Abca4, mutated in Stargardt disease, is required for structural integrity of cone outer segments.

Stargardt disease (STGD), the leading cause of inherited childhood blindness, is primarily caused by mutations in the ABCA4 gene; yet, the underlying mechanisms of photoreceptor degeneration remain elusive, partly due to limitations in existing animal disease models. To expand our understanding, we mutated the human ABCA4 paralogues abca4a and abca4b in zebrafish, which has a cone-rich retina. Our study unveiled striking dysmorphology and elongation of cone outer segments (COS) in abca4a;abca4b double mutants, alongside reduced phagocytosis by the retinal pigmented epithelium (RPE).

A mixed methods examination of a text message-based mHealth media literacy intervention for U.S. college students amid the COVID-19 pandemic.

: During the COVID-19 pandemic, college students were navigating confusing and often conflicting information on social media. Media literacy can help people interpret information online. We developed and tested a text-message media literacy intervention designed for college students.

A proof-of-concept study examining a health communication intervention to reduce cannabis misuse among college students.

Objective: A growing number of U.S. states have legalized cannabis for people 21 and older, but cannabis use and misuse can negatively impact college students' health and academic achievements.

Detectability of runs of homozygosity is influenced by analysis parameters and population-specific demographic history.

Wild populations are increasingly threatened by human-mediated climate change and land use changes. As populations decline, the probability of inbreeding increases, along with the potential for negative effects on individual fitness. Detecting and characterizing runs of homozygosity (ROHs) is a popular strategy for assessing the extent of individual inbreeding present in a population and can also shed light on the genetic mechanisms contributing to inbreeding depression.

Public responses and parasocial relationships following senator John Fetterman's depression disclosure.

Research has found that when a public figure discloses an illness, it can motivate members of the public to reconsider their own health behaviors, particularly when they have a parasocial relationship with the public figure. When the public figure is a politician, it is possible that partisan differences may also influence emotional, attentional, and behavioral responses to health news. We empirically examined public responses to Democrat John Fetterman's disclosure of his treatment for depression shortly after he was inducted into the United States Senate as the junior senator from Pennsylvania in 2023.

Conceptualization of Firearm-Related Terms Among Rural Adolescents: Definitions Matter.

Rural adolescents are at risk for firearm-related injury and death. In response, professional organizations have called for communication between adolescents, parents, and providers about firearms. A shared understanding of firearms between providers and families can facilitate effectiveness of health interventions.

A quantitative and systematic analysis of Anopheles stephensi bionomics and control approaches.

Anopheles stephensi is a mosquito endemic to South Asia and the Arabian Peninsula that has recently been detected in eight African countries, posing a significant threat to global malaria control efforts. A challenge with An. stephensi is that it requires unique surveillance and control tools when compared to other malaria vectors.

Impact of morphology and oxygen vacancy content in Ni, Fe co-doped ceria for efficient electrocatalyst based water splitting.

Designing a highly efficient, low-cost, sustainable electrocatalyst for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) through water splitting is a current challenge for renewable energy technologies. This work presents a modified sol-gel route to prepare metal-ion(s) doped cerium oxide nanostructures as an efficient electrocatalyst for overall water splitting. Nickle (Ni) and iron (Fe) co-doping impacts the morphology in cerium oxide resulting in 5 nm nanoparticles with a mesoporous-like microstructure.

Genomic Diversity as a Key Conservation Criterion: Proof-of-Concept From Mammalian Whole-Genome Resequencing Data.

Many international, national, state, and local organizations prioritize the ranking of threatened and endangered species to help direct conservation efforts. For example, the International Union for Conservation of Nature (IUCN) assesses the Green Status of species and publishes the influential Red List of threatened species. Unfortunately, such conservation yardsticks do not explicitly consider genetic or genomic diversity (GD), even though GD is positively associated with contemporary evolutionary fitness, individual viability, and with future evolutionary potential.

A methodological pipeline for the preclinical evaluation of novel topical agents for the treatment of CRS.

Novel topical therapeutics require extensive pre-clinical testing to assess efficacy and safety. Antibiofilm or immunosuppressant agents can utilize ex vivo models to measure ciliotoxicity. Agents that are found to be effective and non-toxic ex vivo warrant further investigation in vivo.

Impact of isotype on the mechanism of action of agonist anti-OX40 antibodies in cancer: implications for therapeutic combinations.

Background: OX40 has been widely studied as a target for immunotherapy with agonist antibodies taken forward into clinical trials for cancer where they are yet to show substantial efficacy. Here, we investigated potential mechanisms of action of anti-mouse (m) OX40 and anti-human (h) OX40 antibodies, including a clinically relevant monoclonal antibody (mAb) (GSK3174998) and evaluated how isotype can alter those mechanisms with the aim to develop improved antibodies for use in rational combination treatments for cancer.

Methods: Anti-mOX40 and anti-hOX40 mAbs were evaluated in a number of in vivo models, including an OT-I adoptive transfer immunization model in hOX40 knock-in (KI) mice and syngeneic tumor models.

Habitat remediation followed by managed connectivity reduces unwanted changes in evolutionary trajectory of high extirpation risk populations.

As we continue to convert green spaces into roadways and buildings, connectivity between populations and biodiversity will continue to decline. In threatened and endangered species, this trend is particularly concerning because the cessation of immigration can cause increased inbreeding and loss of genetic diversity, leading to lower adaptability and higher extirpation probabilities in these populations. Unfortunately, monitoring changes in genetic diversity from management actions such as assisted migration and predicting the extent of introduced genetic variation that is needed to prevent extirpation is difficult and costly in situ.

Rapid vertebrate speciation via isolation, bottlenecks, and drift.

Speciation is often driven by selective processes like those associated with viability, mate choice, or local adaptation, and "speciation genes" have been identified in many eukaryotic lineages. In contrast, neutral processes are rarely considered as the primary drivers of speciation, especially over short evolutionary timeframes. Here, we describe a rapid vertebrate speciation event driven primarily by genetic drift.

Assessing and managing the risk of Aedes mosquito introductions via the global maritime trade network.

The global shipping network (GSN) has been suggested as a pathway for the establishment and reintroduction of Aedes aegypti and Aedes albopictus primarily via the tire trade. We used historical maritime movement data in combination with an agent-based model to understand invasion risk in the United States Gulf Coast and how the risk of these invasions could be reduced. We found a strong correlation between the total number of cargo ship arrivals at each port and likelihood of arrival by both Ae.

The Impact of Celebrity and Influencer Illness Disclosures.

When celebrities, political figures, influencers, or anyone with a large following publicly disclose an illness or die, the news becomes a de facto public health campaign. Until health communicators began studying such disclosures and the effects of the following waves of media coverage, however, it was not known to what extent these events impacted the public. A growing body of research has empirically documented these events and examined the factors that predict which types of audiences are most affected and why.

#LoveYourBody: An Experimental Test of the Effects of Objectification and Body Appreciation Content on Instagram Fitness and Health Posts Among Young Women.

Fitspiration content on Instagram often includes images in which women are objectified but may also include captions that aim to positively motivate viewers, such as through messaging about body appreciation. Viewing objectifying content in fitspiration posts may be problematic for young women's mental health, but it's unclear if positive messaging may help to alleviate some of these effects. In this study, we conducted a 2 × 2 online experiment assessing the effects of body appreciation and objectification content present in Instagram fitness posts by influencers on young women ( = 200).

Remotely sensed environmental measurements detect decoupled processes driving population dynamics at contrasting scales.

The increasing availability of satellite imagery has supported a rapid expansion in forward-looking studies seeking to track and predict how climate change will influence wild population dynamics. However, these data can also be used in retrospect to provide additional context for historical data in the absence of contemporaneous environmental measurements. We used 167 Landsat-5 Thematic Mapper (TM) images spanning 13 years to identify environmental drivers of fitness and population size in a well-characterized population of banner-tailed kangaroo rats () in the southwestern United States.

Fructose induced KHK-C can increase ER stress independent of its effect on lipogenesis to drive liver disease in diet-induced and genetic models of NAFLD.

Non-alcoholic fatty liver disease (NAFLD) is a liver manifestation of metabolic syndrome, and is estimated to affect one billion individuals worldwide. An increased intake of a high-fat diet (HFD) and sugar-sweetened beverages are risk-factors for NAFLD development, but how their combined intake promotes progression to a more severe form of liver injury is unknown. Here we show that fructose metabolism via ketohexokinase (KHK) C isoform leads to unresolved endoplasmic reticulum (ER) stress when coupled with a HFD intake.

Examining science and media literacy health communication messages to reduce intentions to use cannabis while pregnant.

Objective: Although use of cannabis during pregnancy can be detrimental to the fetus, use of cannabis during pregnancy has increased. Pregnant people are often exposed to incorrect information about cannabis use during pregnancy online and have expressed a desire for additional information about the effects of using cannabis while pregnant. We wanted to design and test a brief intervention promoting media literacy and science literacy and assess whether exposure would reduce intentions to use cannabis during pregnancy.

An Exploratory Content Analysis of the Use of Health Communication Strategies and Presence of Objectification in Fitness Influencer Social Media Posts.

Health and fitness content intended to inspire people to live healthy lives (e.g. "fitspiration") has been linked to negative body image among girls and young women.

The impact of sexual scripts in brand-generated cannabis social media posts on sex-related cannabis expectancies: Does body appreciation moderate effects?

Introduction: Cannabis messaging on digital media may include sexualised portrayals. We examined whether exposure to and perceptions of cannabis posts that included sexual objectification impacted two types of sex-related cannabis expectancies-sexual risk and sexual enhancement-and whether body appreciation moderated these relationships.

Methods: We conducted an online experiment with college students in Washington state.

Metabolically Stable Anomeric Linkages Containing GalNAc-siRNA Conjugates: An Interplay among ASGPR, Glycosidase, and RISC Pathways.

Conjugation of synthetic triantennary -acetyl-d-galactosamine (GalNAc) to small interfering RNA (siRNA) mediates binding to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes, facilitating liver-specific uptake and siRNA-mediated gene silencing. The natural β-glycosidic bond of the GalNAc ligand is rapidly cleaved by glycosidases in vivo. Novel GalNAc ligands with -, and -glycosides with both α- and β-anomeric linkages, -glycosides with β-anomeric linkage, and the glycoside with α-anomeric linkage were synthesized and conjugated to siRNA either on-column during siRNA synthesis or through a high-throughput, post-synthetic method.

Exposure to Pro and Anti-Cannabis Social Media Messages and Teens' and College Students' Intentions to Use Cannabis.

Content analyses have documented that posts about cannabis are increasingly common on social media. The relationship between the cannabis-related content to which teens and college students are exposed on social media and how such content may be associated with intentions to use and use of cannabis is less known, however. We conducted an online survey with teens ( = 350) who lived in Washington state using online survey panel participants in June 2018 and with college students ( = 966) in a Washington state-wide university system in February and March 2019.

Application of the Policy Regime Framework to understand COVID-19 policy response in the Southeast U.S.: How RAPID research can provide lessons learned after a public health crisis.

Quick-response research during a time of crisis is important because time-sensitive findings can inform urgent decision-making, even with limited research budgets. This research, a National Science Foundation-funded Rapid Response Research (RAPID), explores the United States (U.S.