Use of kinetic plots for the optimization of the separation time in ultra-high-pressure LC.

The kinetic-plot approach, in which experimental t(0) and N-values are extrapolated to the performance at maximum system pressure by increasing the column length, was validated by coupling 250×3 mm columns packed with 3 μm particles. The extra-column volume introduced by coupling columns could be neglected with respect to the peak volumes. Plate numbers of up to 132,000 were experimentally achieved by coupling four columns.

A protocol for designing comprehensive two-dimensional liquid chromatography separation systems.

In this paper a protocol is proposed for establishing suitable column dimensions (length and diameters), particle sizes, flow rates, and second-dimension injection volumes (i.e. loop sizes) in comprehensive two-dimensional liquid chromatography (LC x LC).

Comparison of the efficiency of microparticulate and monolithic capillary columns.

A comparison is made between the efficiency of microparticulate capillary columns and silica and polymer-based monolithic capillary columns in the pressure-driven (high-performance liquid chromatography) and electro-driven (capillary electrochromatography) modes. With packed capillary columns similar plate heights are possible as with conventional packed columns. However, a large variation is observed in the plate heights for individual columns.