Publications by authors named "Wolfgang Claassen"
Nowadays, general and specific aminopeptidases are of great interest, especially for protein hydrolysis in the food industry. As shown previously, it is confirmed that the general aminopeptidase N (PepN; EC 3.4.
View Article and Find Full Text PDFLactic acid bacteria (LAB) are auxotrophic for a number of amino acids. Thus, LAB have one of the strongest proteolytic systems to acquit their amino acid requirements. One of the intracellular exopeptidases present in LAB is the glutamyl (aspartyl) specific aminopeptidase (PepA; EC 3.
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- Recent research explored the use of a specific enzyme (CsCE) from Caldicellulosiruptor saccharolyticus to produce lactulose and epilactose from lactose in milk at two different temperatures (50°C and 8°C).
- At 50°C, a higher amount of CsCE resulted in the production of 57.7% lactulose and 15.5% epilactose after 24 hours, while at the cooler 8°C, the enzyme still effectively produced 56.7% lactulose and 13.6% epilactose after 72 hours.
- This study is significant as it's the first to demonstrate the direct enzymatic production of lactulose in milk at temperatures suitable
Knowledge of concentrations of intracellular metabolites is important for quantitative analysis of metabolic networks. As far as the very fast response of intracellular metabolites in the millisecond range is concerned, the frequently used pulse technique shows an inherent limitation. The time span between the disturbance and the first sample is constrained by the time necessary to obtain a homogeneous distribution of the pertubation within the bioreactor.
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