[Effects of a One-Week Inpatient Health Programme for Family Caregivers and Persons in Need of Care (SVLFG Care Tandem) - A Quasi-Experimental Study].

Aim: The aim of this study was to assess the effect of a one-week inpatient health programme for family carers together with the persons in need of care (care tandem) on the well-being of family carers. Acceptance and subjective benefits were also assessed. The intervention was funded by the Social Insurance for Agriculture, Forestry and Horticulture (SVLFG).

[More free time for me? Effects of a group intervention for informal caregivers based on routine data from the SVLFG long-term care insurance].

Background: The intervention is a multiday health program of the Social Insurance for Agriculture, Forestry and Horticulture (SVLFG) for informal caregivers.

Aim Of The Work: Does the intervention increase the use of outside help to gain free time for self-care?

Material And Methods: Using a quasi-experimental within-design, we analyze annual benefit data from the SVLFG long-term care insurance for the cluster sample Bavaria from 2017 to 2020 with intervention in 2018/2019. Using fixed effects panel regressions, we determine the effect heterogeneity for care relationship, duration of care and degree of care, adjusting for period effects (intervention and comparison group: 88 and 6045 persons with 207 and 16,091 observations, respectively).

[On early red-head-style external medicine and the Confucian physician Mukai Gensho].

In 1656, at the request of the imperial commissioner Inoue Masashige Chikugo-no-kami, the neo-Confucian physician Mukai Genshō compiled medical instructions given to him by the Dejima trading-post surgeon Hans Juriaen Hancke. This was the first text on Western surgery by a trained Japanese specialist. Based on an extensive analysis of related Japanese source material, it is shown that the manuscript Komōryū geka hiyō ("Secret compendium of red-head-style external medicine"), previously considered to represent Mukai's original report, is a rather corrupted version.

Stability of a surface-bound oligonucleotide duplex inferred from molecular dynamics: a study of single nucleotide defects using DNA microarrays.

Microarray technology uses the sequence dependent hybridization (binding) affinity of surface-bound oligonucleotide strands for the quantification of complex nucleic acid mixtures. In spite of its huge potential in life science and medicine, microarray oligonucleotide hybridization remains far from being understood. Taking advantage of microarray combinatorial possibilities we show that, although surface bound, the hybridization affinities of single-base mismatched oligonucleotides can be derived from first principles using parameters from bulk.

Position dependent mismatch discrimination on DNA microarrays - experiments and model.

Background: The propensity of oligonucleotide strands to form stable duplexes with complementary sequences is fundamental to a variety of biological and biotechnological processes as various as microRNA signalling, microarray hybridization and PCR. Yet our understanding of oligonucleotide hybridization, in particular in presence of surfaces, is rather limited. Here we use oligonucleotide microarrays made in-house by optically controlled DNA synthesis to produce probe sets comprising all possible single base mismatches and base bulges for each of 20 sequence motifs under study.

Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: comparison of single base mismatches and base bulges.

Background: The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. Recent experiments indicate that the underlying principles governing DNA microarray hybridization - and in particular MM discrimination - are not completely understood. Microarrays usually address complex mixtures of DNA targets.

Optical study of DNA surface hybridization reveals DNA surface density as a key parameter for microarray hybridization kinetics.

We investigate the kinetics of DNA hybridization reactions on glass substrates, where one 22 mer strand (bound-DNA) is immobilized via phenylene-diisothiocyanate linker molecule on the substrate, the dye-labeled (Cy3) complementary strand (free-DNA) is in solution in a reaction chamber. We use total internal reflection fluorescence for surface detection of hybridization. As a new feature we perform a simultaneous real-time measurement of the change of free-DNA concentration in bulk parallel to the total internal reflection fluorescence measurement.

[The surgical license of Otaguro Gentan and its background].

After the birth of Caspar-style surgery (kasuparu-ryu geka) during the 1650s, for about three decades, certificates issued by surgeons of the Dutch East India Company proved useful to those pursuing a career as a 'Western-style physician'. Five of these 'diploma' have survived the ravages of time. Four of the five beneficiaries are well known.