Nuclear localization and functional characteristics of voltage-gated potassium channel Kv1.3.

It is widely known that ion channels are expressed in the plasma membrane. However, a few studies have suggested that several ion channels including voltage-gated K(+) (Kv) channels also exist in intracellular organelles where they are involved in the biochemical events associated with cell signaling. In the present study, Western blot analysis using fractionated protein clearly indicates that Kv1.

Hydrophobic end-gated silica nanotubes for intracellular glutathione-stimulated drug delivery in drug-resistant cancer cells.

Intracellular glutathione-triggered doxorubicin release from silica nanotubes with hydrophobic labile cap was demonstrated for the drug-resistant cancer cell treatment.

Effects of voltage-gated K+ channel blockers in gefitinib-resistant H460 non-small cell lung cancer cells.

Voltage-gated K(+) (Kv) channels are known to be associated with the proliferation of several types of cancer cells, including lung adenocarcinoma cells, and certain Kv channel blockers inhibit cancer cell proliferation. In the present study, we investigated the effects of Kv channel blockers in gefitinib-resistant H460 non-small cell lung cancer (NSCLC) cells. Treatment with dendrotoxin-κ (DTX-κ), which is a Kv1.

Confocal Raman microspectroscopic study of folate receptor-targeted delivery of 6-mercaptopurine-embedded gold nanoparticles in a single cell.

We investigate the cellular uptake behaviors and efficacy of folate-coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate-conjugated AuNPs embedded with a purine analogue cancer drug of 6-mercaptopurine (6MP) were assembled via a 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4-aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy.

Real-time monitoring of glutathione-triggered thiopurine anticancer drug release in live cells investigated by surface-enhanced Raman scattering.

We investigated in vitro and in vivo glutathione (GSH)-induced intracellular thiopurine anticancer drug release on gold nanoparticle (Au NP) surfaces by means of label-free confocal Raman spectroscopy. Direct monitoring of GSH-triggered release of 6-mercaptopurine (6MP) and 6-thioguanine (6TG) was achieved in real time. Live cell imaging technique provides a nanomolar range release of 6MP and 6TG from Au NP surfaces after the injection of external GSH.

Real-time monitoring of anticancer drug release in vitro and in vivo on titania nanoparticles triggered by external glutathione.

The anticancer drug doxorubicin (DOX) appeared to adsorb efficiently on TiO(2) nanoparticles (NPs) as evidenced by visible absorption and diffuse reflectance infrared spectroscopy data. The adsorbed drugs were found released in a controlled way by external glutathione (GSH). Fluorescence of DOX appeared to be quenched substantially by TiO(2) NPs.

Cytotoxicity of serum protein-adsorbed visible-light photocatalytic Ag/AgBr/TiO2 nanoparticles.

Photocytotoxicity of visible-light catalytic Ag/AgBr/TiO(2) nanoparticles (NPs) was examined both in vitro and in vivo. The Ag/AgBr/TiO(2) NPs were prepared by the deposition-precipitation method. Their crystalline structures, atomic compositions, and light absorption property were examined by X-ray diffraction (XRD) patterns, X-ray photoelectron (XPS) intensities, and ultraviolet-visible (UV-vis) diffuse reflectance spectroscopic tools.