Publications by authors named "Wiradee Sasikarn"
Article Synopsis
- The study aimed to evaluate how red blood cells (RBCs) can be coated with penicillin and to analyze the immune reactions in Thai patients' blood samples against these coated RBCs.
- It was found that penicillin-induced immunologic hemolytic anemia (IHA) may be linked to antibodies (IgG) that attack penicillin-coated RBCs, with evidence of some donors also having low levels of penicillin antibodies (often IgM).
- Results showed that out of 304 patients, 17 (5.59%) tested positive for antipenicillin using coated RBCs, while only 3 (1.22%) of 246 blood donors showed positive reactions; importantly, no links were found between patient characteristics
Anti-PP1Pk is produced by p individuals without prior red blood cell alloimmunization. This antibody can react over a wide thermal amplitude, has the potential to bind complement, and has caused hemolytic transfusion reaction, hemolytic disease of the fetus and newborn, and a high rate of spontaneous abortions. This report of two cases describes the genetic basis of p phenotype underlying anti-PP1Pk production and the development of a semi-nested polymerase chain reaction (PCR) assay for screening this observed mutation among Thai blood donors.
View Article and Find Full Text PDFBackground: Antibodies against human neutrophil antigens (HNAs) are involved in various clinical conditions including transfusion-related acute lung injury and auto/alloimmune neutropenia. We aimed to determine HNA-1, -3, -4, and -5 frequencies among southern Thais using multiplex PCR and to develop HNA-1 null detection.
Methods: Samples obtained from 427 southern Thai blood donors were genotyped HNA-1, -3, -4, and -5 using multiplex PCR and compared their allele frequencies with those previously reported in Thai populations.
Background: Different polymerase chain reaction (PCR) techniques for human neutrophil antigens (HNA) genotyping have been implemented to diagnose the clinical conditions of patients with alloimmune neutropenia, febrile non-hemolytic transfusion reactions, and transfusion-related acute lung injury and to provide effective HNAmatched granulocyte transfusions. The present study aimed to develop an in-house multiplex-PCR for HNA-1, -3, -4, and -5 genotyping in the Thai population.
Methods: Altogether, 500 DNA samples obtained from unrelated, healthy Thai blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, Thailand were included.