Following the detection in Northern Ireland of bluetongue serotype-8 (BTV-8) PCR-positive calves born from PCR-negative but seropositive heifers imported from the Netherlands, an investigation was started to determine whether PCR-positive calves were born in the first quarter of 2008 in the Netherlands. Blood samples were collected on 43 dairy farms from 388 cow-calf combinations. All calves were at least 10-days-old and had been born after 1 January 2008.
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