Publications by authors named "Witeska M"

Article Synopsis
  • Klebsiella pneumoniae is increasingly resistant to antibiotics, posing treatment challenges for infections in both humans and animals.
  • Farm animals, particularly poultry, cattle, and pigs, are significant sources of this antibacterial-resistant bacteria, with varying resistance patterns based on animal type and geographic location.
  • Notably, high resistance rates were observed in pigs and in regions like Africa and Asia, especially against last-resort antibiotics like carbapenems and polymyxins, raising concerns for public health.
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Pollution of the aquatic environment with heavy metals is a serious environmental problem, since they accumulate in aquatic organisms and can affect their development and worsen their condition. According to the scheme of Fig. 1 zinc (Zn), copper (Cu) or lead (Pb) were studied when exposed to concentrations of: Zn (0.

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Hematological analysis is commonly used to assess the physiological state of fish. It includes red blood cell parameters, white blood cell parameters, and the number of thrombocytes per blood volume unit. Hematological analysis is one of the basic tools (often accompanied by biochemical and histopathological analyses) to assess the influence of organic and inorganic substances on fish.

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Klebsiella pneumoniae (KP) is a nosocomial pathogen causing difficult-to-treat infections. The presence of virulence genes and antibiotic resistance of 109 KP isolates from hospitalized patients were investigated. Among them, 68.

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Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for hard-to-treat infections. The presence of 19 virulence genes in 120 MRSA isolates obtained from hospitalized patients and genetic relationships of these isolates were investigated. The eno (100%) and ebps (93.

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The effects of two potential antibacterial agents of plant origin: -cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against spp. developed earlier in study.

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Article Synopsis
  • Hexavalent chromium (Cr(VI)) is known to have harmful effects including cancer and toxicity to organs such as the kidneys, liver, and nervous system.
  • The study focused on the impact of Cr(VI) on developing chicken embryos, finding that higher doses significantly reduced the hatchability of chicks, while lower doses did not show major changes.
  • Even at potentially harmful levels, lower doses of Cr(VI) did not appear to cause notable hematological or liver changes in newly hatched chicks.
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Hematological, biochemical and hematopoietic effects of therapeutic doses of two antibiotics, oxytetracycline (OTC) and gentamicin (GEN), in clinically healthy common carp juveniles were studied. The fish were divided into four groups: controls 1 and 2 (untreated or injected with 0.6% NaCl solution), and two groups treated with antibiotics (orally with 75 mg/kg OTC four times every two days or injected with a single dose (4 mg/kg) of GEN dissolved in 0.

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Antibacterial agents are commonly present in aquatic environment at low concentrations. Terrestrial animal farms, human medicine and aquaculture are main sources of water contamination with antibacterials. Antibiotics were proved to be directly toxic to fish causing oxidative stress, general stress response, histopathological lesions, hematological, metabolic, and reproductive disorders, as well as immunosuppressive and genotoxic effects.

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Methicillin-resistant Staphylococcus aureus (MRSA) are among the most important biofilm-forming pathogens responsible for hard-to-treat infections. Looking for alternatives to antibiotics that prevent biofilm formation, we investigated the effects of manuka honey on the transcriptional profile of genes essential for staphylococcal biofilm formation using qRT-PCR. mRNA from two hospital MRSA strains (strong and weak biofilm producer) were isolated after 4, 8, 12 and 24 h from cells grown in biofilm.

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Pesticides are widely used in the world agriculture, and they may adversely affect non-target organisms, including fish. The present 2000-2019 literature review summarizes hematological and blood biochemical effects of various herbicides, insecticides, and fungicides in fish. The observed changes usually indicate anemia and inflammation, as well as hyperglycemia, hypoproteinemia, increase in cortisol concentration and activities of hepatic aminotransferases that are typical for intoxication and stress.

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The effects of -cinnamaldehyde (TC) on transcriptional profiles of biofilm-associated genes and the metabolic activity of two methicillin-resistant (MRSA) strains showing a different degree of adherence to polystyrene, were evaluated. Metabolic activity of in biofilm was significantly decreased in the presence of TC at 1/2 minimum biofilm inhibition concentration (MBIC). Expression levels of the genes encoding laminin binding protein (), elastin binding protein () and fibrinogen binding protein () in the presence of TC at 1/2 MBIC were lower than in untreated biofilm in both the weakly and strongly adhering strain.

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Antimicrobial activities of phytochemicals--cinnamaldehyde (TC), ferulic acid (FA), -coumaric acid (-CA), caffeic acid (CA), chlorogenic acid (CHA), essential oil (TO), essential oil (ECO), and oil (TTO) against species-were assessed. Growth of all subsp. and almost all strains was inhibited by TC at concentration 0.

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Phenylurea herbicides such as linuron are commonly applied in agriculture. Common carp juveniles were subjected to 31.5 µg/L of linuron for 14 days, and then to 30 days of purification.

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The effects of Roundup (glyphosate-based herbicide) on peripheral blood parameters and hematopoietic tissue cellular composition and activity in common carp juveniles were evaluated. The fish were exposed for 7 days at 0.0, 0.

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Objective: To evaluate the effects of handling alone versus handling under anaesthesia with 2-phenoxyethanol or etomidate on haematological parameters in carp.

Study Design: Prospective, randomized, laboratory experiment.

Animals: Seventy-two juvenile carp (Cyprinus carpio) weighing 35.

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The effects of Cd and Cu on embryos and larvae of the ide Leuciscus idus were evaluated. The ide is an European cyprinid fish, natural populations of which tend to decrease. The ide is also used as a bioindicator organism to evaluate water quality.

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The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100% 96hLC₅₀ or 10% 96hLC₅₀, respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure).

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Early developmental stages of fish are particularly sensitive to water pollution. Heavy metals may affect various developmental processes during the embryonic period, which results in a reduction of offspring quantity and quality. Waterborne metals may accumulate in the gonads of spawners and adversely affect gamete production and viability, or exert direct toxic influence upon developing embryos.

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Hematological parameters (Ht, Hb, RBC, WBC, PLT), erythrocyte size, and osmotic fragility, differential leukocyte count, ROS production in common carp blood collected on three anticoagulants: heparin (10 IU/mL, Na2EDTA (0.1, 0.5, and 1 mg/mL), and sodium citrate (0.

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The in vitro effects of cadmium, copper, lead and zinc, and various cadmium compounds (chloride, sulphate and nitrate) on common carp (Cyprinus carpio) lymphocyte viability and phagocyte activity, were evaluated. The percentage of dead lymphocytes was determined after Trypan blue staining, and phagocyte activity was measured by using the nitroblue tetrazolium (NBT) reduction test. Lead was the most toxic to lymphocytes--the maximum mortality exceeded 30%, and was significantly higher at 1 microM of lead, compared to the control.

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Blood zinc level, hematological parameters and blood cell morphology were evaluated in common carp immediately after 3 h exposure to 20 mg dm(-3) of zinc (Zn0), and in 24, 48 and 96 hours after the end of it (Zn24, Zn48, Zn96). Blood zinc level in the non-exposed fish was 8 mg dm(-3), reached a maximum of 20 mg dm(-3) in Zn48, while it dropped to 9 mg dm(-3) in Zn96. Zinc caused a stress reaction in fish indicated by an increase in hematocrit value in Zn0, and elevated plasma glucose level and trombocytosis which persisted until the end of the experiment.

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