TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis.

Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and activate innate and adaptive immune responses. Single nucleotide polymorphisms (SNPs) within the TLR genes may influence host-pathogen interactions and can have an impact on the progression of infectious diseases. The present study aimed to investigate the genotype distribution of TLR2 (2029C/T, rs121917864; 2258G/A, rs5743708), TLR4 (896A/G, rs4986790), and TLR9 (- 1237T/C, rs5743836; - 1486T/C, rs187084; 1174G/A, rs352139; and 2848C/T, rs352140) polymorphisms in 149 children and adolescents with infectious mononucleosis (IM) and 140 healthy individuals.

Distribution of the CMV glycoprotein gH/gL/gO and gH/gL/pUL128/pUL130/pUL131A complex variants and associated clinical manifestations in infants infected congenitally or postnatally.

Human cytomegalovirus (CMV) is a major cause of morbidity in fetuses following intrauterine infection. The glycoprotein (g) envelope trimeric gH/gL/gO and pentameric gH/gL/pUL128/pUL130/pUL131A complexes are required for CMV entry into fibroblasts and endothelial/epithelial cells, respectively, and both are targets for neutralizing antibodies. The role of sequence variability among viral strains in the outcome of congenital CMV infection is controversial.

Association of TLR3 L412F Polymorphism with Cytomegalovirus Infection in Children.

Intracellular Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA (dsRNA) and activates antiviral immune responses through the production of type I interferons (IFNs) and inflammatory cytokines. This receptor binds to dsRNA molecules produced during human cytomegalovirus (HCMV) replication. TLR7 senses viral single-stranded RNA (ssRNA) in endosomes, and it can interact with endogenous RNAs.

TLR9 -1486T/C and 2848C/T SNPs Are Associated with Human Cytomegalovirus Infection in Infants.

Toll-like receptor 9 (TLR9) recognizes non-methylated viral CpG-containing DNA and serves as a pattern recognition receptor that signals the presence of human cytomegalovirus (HCMV). Here, we present the genotype distribution of single-nucleotide polymorphisms (SNPs) of the TLR9 gene in infants and the relationship between TLR9 polymorphisms and HCMV infection. Four polymorphisms (-1237T/C, rs5743836; -1486T/C, rs187084; 1174G/A, rs352139; and 2848C/T, rs352140) in the TLR9 gene were genotyped in 72 infants with symptomatic HCMV infection and 70 healthy individuals.

Mannose-binding lectin in chronic hepatitis C in children.

Objective: To investigate effect of mannose-binding lectin (MBL) genetic polymorphisms and phenotype in chronic hepatitis C and its impact on response to antiviral therapy in children.

Methods: Fifty four children with chronic hepatitis C, aged 2.5-18 years were enrolled.

Human cytomegalovirus UL55, UL144, and US28 genotype distribution in infants infected congenitally or postnatally.

Cytomegalovirus (CMV) is the most common cause of congenital infection. This pathogen exhibits extensive genetic variability in the genes that encode structural envelope glycoproteins, regulatory proteins, and proteins that contribute to immune evasion. However, the role of specific viral strains in the outcome of congenital CMV infection is unclear.

Cytomegalovirus alpha-chemokine genotypes are associated with clinical manifestations in children with congenital or postnatal infections.

Human cytomegalovirus (HCMV) is the leading cause of congenital infections. The aim of our study was to determine the prevalence of genotypes based on the highly polymorphic UL146 and UL147 HCMV genes and the relationship between the genotype and symptoms or viral load. We analyzed samples from 121 infants with symptomatic HCMV infection, including 32 congenitally infected newborns.

Relationship between toll-like receptor 2 Arg677Trp and Arg753Gln and toll-like receptor 4 Asp299Gly polymorphisms and cytomegalovirus infection.

Objectives: The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults.

Methods: The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments.

Cytomegalovirus glycoprotein H genotype distribution and the relationship with hearing loss in children.

Cytomegalovirus (CMV) is a leading cause of congenital infection and a leading infectious cause of hearing loss in children. The ORF UL75 gene encodes envelope glycoprotein H (gH), which is essential for CMV entry into host cells and the target of the immune response in humans. However, the distribution of gH variants and the relationship between the viral genotype, viral load, and sequelae in children infected with CMV is debated.

Efficacy of pegylated interferon α-2b and ribavirin in chronic hepatitis C virus (genotypes 1 and 4) infection.

Background And Objective: The course of chronic hepatitis C in children is often mild or asymptomatic, but may lead to liver cirrhosis and neoplasm. The aim of our study was retrospective evaluation of treatment efficacy using pegylated interferon (IFN)-α2b with ribavirin in children and adolescents with chronic hepatitis C, both treatment naïve and re-treated.

Methods: The study comprised 79 patients with chronic hepatitis C ages 8 to 18 years (43 patients re-treated; 54 infected with genotype 1 hepatitis C virus and 25 with genotype 4), treated with pegylated IFN-α2b (1.

Distribution of cytomegalovirus gN variants and associated clinical sequelae in infants.

Background: Human cytomegalovirus (HCMV) is the most widespread cause of congenital infection. The effects of various viral strains and viral loads on the infection outcome have been under debate.

Objectives: To determine the distribution of gN variants in HCMV strains isolated from children with congenital or postnatal infection and to establish the relationship between the viral genotype, the viral load, and the sequelae.

Analysis of the course and treatment of toxocariasis in children-a long-term observation.

Toxocariasis is a helminthozoonotic disease caused by ascarid larvae of Toxocara genus: Toxocara canis and Toxocara cati. In the reported study, the clinical course of toxocariasis and administered therapy were evaluated in 103 children. The majority of the children (68.

Lipid and carbohydrate parameters in children with chronic hepatitis C.

Purpose: HCV chronic infection still presents a very serious epidemiological and clinical problem. Apart from its cytopathic effect on liver parenchyma, its detrimental effect on lipid and carbohydrate metabolism has recently been emphasized. The aim of the study was to assess lipid and carbohydrate parameters in children with chronic HCV-related hepatitis.

Pneumonia caused by Mycoplasma pneumoniae and Chlamydophila pneumoniae in children - comparative analysis of clinical picture.

Purpose: The aim of the study was comparative analysis of clinical picture and prevalence of pneumonia caused by Mycoplasma pneumoniae and Chlamydophila pneumoniae in children.

Material And Methods: The study involved 332 children hospitalized in the 3rd Department of Paediatric, Polish Mother's Memorial Hospital - Research Institute, due to pneumonia caused by Mycoplasma pneumoniae - group I or Chlamydophila pneumonia - group II.

Results: Over 2003-2009 period there were 1870 children hospitalized due to pneumonia, of which in 332 (17.

[Underestimated problem of Chlamydia infections].

Microorganisms which belong to Chlamydophila sp and Chlamydia sp genus are intracellular pathogens with characteristic life cycle. In humans the most common infections are caused by Chlamydophila pneumoniae i Chlamydia trachomatis.These pathogens are mainly responsible for infections of upper respiratory tract, infections of urinary tract and conjunctivitis.

Lipid metabolism in children with chronic hepatitis C, A preliminary report.

Background/aims: The importance of lipid metabolism in the pathogenesis of chronic hepatitis C has been recognized in recent years. Bearing in mind remote sequelae of chronic hepatitis C in children, it seems reasonable to seek to determine potential risk factors for the development of cirrhosis and carcinogenesis. The aim of the study was to assess lipid metabolism in children with chronic HCV-related hepatitis.

Involvement of innate immunity in the pathogenesis of chronic hepatitis C in children.

The aim of the study was to assess the role of selected elements of innate immunity in the pathogenesis of chronic hepatitis C in children. The study comprised 20 children with chronic hepatitis C (group 1), nine healthy hepatitis C virus (HCV) seropositive children (group 2) and 18 healthy children (control group). We evaluated the expression of Toll-like receptor (TLR)2 and TLR4 on peripheral blood neutrophils, and generation of interleukin (IL)-8, IL-10, IL-12 and reactive oxygen species (ROS) by neutrophils.

[Specific features of Borrelia burgdorferi infection in children].

Clinical picture of Borrelia burgdorferi infection has been presented in 89 children from Lodz region. The analysis showed significant domination of cases with non specific symptoms (41.6%) as: fever or headache and cases with affected central and peripheral nervous system (30.

[Sustained virological response in children with chronic hepatitis C treated with interferon alpha and ribavirin].

Unlabelled: The aim of the study was to assess the effect of IFN-alpha and ribavirine combined therapy of chronic hepatitis C in children. The study comprised 37 children (22 boys and 15 girls) aged between 4 and 18 years (mean 12 years and 6 months) with chronic hepatitis C, diagnosed on the basis of serological, virological and histological criteria. The treatment included Intron A (Schering Plough) administered subcutaneously 3 M.

[Evaluation of the somatic development in children with chronic virus hepatitis].

The aim of the study was an evaluation of the somatic development of children with chronic hepatitis B, C and HBs antigen positive and finding the correlation between the nutrition status, the duration of disease and the aminotransferase activity. The somatic development was examined on the basis of body height and weight measurement and calculation of body mass index and Cole's index. Lowered body height and weight was observed in 25% of the children with chronic HCV infection.

[Carbohydrate metabolism in children with chronic hepatitis C].

Unlabelled: The importance of carbohydrate metabolism in the pathogenesis of chronic hepatitis C has been recognized in recent years. The aim of the study was to assess carbohydrate metabolism in children with chronic HCV-related hepatitis.

Material And Methods: The study was comprised of 16 children with chronic hepatitis C and 16 healthy controls.

[Neutrophil oxidative metabolism in children with chronic hepatitis C].

Unlabelled: A growing number of data suggest the role of reactive oxygen species (ROS) in the pathogenesis of chronic type c hepatitis. Activated neutrophils, macrophages and Kupffer cells are basic sources of ROS in the course of the inflammatory process in the liver. The aim of the study was to assess the metabolism of neutrophil oxygen in children with chronic hepatitis C.

[Results of 12-month lamivudine therapy of chronic hepatitis B in children].

Unlabelled: The aim of this study was to assess the results of 12-month lamivudine therapy in children with chronic hepatitis B.

Material And Methods: The study included 24 children (14 boys and 10 girls) aged from 7 to 18 years with chronic hepatitis B diagnosed according to current criteria. Lamivudine was administred in a daily dose of 3-5 mg/kg up to 100 mg for twelve months.

[Extrapulmonary complications of Mycoplasma pneumoniae infections].

Unlabelled: Mycoplasma pneumoniae is a common etiological factor in atypical pneumonia in children. The course of the disease is usually mild, but in some cases, especially in extrapulmonary localisation, it can pose significant diagnostic and therapeutic problems. The aim of this study was evaluation of extrapulmonary manifestations of Mycoplasma pneumoniae infections in children.

[Diagnostic problems with Borrelia burgdorferi infection in children].

Lyme borreliosis has become a serious diagnostic and therapeutic problem of modern medicine. The occurrence of the disease in Europe, and also in Poland, has recently increased. Diagnostic problems with Borrelia burgdorferi infection result from non-characteristic course of the disease in children, without the 1 st stage of the disease (erythema migrans), negative history forwards tick bite, lack of seasonal changes in occurrence and difficulties with interpretation of serological tests results.