[Diagnostic values of lactate dehydrogenase (LDH), creatine kinase (CK) and gamma-glutamyltransferase (gamma-GT) examinations in the course of intrahepatic cholestasis in pregnancy].

Objective: The activity of LDH, CK and gamma-GT in blood serum of women with intrahepatic cholestasis in pregnancy was investigated. Diagnosis of intrahepatic cholestasis was based on anamnesis, clinical examination and laboratory tests.

Methods: 41 women with intrahepatic cholestasis (the study group) and 30 healthy women (the control group) entered the study.

[The influence of chosen hormones on lipids and its correlation during normal pregnancy].

Objectives: Lipids and hormones levels in nonpregnant and pregnant with uneventful gestation (from I, II, III trimester) were estimated and its correlation was evaluated.

Methods: The study group consisted of 219 women: 49 nonpregnant and 170 pregnant women (35 in I trimester, 35 in II and 100 in III trimester of gestation). All subjects were healthy.

The biochemical functions of the renal tubules and glomeruli in the course of intrahepatic cholestasis in pregnancy.

Biochemical functions of kidney glomeruli and tubules were estimated in pregnancy complicated by cholestasis. The investigated group consisted of 72 women with pregnancy complicated by cholestasis and 30 healthy pregnant patients as a control group. Biochemical assays were performed for the deamination of amino acids, carbonic acid dissociation and creatinine metabolism.

Second trimester calcium-phosphorus-magnesium homeostasis in women with threatened preterm delivery.

Objective: The effect of threatened preterm delivery on calcium, phosphorus, magnesium homeostasis in the second trimester of pregnancy was investigated.

Methods: Serum concentrations of total and ionized calcium, inorganic phosphorus, magnesium, total protein, albumin, total estrogens and human placental lactogen were determined in women with threatened preterm delivery at 23-28 weeks of gestation (the studied group) and in women with uncomplicated pregnancy of the same duration (the control group). Additionally activities of total alkaline phosphatase and heat-stable alkaline phosphatase fraction were measured.

Calcium-phosphorus-magnesium homeostasis in women with threatened preterm delivery.

Objective: The effect of threatened preterm delivery on calcium, phosphorus, magnesium homeostasis in the third trimester of pregnancy was investigated.

Methods: Serum concentrations of total and ionized calcium, inorganic phosphorus, magnesium, total protein, albumin, total estrogens and human placental lactogen were determined in women with threatened preterm delivery at 29-36 weeks of gestation (the studied group) and in women with uncomplicated pregnancy of the same duration (the control group). Additionally, activities of total alkaline phosphatase and heat-stable alkaline phosphatase fraction were measured.

[Calcium, phosphorus and magnesium in pregnant women with primary hypertension].

Sixty five women were in the third trimester of pregnancy (29-40 weeks of gestation) submitted to the study including 35 with primary hypertension (the studied group) and 30 healthy (control group). The following parameters were measured in blood serum and urine from 24 hrs, collection: total Ca and Ca++, inorganic phosphorus (Pi) and magnesium. Generally accepted micromethods were used; Ca++ was measured using AVL type 9140 analyser.

[Calcium-phosphorus-magnesium homeostasis in women with threatened abortion].

One hundred twenty eight women were submitted to research including: 38 healthy not pregnant women, 40 healthy women in the first trimester of uncomplicated pregnancy (6-15 weeks) and 50 pregnant women with symptoms of threatened abortion (6-15 weeks). The following parameters were measured in serum: total Ca, Ca++, ionised inorganic phosphorus (Pi), magnesium (Mg), total protein and albumin and also total alkaline phosphatase activity (APt). Micromethods generally accepted in clinical laboratories were used.

[Evaluation of the intrauterine fetus in hypertension--biochemical assessment of amniotic fluid. IV. Ions].

Sixty two samples of amniotic fluid, collected by ultrasound guided amniocentesis, were submitted to biochemical investigation including 31 samples from women with pregnancy complicated by hypertension (studied group) and 31 samples deriving from healthy pregnant women (control group with). Both investigated and control groups consisted of pregnant women with the same gestational age of 37 +/- 2 weeks (34-40 weeks). The following ions were measured: NH4+, K+, Na+, Cl-, Mg++, total Ca, Ca++ and inorganic phosphorus (Pi).

[Evaluation of fetal condition in pregnancy complicated by hypertension--biochemical assessment of amniotic fluid. III. Acid-base balance].

Sixty two samples of amniotic fluid, collected by ultrasound guided amniocentesis, were submitted to biochemical investigation including 31 samples from women with pregnancy complicated by hypertension (studied group) and 31 samples deriving from healthy pregnant women (control group with). The following parameters of acid-base balance were measured in amniotic fluids of both groups: pH, pCO2, base deficiency, standard HCO3 and total CO2. Corning device type 168 was used.

[Evaluation of fetal condition in pregnancy complicated by hypertension--biochemical assessment of amniotic fluid. II. Enzymes].

Sixty two samples of amniotic fluid were submitted to biochemical investigation including 31 samples from women with pregnancy complicated by hypertension (studied group with blood pressure -65 +/- 15/95 +/- 5 mm Hg) and 31 samples deriving from healthy pregnant women (control group with mean blood pressure 118 +/- 10/74 +/- +/- 9 mm Hg). The following parameters of amniotic fluid were measured: 1) aminotransferases: alanine AlAT and aspartate AspAT, 2) alkaline phosphatase (APt) and its thermostable isoenzyme (APh), 3) ceruloplasmin (Crlp), 4) alpha-amylase (alpha-Amy). The study showed pregnancy complicated by hypertension is related to fetal salivary gland's immaturity presenting decreased activity of alpha amylase in amniotic fluid.

[Evaluation of fetal condition in pregnancy complicated by hypertension--biochemical assessment of amniotic fluid. I. Protein and lipid components and kidney maturation].

Sixty two samples of amniotic fluid were submitted to biochemical investigation including 31 samples from women with pregnancy complicated by hypertension (studied group with mean blood pressure 165 +/- 15/95 +/- 5 mmHg) and 31 samples deriving from healthy pregnant women (control group with mean blood pressure 118 +/- 10/74 +/- +/- 9 mmHg). In the studied group pregnancy induced hypertension, primary hypertension and nephrogenic hypertension scored 58%, 32% and 10% of cases respectively. Both investigated and control groups consisted of pregnant women with the same gestational age of 37 +/- 2 weeks (34-40 weeks).

[Homeostasis of calcium-phosphorus-magnesium in women with pregnancy induced hypertension].

Study group consisted of 63 women in the III trimester of pregnancy (gestational age 29-40 weeks). 32 subjects with PIH (investigated group) were compared to control group consisted of 30 healthy patients with uneventful course of gestation. Women with PIH had no proteinuria nor oedema, their mean blood pressure remained at the level of 161 +/- 16.

[Lipid metabolism in women with threatened abortion].

The study group consisted of 130 subjects: 50 healthy nonpregnant, 35 healthy women in the I trimester of gestation (gestational age 6-15 weeks) and 45 patients with symptoms of threatened abortion (the same gestational age). In the latter group pain and bleeding ceased after hormonal treatment and spasmolytic drugs. Serum blood concentration of following compounds were determined: total lipids, LDL fraction, total cholesterol, phospholipids, triglycerides, HDL and LDL cholesterol fractions.

[Bile acids in blood serum of women during normal pregnancy, early puerperium and in the neonate].

Total bile acids in serum blood samples from 325 subjects were estimated. Study group consisted of 50 nonpregnant women, 40-in the I trimester, 50-in the II and 100-in the III trimester of gestation, 55 patients in the third day of puerperium and 30 newborn infants (cord serum). All women were healthy, their age varied between 20 and 36 years.

[The effect of total estrogens and lactogen hormone on lipid metabolism in women during normal pregnancy].

Lipids parameters and total oestrogen and HPL levels were estimated in blood serum. Investigated group consisted of 45 women with uneventful course of pregnancy. All pregnant women were in the gestational age between 29 and 40 weeks.

[Fetal metabolism in post-term pregnancy based on biochemical examinations of amniotic fluid. V. Glucose, bilirubin, H+ ions, hormones and tests of lung maturation].

Concentrations of glucose, bilirubin, H+ ions, estriol, HPL were determined in the samples of amniotic fluid from post term and near term pregnancies. Investigated group consisted of samples taken between 42-43 week of gestation, control group between 38-40 week. In both groups biochemical tests of pulmonary maturation--Lectin/Sphingomyelin ratio, foam test, 650 nm absorbance were carried out.

[Fetal metabolism in post term pregnancy based on biochemical investigations of amniotic fluid. IV. Ion composition].

Concentrations of calcium, sodium, magnesium, calcium (total and ionised) and inorganic phosphorus were determined in amniotic fluid. Samples were taken from patients between 42-43 week of gestation (investigated group) and between 38 and 40 week (control group). Micro-methods, generally acknowledged in laboratories of clinical biochemistry were used.

[Concentration of lipids and lipoproteins in serum of women with pregnancy induced hypertension].

The tests have been carried out on 64 women in 3rd trimester of pregnancy. 30 of them were the women with a normal course of pregnancy, and 34 were the ones with hypertension induced pregnancy (PIH). Their blood serum has been tested for the concentration of TG, phospholipids, total cholesterol, HDL- and LDL-cholesterol, alpha, beta and pre-beta lipoprotein fractions.

[Concentration of lipids and lipoproteins in serum of pregnant women with primary hypertension].

The tests have been conducted on 44 women in 3rd trimester of pregnancy. 30 of them were the women with a normal course of pregnancy, and 14 were the ones with the primary hypertension. The blood serum has been tested for the concentration of triglycerides, phospholipids, total cholesterol, HDL and LDL- cholesterol, alpha, beta and pre-beta lipoprotein fractions.

[Activity of alpha-amylase in serum and urine of pregnant women with diabetes mellitus type I].

The investigations covered 33 women in the III trimester of pregnancy with diagnosed, insulin-dependent diabetes mellitus, and 108 healthy women in the control group. The alpha-amylase activity was measured in the blood-serum and urine samples using the Caravay technique. An increased activity was demonstrated in the blood-serum samples in the examined group.

[Alpha-amylase in serum and urine of women with hypertension during the III trimester of pregnancy].

The urinary and blood serum activity of alpha-amylase was tested in 34 women with arterial hypertension in the III trimester of pregnancy (examined group). The results were compared with a control group of healthy women (35 urine samples and 108 blood-serum samples). In both groups other biochemical examinations were carried additionally (the serum creatinine, urea, uric acid, electrolytes and the acid-base balance levels were established).

[Kidney function in pregnant women with hypertension in the course of chronic kidney disease].

In 53 pregnant patients in the III trimester of pregnancy kidney function investigations were carried. The group consisted of 23 patients with chronic kidney diseases with superimposed arterial hypertension (examined group) and of 30 healthy pregnant women (control group). In the examined group an increase of blood-serum urea, uric acid and creatinine concentrations were demonstrated.

[Kidney function in pregnant women with primary arterial hypertension].

Investigations covered 64 women in the III trimester of pregnancy. In this group 34 were with diagnosed primary arterial hypertension (examined group), and 30 were apparently healthy (control group). In both groups the blood serum concentrations of creatinine, urea, uric acid and electrolytes were determined.

Carbohydrate metabolism in the course of intrahepatic cholestasis in pregnancy.

Glucose metabolism was evaluated in pregnant women with clinically and biochemically demonstrated intrahepatic cholestasis. Laboratory investigations included measurements of serum glucose concentrations on fasting and 2 hours after breakfast, the glucose tolerance test (100 gm oral glucose load), and 24-hour glycemia profile. All patients were admitted to the II Department of Obstetrics and Gynecology, Institute of Obstetrics and Gynecology of the Medical School in Warsaw, Poland.

[Clinical condition of the newborns and the type of labor and pregnancy complications in cases of meconium in the amniotic fluid].

The studied material comprised 36 newborns and their mothers, among them 12 newborns had meconium in the amniotic fluid (study group) and 24 newborns with clear amniotic fluid served as controls. The fluids were studied biochemically. In both compared groups the analysed factors were: 1) week of labour, 2) body weight and length, 3) clinical condition of the newborn by the Apgar score, 4) type of labour, 5) type of pregnancy complications in mothers.