Publications by authors named "Windy Ho"
Alzheimer's disease (AD) is the leading cause of dementia in older adults. Although AD progression is characterized by stereotyped accumulation of proteinopathies, the affected cellular populations remain understudied. Here we use multiomics, spatial genomics and reference atlases from the BRAIN Initiative to study middle temporal gyrus cell types in 84 donors with varying AD pathologies.
View Article and Find Full Text PDFWe present an enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits. Best-in-class vectors were curated for accessing major striatal neuron populations including medium spiny neurons (MSNs), direct and indirect pathway MSNs, as well as Sst-Chodl, Pvalb-Pthlh, and cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes of virus delivery, and with diverse transgene cargos.
View Article and Find Full Text PDFArticle Synopsis
- Researchers addressed the limited access to lower motor neurons (LMNs) in the mammalian spinal cord by creating single cell multiome datasets from mouse and macaque spinal cords to identify enhancers for different neuronal populations.* -
- They cloned identified enhancers into viral vectors and conducted functional tests in mice to screen for effective candidates, which were then validated in rats and macaques.* -
- This new toolkit for labeling LMNs and upper motor neurons (UMNs) can facilitate future research on cell function across species and contribute to potential therapies for neurodegenerative diseases in humans.*
Article Synopsis
- * This study analyzed over 600,000 single-cell transcriptomes from adult and developing mice to create a detailed classification of GABAergic neuron types, revealing a complex organization with numerous subclasses and clusters.
- * The research found that GABAergic neurons often migrate long distances and show variations in gene expression based on their spatial locations, with different stages of development leading to diversity in specific neuron types across various brain regions.
The mammalian brain consists of millions to billions of cells that are organized into many cell types with specific spatial distribution patterns and structural and functional properties. Here we report a comprehensive and high-resolution transcriptomic and spatial cell-type atlas for the whole adult mouse brain. The cell-type atlas was created by combining a single-cell RNA-sequencing (scRNA-seq) dataset of around 7 million cells profiled (approximately 4.
View Article and Find Full Text PDFAlzheimer's disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive and stereotyped accumulation of protein aggregates, but the underlying molecular and cellular mechanisms driving AD progression and vulnerable cell populations affected by disease remain coarsely understood. The current study harnesses single cell and spatial genomics tools and knowledge from the BRAIN Initiative Cell Census Network to understand the impact of disease progression on middle temporal gyrus cell types.
View Article and Find Full Text PDFArticle Synopsis
- The brain of a mouse has millions of different cells, and scientists want to make a complete list of these cell types to understand how the brain works.
- Researchers created a detailed map of these cells by studying around 7 million cells with a special technique that looks at their genes and how they are placed in the brain.
- They discovered that there are many different types of cells in the brain, with some areas being very unique, like the dorsal part having fewer but more distinct types, while the ventral part has many similar types.