patients with uveitis comprised 24 with Behcet's disease, 6 pan-uveitis, 5 anterior uveitis, 2 lens-induced uveitis, 1 Vogt-Koyanagi-Harada disease, 1 sarcoidosis, and 1 retinal vasculitis.