Intranasal Immunization With Nanoparticles Containing an Protein Vaccine Candidate and a Polysorbitol Transporter Adjuvant Enhances Both Humoral and Cellular Immune Responses.

Scrub typhus, a mite-borne infectious disease, is caused by . Despite many attempts to develop a protective strategy, an effective preventive vaccine has not been developed. The identification of appropriate Ags that cover diverse antigenic strains and provide long-lasting immunity is a fundamental challenge in the development of a scrub typhus vaccine.

Protective efficacy of attenuated Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of in goats.

Background: Attenuated strain can be used as a vector to transport immunogens to the host antigen-binding sites.

Objectives: The study aimed to determine the protective efficacy of attenuated strain expressing highly conserved immunogens in goats.

Methods: Goats were vaccinated with vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination.

Protective efficacy of a vaccine using a -based delivery system expressing Omp3b, BCSP31, and SOD proteins against brucellosis in Korean black goats.

This study aimed to assess the protective efficacy of a novel vaccine formulation in goats. Twenty black goats were separated into 2 groups. Group A was injected with 3.

Protective efficacy of a canine brucellosis vaccine candidate based on live attenuated Salmonella expressing recombinant Brucella BCSP31, Omp3b and SOD proteins in Beagles.

The aim of this study was to establish a proof-of-concept of protective efficacy of Salmonella-based B. abortus vaccine candidate in Beagles. Group A Beagles (n=10) were subcutaneously (SC) inoculated with S.

Vaccination of goats with a combination vector expressing four antigens (BLS, PrpA, Omp19, and SOD) confers protection against infection.

is an intracellular pathogen with a cellular infection mechanism similar to that of , making it a suitable choice for use in an anti- immune boost system. This study explores the efficacy of a Typhimurium delivery-based combination vaccine for four heterologous antigens ( lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 10 colony-forming unit (CFU)/mL (Group B) and 5 × 10 CFU/mL (Group C) and challenged the inoculations with virulent at 6 weeks post-immunization.

Brucella abortus lysed cells using GI24 induce robust immune response and provide effective protection in Beagles.

The aim of the present study is to estimate the protective efficacy of Brucella abortus lysed cells by GI24 against brucellosis in Beagles. Group A was subcutaneously (sc) immunized with sterile phosphate-buffered saline, and group B was sc immunized with approximately 3 × 109 of the lysed cells. Brucella-LPS-specific serum IgG titers and IL-4, TNF-α and IFN-γ concentrations were investigated by enzyme linked immunosorbent assay.

Protective efficacy of a Typhimurium ghost vaccine candidate constructed with a recombinant lysozyme-PMAP36 fusion protein in a murine model.

A Typhimurium ghost vaccine was constructed with the use of a recombinant fusion protein consisting of lysozyme and porcine myeloid antimicrobial peptide 36 expressed by the overexpression system. After confirmation of its effectiveness by transmission electron microscopy the vaccine was evaluated in a murine model. Of the 60 BALB/c mice equally divided into 4 groups, group A mice were intramuscularly inoculated with 100 μL of sterile phosphate-buffered saline, and the mice in groups B, C, and D were intramuscularly inoculated with approximately 1.

Protective efficacy by various doses of a new brucellosis vaccine candidate based on Salmonella strains expressing Brucella abortus BSCP31, Omp3b and superoxide dismutase against brucellosis in murine model.

Brucella species are important etiological agents of zoonotic diseases. Attenuated Salmonella strains expressing Brucella abortus BCSP31, Omp3b and superoxide dismutase proteins were tested as vaccine candidates in this study. In order to determine the optimal dose for intraperitoneal (IP) inoculation required to obtain effective protection against brucellosis, mice were immunized with various doses of a mixture of the three vaccine strains.

Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models.

Brucella abortus cells were lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36). Next, the protection efficacy of the lysed fragment as a vaccine candidate was evaluated. Group A mice were immunized with sterile PBS, group B mice were intraperitoneally (ip) immunized with 3 × 10 colony-forming units (CFUs) of B.