[Inhibition of leukocyte migration after concanavalin A and phytohemagglutinin in patients with ulcers].

An attempt of the assessment of T-cells function in patients with gastric or duodenal ulcer has been undertaken. The studies involved 60 patients with gastric or duodenal ulcers and 47 individuals of the control group. Lymphocyte reactivity to different concentrations of concanavalin A and phytohemagglutinin has been assessed with leukocyte migration inhibition test.

[The effect of in vitro theophylline on T lymphocytes from patients with ulcers].

An increase in the interest in the cellular immunity in patients with peptic and duodenal ulcers dates back to the detection of histamine H2 receptors on T-cells. In vitro effect of theophylline on T-cells in patients with peptic and duodenal ulcers was investigated. The experiment included 107 patients: 60 with peptic and duodenal ulcers and 47 controls.

Theophylline-resistant and theophylline-sensitive "active" and "total" E rosette-forming lymphocytes in patients with systemic scleroderma.

Using the E rosette test and its modification with theophylline, we have studied T regulatory lymphocytes in various forms of systemic scleroderma. Mean percentages of active rosette-forming cells (ARFC) as well as the fraction resistant to theophylline incubation (ARFC-res) were significantly decreased, irrespective of the variety of the disease, compared to the age-matched controls. Late ("cold") rosette-forming fractions were unimpaired.

A contribution to studies on the mechanism of lymphocyte-induced angiogenesis: human peripheral blood mononuclear leucocytes with highest angiogenic activity.

Using the lymphocyte-induced angiogenesis test a comparison of angiogenic activity of some mononuclear cell fractions was performed. It is shown that the highest angiogenic activity was expressed by cells bearing both the receptor for the Fe portion of IgG and a CD4 surface antigen.

Modulatory effect of sera from scleroderma patients on lymphocyte-induced angiogenesis. II. Effector cells for the enhancing effect of acroscleroderma patients' sera.

In our previous study, using the modified lymphocyte-induced angiogenesis assay, we found that incubation of normal human peripheral blood mononuclear cells (PBMCs) with sera from patients with acroscleroderma, but not with diffuse scleroderma, markedly enhanced their angiogenic capability. The results of the present work suggest that this enhancement is mediated by lymphocytes bearing receptors for the Fc portion of IgG and belonging mainly to the CD4+ T-cell subset.

Theophylline-resistant and theophylline-sensitive "early" and "late" E rosette-forming cells in young and aged humans.

The percentage of cells with high-affinity sheep red blood cell (SRBC) receptors, "active" or "early" rosette-forming cells, (ARFC) in theophylline-resistant peripheral blood T lymphocytes achieve higher values in aged subjects and, in particular, in the group with clinically manifested atherosclerosis. A diminished OKT-4/OKT-8 ratio in theophylline-resistant ARFC was noticed in this group. On the contrary, the proportion of the theophylline-resistant cells with low-affinity receptors, cold or late rosette-forming cells (CRFC) attained lower values in aged subjects than in young subjects.

Altered immune reactivity of mice born from mothers treated with ampicillin during gestation.

The cellular and humoral immune responses of 30-day-old mice born from mothers treated with ampicillin during pregnancy were studied. A lowering of cellular immunity, accompanied by a decrease in T lymphocyte percentage in lymph nodes, and an increase of humoral immune response were observed.

The effect of vitamin E treatment on the incidence of OKT+4 lymphocytes in the peripheral blood of children with chronic respiratory tract infections.

Children with respiratory tract infections treated for 6 weeks with vitamin E improved their clinical status and normalized the proportion of OKT+4 T lymphocytes and the ratio of OKT+4 to OKT+8 cells in their peripheral blood.

Studies on the influence of adrenaline, acetylcholine and cysteine on suppressive activity of lymphocytes engaged in GvH reaction in rats.

Thirty-minute preincubation of lymphocytes deriving from popliteal lymph nodes of hybrid W X A F1 rats experiencing GvH reaction in 10(-6)M solution of acetylcholine resulted in an increased [3H]-thymidine incorporation both in cultures stimulated and nonstimulated with PHA. When the cells were preincubated for 30 min in 10(-5)M solution of adrenaline the level of [3H] TdR incorporation into non-stimulated cells was increased. The cells pretreated in such a way and added to cultures containing normal peripheral lymph node lymphocytes responding to PHA exhibited diminished or even abrogated suppressive effect which they normally exert if they are not treated with these two drugs.