Fluorimetric analysis of hydrogen peroxide with automated measurement.

Unlabelled: In the pathophysiology of chronic obstructive pulmonary disease (COPD) oxidative stress plays an important role, which can be determined by measuring hydrogen peroxide. Hydrogen peroxide can be measured fluorimetrically in exhaled breath condensate (EBC), however, not standardized. The objective of this study was to investigate the sensitivity and reproducibility of measuring the hydrogen peroxide concentration in EBC of COPD patients using an automated flow injection device with varying flow rates and measurements.