Fungal polysaccharides from Inonotus obliquus are agonists for Toll-like receptors and induce macrophage anti-cancer activity.

Fungal polysaccharides can exert immunomodulating activity by triggering pattern recognition receptors (PRRs) on innate immune cells such as macrophages. Here, we evaluate six polysaccharides isolated from the medicinal fungus Inonotus obliquus for their ability to activate mouse and human macrophages. We identify two water-soluble polysaccharides, AcF1 and AcF3, being able to trigger several critical antitumor functions of macrophages.

A novel SARS-CoV-2 Beta RBD DNA vaccine directly targeted to antigen-presenting cells induces strong humoral and T cell responses.

Throughout the COVID-19 pandemic, several variants of concern (VoC) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have evolved, affecting the efficacy of the approved COVID-19 vaccines. To address the need for vaccines that induce strong and persistent cross-reactive neutralizing antibodies and T cell responses, we developed a prophylactic SARS-CoV-2 vaccine candidate based on our easily and rapidly adaptable plasmid DNA vaccine platform. The vaccine candidate, referred to here as VB2129, encodes a protein homodimer consisting of the receptor binding domain (RBD) from lineage B.

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from (Scop.) Singer.

(MP) is an edible mushroom used in the treatment of diabetes, hypertension and inflammation. However, the structure and biological effects of its polysaccharides (PSs) are unclear. This study investigates the structural features of a PS complex from MP (MP-PSC), its immunomodulatory activities and effects on probiotic and pathogenic bacteria.

Structural characterization of polysaccharides from Geranium sanguineum L. and their immunomodulatory effects in response to inflammatory agents.

Ethnopharmacological Relevance: Geranium sanguineum L. is used for treatment of inflammations, anemia, malignant diseases of the blood-forming organs, diarrhea, respiratory infections, etc. Only flavonoids in root extracts have been elucidated as immunostimulating and anti-inflammatory compounds, and polysaccharides in the herb have not been examined.

Ethnopharmacology, biological activities and chemical compounds of Canarium strictum: An important resin-yielding medicinal tree in India.

The resin of Canarium strictum Roxb. is used for rheumatism and asthma; the bark is used as a mosquito repellent. The major compounds in the resin are triterpenoids, but as no studies have been performed on the bark, this study investigated this economically important resource.

Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1.

The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like receptors (TLR) and dectin-1. In the present study, Pleurotus eryngii polysaccharide fractions containing a 3-O methylated mannogalactan and (1→3)/(1→6)-β-d-glucans were isolated and extensively characterized by 2D NMR and methylation analysis. Traces of a (1→3)-α-d-glucan and a (1→2)-α-d-mannan were also observed.

The Wildland Fire Heat Budget-Using Bi-Directional Probes to Measure Sensible Heat Flux and Energy in Surface Fires.

Sensible energy is the primary mode of heat dissipation from combustion in wildland surface fires. However, despite its importance to fire dynamics, smoke transport, and in determining ecological effects, it is not routinely measured. McCaffrey and Heskestad (A robust bidirectional low-velocity probe for flame and fire application.

University Student Health and Wellbeing Study: A test-retest reliability study of a web-based survey investigating undergraduate student health and wellbeing.

Issue Addressed: Understanding the health behaviours of Australian university students and their impact on wellbeing and academic success is important; however, there are limited reliable, population level survey tools to measure student health across a range of domains. The purpose of the research was to determine the reliability of the web-based University Student Health and Wellbeing Study (USHWS) survey via a test-retest reliability study within a large Australian university student population.

Methods: A test-retest of the measurement instrument was completed by a sample of university students (n = 195) over a two-week period.

Neutral Polysaccharide from the Leaves of : Presence of 3--Methyl Galactose and Anti-Inflammatory Activity in LPS-Stimulated RAW 264.7 Cells.

(Seem.) Guillaumin is a native tree in Vietnam. The water extract of the leaves from this tree gives a highly viscous product that has been used to heal wounds and treat inflammations.

The injury workforce in Western Australia: Findings from a cross-sectional survey.

Issue Addressed: Since 1986, injury prevention and control has been classified as a National Health Priority. However, no reviews into the injury prevention workforce have been conducted in Australia since 2011 and to date; none has focused specifically on the injury prevention and safety promotion sector in Western Australia (WA). This research sought to review the scope of the injury prevention and safety promotion workforce in WA to gain a greater understanding of sector characteristics, work and needs.

Separation of Branched Poly(bisphenol A carbonate) Structures by Solvent Gradient at Near-Critical Conditions and Two-Dimensional Liquid Chromatography.

Branching is a molecular metric that strongly influences the application properties of polymers. Consequently, detailed information on the microstructure is required to gain a deeper understanding of structure-property relationships. In the present case, we employ high-performance liquid chromatography to characterize the branching in a poly(bisphenol A carbonate) (PC).

A balanced approach to falls prevention: Application in the real world.

Background: Falls place a heavy burden on the health system, impacting on an individual's quality of life, often resulting in a fear of falling, reduction in independence and at times admission to residential care. This study aimed to determine health professionals' confidence in discussing falls prevention strategies, topics discussed and the barriers and enablers to falls prevention discussions with clients aged 60 years and over.

Method: A cross-sectional self-complete online survey was undertaken with a sample of health professionals (n = 191) who had engaged in the services of the Stay On Your Feet programs delivered by the Injury Matters in Western Australia (WA).

Structural characterization of bioactive heteropolysaccharides from the medicinal fungus Inonotus obliquus (Chaga).

The aim of this paper was to perform a comprehensive characterization of polysaccharides isolated from the interior (IOI) and exterior (IOE) parts of the fungus Inonotus obliquus. Pre-extraction with DCM and MeOH, followed by water and alkali extraction and ethanol precipitation gave two water extracts and two alkali extracts. Neutral and acidic polysaccharide fractions were obtained after anion-exchange chromatography of the water extracts.

Selective chromatographic separation of polycarbonate according to hydroxyl end-groups using a porous graphitic carbon column.

Porous graphitic carbon (PGC) has shown unique separation efficiency in liquid chromatography for a wide range of substance classes. In the characterization of polymers PGC has particularly been used for analysis of polyolefins. Its retention mechanisms differ dramatically from those of silica-based stationary phases and therefore allow interesting applications.

Perceptions of Group-Based Walks and Strategies to Inform the Development of an Intervention in Retirement Villages: Perspectives of Residents and Village Managers.

The aim of the current study was to explore perceptions of group-based walking and gather suggestions to inform the development of a group-based walking intervention among older adults in retirement villages. Twenty-four physically inactive residents (16 female, 8 male; age range: 69-88) and four managers from four retirement villages were interviewed. Inductive thematic analysis revealed six broad themes: lack of motivation, values versus constraints, fears and confidence, need for structure, creating a sense of belonging, and the physical environment as a double-edged sword.

Simple Trapeziectomy for Arthrosis of the Basal Joint of the Thumb: 49 Thumbs Reviewed After Two Years.

Background: Many different surgical strategies for arthrosis of the carpometacarpal joint of the thumb are described in the literature. In 2010 we changed our routine procedure from an interposition arthroplasty using the abductor pollicis longus (APL) tendon to simple trapeziectomy without suspension or interposition. The purpose of this study was to review the clinical outcome after trapeziectomy and to compare it to those we had achieved with the APL procedure.

Backscatter near-end solution in processing of scanning lidar data.

The significant issue of the classic multiangle data-processing technique is that the height up to which this technique allows the reliable profiling of the searched atmosphere is always significantly less than the maximum operative range of the scanning lidar signals. The existing multiangle inversion methodology does not allow for the proper inversion into optical profiles of the distant range signals measured in and close to zenith. In this study, a data-processing technique is considered which allows for increasing the maximal heights when profiling the atmosphere with scanning lidar; it is achieved by using the auxiliary backscatter near-end solution and the assumption of a constant lidar ratio over high altitudes.

Determination of the smoke-plume heights and their dynamics with ground-based scanning lidar.

Lidar-data processing techniques are analyzed, which allow determining smoke-plume heights and their dynamics and can be helpful for the improvement of smoke dispersion and air quality models. The data processing algorithms considered in the paper are based on the analysis of two alternative characteristics related to the smoke dispersion process: the regularized intercept function, extracted directly from the recorded lidar signal, and the square-range corrected backscatter signal, obtained after determining and subtracting the constant offset in the recorded signal. The analysis is performed using experimental data of the scanning lidar obtained in the area of prescribed fires.

Identification of macrolide-resistant Mycoplasma genitalium using real-time PCR.

Objectives: Mycoplasma genitalium is a common cause of non-gonococcal urethritis (NGU) in Western Europe, but is not routinely tested for in all clinics. A high prevalence of macrolide-resistant M. genitalium has been reported.

Atypical temporomandibular joint pain: a case report.

Atypical temporomandibular joint (TMJ) pain can consist of an unusual intensity, location or set of pain descriptors that do not match what is traditionally observed for TMJ capsular pain, disc displacements or arthritic conditions. Presented in this case report is an atypical pain report regarding a unilateral TMJ pain as the chief complaint. An overview of typical vs atypical TMJ pain is also reviewed to highlight unusual signs and symptoms so that the clinician can identify these atypical presentations and pursue further diagnostic approaches.

The identification and quantification of a high molecular weight light stabilizer in polycarbonate by application of an online coupling of size exclusion chromatography in stopped flow mode with pyrolysis gas chromatography time of flight mass spectroscopy.

The identification and quantification of a high molecular weight light stabilizer (Uvinul 3030™) in an unknown polycarbonate sample was achieved through the application of SEC-Py-TOF-GCMS. A size exclusion column optimized to achieve resolution in the lower mass range was applied to allow the fractionation of an individual additive peak. A commercially available sampling interface was operated in stop flow mode and fractions were pyrolyzed to allow chromatographic separation of the fragments of the otherwise non-volatile stabilizer.