Publications by authors named "Wladimir Padilha da Silva"

The study aimed to evaluate the effects of supplementation with Lacticaseibacillus casei CSL3 in Swiss mice immunosuppressed with cyclophosphamide on immunological, biochemical, oxidative stress, and histological parameters. The animals were distributed into four groups (control, CSL3, cyclophosphamide, and CSL3 + cyclophosphamide), where two groups were treated with L. casei CSL3 (10 log CFU mL) for 30 days, and two groups received chemotherapy (days 27 and 30-total dose of 250 mg kg).

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This study aimed to evaluate the ability of biofilm formation by L. monocytogenes from the meat processing industry environment, as well as the use of different combinations of detergents, sanitizers, and UV-A radiation in the control of this microorganism in the planktonic and sessile forms. Four L.

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Aims: To develop and characterize a functional lactose-free ice cream with added ginger and honey, evaluate the survival of Lacticaseibacillus casei CSL3 under frozen storage and the simulated gastrointestinal tract (GIT), as well as antioxidant activity and product acceptability.

Methods And Results: The survival of Lacticaseibacillus casei CSL3 was evaluated for 180 days, under frozen storage, and GIT at 60 days. At 15 days of storage, proximal composition, antioxidant activity, color, pH, acidity, fusion, density, overrun, and sensory analysis were performed.

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Unlabelled: This study aimed to investigate the occurrence and the genetic diversity of subsp. in sausages from Southern Brazil, evaluate virulence genes and determine the phenotypic and genotypic basis of antimicrobial and sanitizer resistance. was detected in sausage samples with an overall prevalence of 5.

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This study aimed to develop a green method to obtain an anthocyanin-rich edible extract of Clitoria ternatea flowers grown in southern Brazil. The extract was characterized by UHPLC-MS and analyzed for toxicological potential in an in vivo model, total phenolic content, and biological activities. By using a 2 multivariate design to study the effects of temperature, acidified solvent, and time on the total anthocyanin content (487.

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Pediococcus pentosaceus is a lactic acid bacterium that has probiotic potential proven by studies. However, its viability can be affected by adverse conditions such as storage, heat stress, and even gastrointestinal passage. Thus, the aim of the present study was to microencapsulate and characterize microcapsules obtained by spray drying and produced only with whey powder (W) or whey powder combined with pectin (WP) or xanthan (WX) in the protection of P.

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The aim of the work was to evaluate antagonistic activity of Lactobacillus spp. and Bifidobacterium spp. in vitro against cariogenic Streptococcus mutans UA 159 and viability in chewing gum, during storage.

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Article Synopsis
  • This study used a label-free proteomics method to identify proteins in Campylobacter jejuni that respond to different pH levels, focusing on the reference strain NCTC 11168.
  • Researchers grew the bacteria at normal pH levels (5.8, 7.0, and 8.0) and exposed it to a lower pH (4.0) shock, finding specific proteins that increased in abundance under acidic conditions even without activation from acid shock.
  • The results indicate that C. jejuni adjusts its respiration and metabolism in response to pH changes, enhancing growth and energy conservation, which contributes to its competitiveness and overall fitness.
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Abstract: The traffic in international animal products can become a public health hazard when legal import sanitary procedures are not followed. In Brazil, due to its extensive border area, the importation of animal products is a common practice in many areas, especially in Rio Grande do Sul, a state that borders Argentina and Uruguay. The objective of this study was to evaluate the presence of veterinary drug residues (antibiotics and antiparasitics) in animal products consumed in Rio Grande do Sul.

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Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression. This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp. cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model.

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 spp causes foodborne diseases related to the consumption of contaminated foods, especially poultry products. This study aimed to investigate the occurrence of spp. serovars in raw eggs from supermarkets and street food markets in southern Brazil, to analyze virulence genes, resistance profiling to antimicrobials and sanitizers, and to determine the susceptibility of the isolates to extract.

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Survival of Lactococcus lactis subsp. lactis R7, microencapsulated with whey and inulin, was analyzed when added to blueberry juice, milk, and cream. For 28 days, cell viability was evaluated for storage (4 °C), simulated gastrointestinal tract (GIT), and thermal resistance.

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Article Synopsis
  • * Results showed that isolates were categorized into two main lineages, with lineage I displaying higher invasion capabilities in human cells and expressing a specific virulence factor called InlA.
  • * isolates from lineage II exhibited lower invasion rates due to a lack of InlA expression or mutations that caused malfunction, indicating differences in virulence potential linked to the isolates' genetic backgrounds.
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Campylobacter jejuni is a highly frequent cause of gastrointestinal foodborne disease in humans throughout the world. Disease outcomes vary from mild to severe diarrhea, and in rare cases the Guillain-Barré syndrome or reactive arthritis can develop as a post-infection complication. Transmission to humans usually occurs via the consumption of a range of foods, especially those associated with the consumption of raw or undercooked poultry meat, unpasteurized milk, and water-based environmental sources.

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Staphylococcus aureus is one of the most common pathogens associated with food poisoning, which is caused by the ingestion of food contaminated with staphylococcal enterotoxins (SE). Our study aims at evaluating the occurrence and expression of five SE genes (sea, seb, sec, sed, and see) in S. aureus previously isolated from broiler carcasses.

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This study investigated the ability of isolates from milk to form biofilm, through detection of adhesion genes, investigating exopolysaccharide (EPS) production and biofilm formation on polystyrene (PS) and stainless steel (SS) surfaces, and by quantifying the expression of and genes under different temperatures and culture media. Among the 31 isolates, the adhesion genes and were found in 81% and 61% of the isolates, respectively. The screening tests for phenotype revealed that 58% of the isolates were EPS producers, and 45% showed the ability to produce biofilm on PS.

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Article Synopsis
  • - The study assessed the biofilm formation ability of poultry isolates under various conditions, including different surfaces (stainless steel, polystyrene), temperatures (7, 25, and 42 °C), and oxygen levels (microaerophilic and aerobic).
  • - All isolates successfully formed biofilm on both surfaces across the tested conditions, particularly thriving in dual-species scenarios where biofilm formation increased significantly at lower temperatures (7 °C).
  • - Ten genes related to biofilm formation were identified in the isolates, with some detected across all samples, raising concerns about the ability of these poultry isolates to form resilient biofilms under diverse environments.
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  • The study examined sushi from Pelotas, Brazil, to assess the presence of Listeria monocytogenes, a pathogen linked to ready-to-eat fish products.
  • They found L. monocytogenes in 21.4% of sushi samples from two establishments, and all isolates belonged to the virulent serotype 4b.
  • The research highlighted biofilm-forming abilities of the isolates on stainless steel and raised concerns about the potential risk of listeriosis from sushi consumption, emphasizing the need for better sanitation practices in sushi restaurants.
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Abstract: We aimed to perform serotyping and the antimicrobial resistance profile of Salmonella spp. and Listeria monocytogenes strains isolated from raw meats imported illegally into Brazil along the borders of Argentina and Uruguay. Distinct isolates of Salmonella spp.

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Article Synopsis
  • The study aimed to assess the prevalence of thermophilic Campylobacter in broiler farms and slaughterhouses in southern Brazil, revealing that 53.6% of samples tested positive for C. jejuni.
  • Resistance to certain antibiotics was significant, with 74% of isolates resistant to nalidixic acid and 67.3% to enrofloxacin, but no resistance was found to macrolides recommended for treating human infections.
  • Genetic analysis identified eight distinct patterns of C. jejuni, all carrying major virulence genes, indicating that the pathogen poses a risk to consumers by contaminating chicken products and potentially causing illness.
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The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil.

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Campylobacter jejuni is the most common bacterial cause of foodborne diarrheal disease worldwide and is among the antimicrobial resistant "priority pathogens" that pose greatest threat to public health. The genomes of two C. jejuni isolated from poultry meat sold on the retail market in Southern Brazil phenotypically characterized as multidrug-resistant (CJ100) and susceptible (CJ104) were sequenced and analyzed by bioinformatic tools.

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The aim of this study was to isolate from raw milk samples, to characterize its antimicrobial metabolites, and to evaluate its viability in a probiotic Minas Frescal cheese. For this, antagonist activity against , safety aspects and biochemical, genotypic, and probiotic characteristics of the isolates were evaluated. Minas Frescal cheese was manufactured with the isolate that showed the best characteristics in vitro, and its viability in the product was evaluated.

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The development of standardized and safe food products with the typical characteristics of each region is highly desirable and can be obtained by using native starter cultures that influence the flavor, texture, and color of fermented foods. Therefore, scientists have been employing various techniques for screening and characterizing native bacteria (lactic acid bacteria and Gram-positive catalase-positive cocci) for application in fermented meat sausage. The present review outlines in vitro assays that evaluate the potential application and safety aspects of native isolates and introduces emerging omics technologies applied to the microbiology of fermented meat sausage.

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The objective of this study was to compare the gene expression levels of the agr locus and prfA gene during adhesion and biofilm formation by four L. monocytogenes isolates (2 biofilm-forming and 2 non-forming) on stainless steel and polystyrene surfaces at different temperatures (10 °C, 20 °C and 37 °C), and times (8 h, 12 h, 24 h and 48 h). The agrA and prfA genes were expressed at higher levels than the agrBCD genes.

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