Non-thermal plasma enhances growth and salinity tolerance of bok choy ( subsp. ) in hydroponic culture.

In this study, we aimed to examine the growth, physiological and biochemical status, and responses to salinity stress of bok choy ( subsp. ) cultivated in a hydroponic system with a plasma-treated solution. Plasma gas generated using a cylindrical dielectric barrier discharge or air (control) was injected into Hoagland nutrient solution once a week for different durations (0, 5, and 10 min).

RNA-Seq-Based Transcriptome Analysis of Nitric Oxide Scavenging Response in .

While the biological role of naturally occurring nitric oxide (NO) in filamentous fungi has been uncovered, the underlying molecular regulatory networks remain unclear. In this study, we conducted an analysis of transcriptome profiles to investigate the initial stages of understanding these NO regulatory networks in , a well-established model filamentous fungus. Utilizing RNA sequencing, differential gene expression screening, and various functional analyses, our findings revealed that the removal of intracellular NO resulted in the differential transcription of 424 genes.

Intracellular Nitric Oxide and cAMP Are Involved in Cellulolytic Enzyme Production in .

Although molecular regulation of cellulolytic enzyme production in filamentous fungi has been actively explored, the underlying signaling processes in fungal cells are still not clearly understood. In this study, the molecular signaling mechanism regulating cellulase production in was investigated. We found that the transcription and extracellular cellulolytic activity of four cellulolytic enzymes (, , , and ) increased in Avicel (microcrystalline cellulose) medium.

Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and Ca.

For the industrial-scale production of useful enzymes by microorganisms, technological development is required for overcoming a technical bottleneck represented by poor efficiency in the induction of enzyme gene expression and secretion. In this study, we evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve the production efficiency of cellulolytic enzymes in , a filamentous fungus. The total activity of cellulolytic enzymes and protein concentration were significantly increased (1.

Application of Non-Thermal Plasma to Fungal Resources.

In addition to being key pathogens in plants, animals, and humans, fungi are also valuable resources in agriculture, food, medicine, industry, and the environment. The elimination of pathogenic fungi and the functional enhancement of beneficial fungi have been the major topics investigated by researchers. Non-thermal plasma (NTP) is a potential tool to inactivate pathogenic and food-spoiling fungi and functionally enhance beneficial fungi.