Localization of phospholipase C β3 in the major salivary glands of adult mice.

Phospholipase C (PLC)β has a role in saliva secretion by controlling intracellular Cavia its product, IP. The present study was attempted to localize PLCβ isoforms in mouse salivary glands in situ. A single major band was detected for PLCβ3 in immunoblots of the parotid and sublingual glands (PG, SLG), while no such band was seen in the submandibular gland (SMG).

Expression and localization of VIAAT in distal uriniferous tubular epithelium of mouse.

Vesicular inhibitory amino acid transporter (VIAAT) is a transmembrane transporter which is responsible for the storage of gamma-aminobutyric acid (GABA) or glycine in synaptic vesicles. According to recent studies, GABA is known to be expressed in the kidney. For clear understanding of the intra-renal GABA signaling, the localization of VIAAT was examined in the present study.

Co-localization of endogenous Arf6 and its activator EFA6D in the granular convoluted tubule cells of mouse submandibular glands under normal conditions and when stimulated by isoproterenol, noradrenaline and carbachol.

Objective: This study proposed to investigate the localization at light and electron microscopic levels of Arf6 and its activator EFA6D in the mouse submandibular gland (SMG) under normal conditions and when stimulated by adrenergic or cholinergic agonists.

Materials And Methods: SMGs of male adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and noradrenalin were used as adrenergics, while carbachol was used for the cholinergic stimulant.

Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol.

Objective: We wished to investigate the subcellular localization of CB1, a receptor for the endocannabinoids in mouse submandibular glands (SMGs) under normal conditions and when stimulated by adrenergic or cholinergic agonists.

Materials And Methods: SMGs of both male and female adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and carbachol were used as adrenergic and cholinergic stimulants, respectively.

Ultrastructural histometric evidence for expansion of the sustentacular cell envelope in response to hypersecretion of adrenal chromaffin cells in mice.

In our previous immuno-light microscopic study with an antibody for fatty acid binding protein of type 7 or brain type (FABP-7, B-FABP), the adrenomedullary sustentacular cells were revealed to have secondary processes that present faint immunostaining and an ill-defined sheet-like appearance, in addition to the well-recognized primary processes that present distinct immunostaining and a fibrous appearance. The secondary processes were regarded as corresponding to known ultrastructural profiles of sustentacular cells with a thickness of less than 0.2 µm (the resolution limit of light microscopy), and the processes were considered to be largely responsible for enveloping chromaffin cells.