Publications by authors named "Wojciech Baranski"
Clinical endometritis (CE) is common in post-partum dairy cows and is associated with impaired reproductive performance. The aim of the study was to evaluate the effect of subclinical endometritis (SE) in cows clinically cured of CE on their fertility. The study was performed on 215 Holstein Friesian cows with CE diagnosed by vaginoscopy and ultrasound between 21 and 28 days after parturition.
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- The study aimed to assess the impact of a daily low dose of the GnRH agonist buserelin on hormone release and ovarian function in anovulatory dairy cows.
- Conducted on 10 Polish Holstein-Friesian cows, the treatment group received buserelin, while the control group received saline, with hormone levels measured via radioimmunoassay (RIA) and monitored for ovarian changes using ultrasound.
- Results showed that buserelin significantly increased FSH and LH concentrations, leading to more follicles and higher ovulation rates in treated cows compared to controls.
Up to 50 % of dairy cows fail to resolve uterine involution and develop chronic clinical (CE) or subclinical endometritis (SE) 21 days after calving. Clinical endometritis is associated with purulent discharge, while SE is not associated with overt clinical signs. Along with numerous knowledge gaps related to its pathogenesis, SE does not allow for a straightforward and effective therapy.
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- This study evaluated the prevalence of subclinical endometritis (SE) in cows that clinically recovered from clinical endometritis (CE) after treatments with either intrauterine cephapirin or PGFα, as well as self-healing.
- A total of 222 Polish Holstein Friesian cows were assessed, showing an average SE prevalence of 40.9% among those deemed clinically cured.
- The type of vaginal discharge influenced SE rates, with higher occurrences seen in cases associated with purulent discharge compared to mucopurulent discharge, especially in cows treated with cephapirin.
Zearalenone (ZEN) and its metabolites, alpha-zearalenol (α-ZEL) and beta-zearalenol (-ZEL), are ubiquitous in plant materials used as feed components in dairy cattle diets. The aim of this study was to confirm the occurrence of ZEN and its selected metabolites in blood samples collected from different sites in the hepatic portal system (posthepatic-external jugular vein EJV; prehepatic-abdominal subcutaneous vein ASV and median caudal vein MCV) of dairy cows diagnosed with mastitis, ovarian cysts and pyometra. The presence of mycotoxins in the blood plasma was determined with the use of combined separation methods involving immunoaffinity columns, a liquid chromatography system and a mass spectrometry system.
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- - After cows give birth, their uterus undergoes changes to prepare for future pregnancies; researchers used an algorithm to find specific mRNA patterns that indicate reproductive success.
- - They studied cows six weeks after giving birth, analyzing neutrophil percentages and taking biopsies to measure gene expression patterns, categorizing cows based on pregnancy status and neutrophil levels.
- - Findings showed that certain gene expressions varied with pregnancy timing, and specific expression ratios could predict the time from calving to conception, aiding in assessing the reproductive health of the cows.
Current knowledge about the function of the reproductive tract and appropriate use of hormonal drugs affords control of the oestrus cycle of cows. One of the hormonal protocols is OvSynch, which enables artificial insemination (AI) to be performed at the precise optimum time without control of the ovaries and uterus. Use of such protocols in reproductive management allows oestrus cycles to be synchronised and cows to be effectively inseminated without oestrous detection, which is time-consuming and difficult in farms with numerous cows.
View Article and Find Full Text PDFLittle is known about the inflammatory response of the endometrium in repeat-breeding cows with subclinical endometritis (SE). The objective of this study was to evaluate the mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS 2), prostaglandin F2 synthase (PTGFS) and prostaglandin E2 microsomal synthase 1 (mPTGES 1) in the endometrium of repeat-breeding cows with and without SE. SE was diagnosed cytologically using the cytobrush method, with the threshold being set at 5% polymorphonuclear neutrophils.
View Article and Find Full Text PDFThe aims of this study were (1) to measure the secretion of prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2), leukotriene B4 (LTB4), and leukotriene C4 (LTC4) by endometrial cells collected by a cytobrush from healthy cows and cows with subclinical and clinical endometritis in the fourth week postpartum, and (2) to evaluate the relationship between the mediators' levels of secretion and the number of polymorphonuclear neutrophils (PMNs) in the uterine smears of cows with subclinical endometritis. The study included cows without any signs of clinical endometritis (n = 63) and cows with clinical endometritis as a positive control (ENDOM, n = 12). Two different threshold ratios (>5% and >18% of PMNs) were used to categorize the cows without clinical signs as with or without cytologic endometritis (CE).
View Article and Find Full Text PDFThe activities of steroid sulphatase (StS) and estrogen sulphotransferase (EST) were determined in the epididymis of 18 boars. The animals were divided into three groups (n=6) according to age (8, 12 and 16 months). The boars were anesthetized and castrated.
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