Publications by authors named "Wioleta Gosk"
Adaptation of the AbsoluteIDQ p180 kit to the analysis of metabolites in the human aqueous humor.
J Chromatogr B Analyt Technol Biomed Life Sci
September 2023
The aim of this study was to use the commercial kit AbsoluteIDQ p180 (Biocrates) for the quantification of metabolites in aqueous humor (AH), as well as to determine the optimal volume of AH that is necessary to obtain reliable and reproducible results. Different volumes of AH (10 µl, 20 µl, and 30 µl) were tested. Of the 188 metabolites measurable with the Biocrates kit, 69 were detected in AH.
View Article and Find Full Text PDFThe aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic ( = 36) and nondiabetic ( = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ p180 kit.
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- * The study involved 25 participants divided into two groups: those with BAT (BAT+) and those without (BAT-), and assessed plasma endocannabinoid levels during cold exposure.
- * A significant decline in specific endocannabinoids was found in BAT+ individuals, suggesting that these molecules could be key targets for further obesity treatment strategies.
Metabolomics combined with machine learning methods (MLMs), is a powerful tool for searching novel diagnostic panels. This study was intended to use targeted plasma metabolomics and advanced MLMs to develop strategies for diagnosing brain tumors. Measurement of 188 metabolites was performed on plasma samples collected from 95 patients with gliomas (grade I-IV), 70 with meningioma, and 71 healthy individuals as a control group.
View Article and Find Full Text PDFInterocular comparison of the metabolomic signature of aqueous humor (AH) was performed. The aim of the study was to quantitatively evaluate the symmetry in concentrations of various metabolites belonging to different categories. The study included AH samples from 23 patients, 74.
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