Paediatric resuscitation in regional Queensland: A simulation informed biopsy of current system challenges.

Objectives: An outreach education service, Simulation Training Optimising Resuscitation for Kids (STORK) identified opportunities to use their course (Optimus PRIME) to understand and help optimise regional paediatric resuscitation. Our objective was to document challenges faced by healthcare providers in rural, remote, and regional (RRR) health centres in Queensland during a paediatric resuscitation course and ensure information reached stakeholders.

Methods: Using in situ simulation, participants were prompted to identify real-life challenges during paediatric resuscitation.

Menstrual product use patterns in a large digital cohort in the United States: Variations by sociodemographic, health, and menstrual characteristics.

Background: Using menstrual products is a part of managing menstrual bleeding. Products use may represent individual, social, and economic influences. A few studies on menstrual product use from specific regions in the United States (US) reported differences in use across demographic factors like age and race and ethnicity.

EZH2 coordinates memory B-cell programming and recall responses.

Antigen-experienced memory B-cells (MBC) are endowed with enhanced functional properties compared to naïve B cells and play an important role in the humoral response. However, the epigenetic enzymes and programs that govern their rapid differentiation are incompletely understood. Here, the role of the histone H3 lysine 27 methyltransferase EZH2 in the formation of MBC in response to an influenza infection was determined in Mus musculus.

Addressing Microaggressions-Building the Foundation for Nurse Leaders.

This article examines the methodology and outcomes associated with the development of a synchronous, virtual educational activity designed for nurse leaders to enhance their ability to identify and respond to microaggressions. The engagement strategies and techniques for fostering empathy and awareness discussed can be applied widely. However, they are particularly impactful for activities related to diversity, equity, and inclusion, such as this course that focuses on recognizing and effectively managing microaggressions among nursing leaders.

New ways to use imaging data in cardiovascular research: survey of opinions on federated learning and synthetic data.

Aims: Federated learning and the creation of synthetic data are emerging tools, which may enhance the use of imaging data in cardiovascular research. This study sought to understand the perspectives of cardiovascular imaging researchers on the potential benefits and challenges associated with these technologies.

Methods And Results: The British Heart Foundation Data Science Centre conducted a series of online surveys and a virtual workshop to gather insights from stakeholders involved in cardiovascular imaging research about federated learning and synthetic data generation.

How to Harness Education Fellows to Optimise Clinical Placement Capacity.

There is a global need to increase our medical workforce to meet the demands of changing population demography and increased complex comorbidity. Recruitment to medicine, nursing and other healthcare professions must significantly increase [1]; however, clinical placement saturation is becoming a rate-limiting step to substantial increase in student numbers. This article will consider how clinical placement providers can deploy education fellows (EFs) to innovatively create capacity whilst enhancing undergraduate clinical placement quality.

An Evaluation of Time Spent Completing Electronically Collected Patient-Reported Outcomes in Clinical Trials.

Objectives: Patient-reported outcomes (PROs) are important measures of efficacy in the context of clinical trials but are sometimes identified as time and resource intensive to study participants and site personnel. The objective of this research was to evaluate the amount of time that participants spend completing PROs via an electronic device in phase 2 and 3 clinical trials across several disease areas.

Methods: The electronic Clinical Outcome Assessment (eCOA) data were obtained from Johnson & Johnson clinical trials across various disease areas from 2016 to 2023.

Guidelines for Diagnosis and Management of Idiopathic Normal Pressure Hydrocephalus.

In 1965, Hakim and Adams described 3 patients with normal pressure hydrocephalus who responded to treatment with a ventriculoatrial shunt. Afterward the adoption of shunt treatment without clear diagnostic criteria and surgical techniques resulted in poor outcomes with significant complications. The clinical practice guidelines for the diagnosis and treatment of idiopathic normal pressure hydrocephalus were first published by the Japanese Neurosurgical Society in 2004 and the international guidelines were published in 2005.

Adult Hydrocephalus Clinical Subtypes.

Adult hydrocephalus comprises 4 pragmatic categories: (1) transition; (2) unrecognized congenital; (3) acquired; and (4) suspected idiopathic normal pressure hydrocephalus. Each of these groups has unique clinical presentations and care needs that require the involvement of adult neurosurgeons for diagnosis and treatment. Patients in all 4 of these categories benefit from longitudinal care for monitoring of their symptoms or assessing response to treatment.

Patterns of Cannabis Use among Women With HIV in the United States.

Cannabis legalization in the United States changed cannabis product potency and consumption modalities. Women with HIV are an aging population with prevalent cannabis use twice that of national estimates. Because cannabis use may confer both health benefits and risks, it is important to understand cannabis use patterns among women with HIV.

Medical vs MicroCT based Finite Element Analysis : Exploring the Influence of Bone Heterogeneity and Bone Geometry.

A comprehensive investigation into the direct comparison of medical-CT and μCT-based FEA regarding the impact of bone heterogeneity and geometry is lacking. Therefore, in this study, the impact of bone heterogeneity and bone geometry in FEA for the evaluation of uncemented prostheses is demonstrated. The comparison between three different material models and two different CT scan technologies (medical-CT and μCT) was conducted by evaluating the FEA models at a given ROI under the same loading conditions.

Absolute quantification of the living skin microbiome overcomes relic-DNA bias and reveals specific patterns across volunteers.

Background: As the first line of defense against external pathogens, the skin and its resident microbiota are responsible for protection and eubiosis. Innovations in DNA sequencing have significantly increased our knowledge of the skin microbiome. However, current characterizations do not discriminate between DNA from live cells and remnant DNA from dead organisms (relic DNA), resulting in a combined readout of all microorganisms that were and are currently present on the skin rather than the actual living population of the microbiome.

Socioeconomic status, cardiovascular risk factors and coronary atherosclerosis: a SCOT-HEART trial analysis.

Aims: To investigate the relationship between socioeconomic status, plaque burden on coronary computed tomography angiography (CCTA), management and outcomes.

Methods: In a post-hoc analysis of a multicentre randomised control trial, we assessed associations between socioeconomic status and qualitative (stenosis, adverse plaque characteristics) and quantitative (total plaque, calcified plaque, non-calcified and low attenuation) CCTA plaque features and examined the interaction of socioeconomic status on cardiovascular outcomes.

Results: Socioeconomic status was available in 3948 participants of whom 1989 were randomised to CCTA and 1629 scans were suitable and available for quantitative plaque analysis.

Cellular pharmacology of tenofovir alafenamide and emtricitabine in neutrophils and platelets in people with and without HIV.

Background: Previous studies have primarily focused on nucleos(t)ide reverse transcriptase inhibitor pharmacology in peripheral blood mononuclear cells (PBMCs) and erythrocytes via dried blood spots (DBS), but not other major blood cells.

Objectives: Our objectives were to describe and compare the concentrations of tenofovir-diphosphate (TFV-DP) and emtricitabine-triphosphate (FTC-TP) in DBS, PBMCs, neutrophils, and platelets in people with HIV (PWH) and people without HIV (PWOH).

Methods: DBS, PBMCs, neutrophils, and platelets were isolated from whole blood drawn from PWH and PWOH receiving tenofovir alafenamide and emtricitabine.

CRISPR/Cas9 Methods for Identification and Validation of Genes Regulating BCR-Mediated Antigen Uptake.

Genome-wide CRISPR screens are a powerful tool to interrogate and identify gene function in a wide variety of applications and cell types. CRISPR-Cas9 technology using pooled CRISPR single guide RNA (sgRNA) libraries enables genetic editing in bulk in a large population of cells of interest. After selection of gene-edited cells, phenotyping effects can be evaluated by quantifying abundance (over- or under-representation) of individual sgRNAs using DNA sequencing.

Arabidopsis research in 2030: Translating the computable plant.

Plants are essential for human survival. Over the past three decades, work with the reference plant Arabidopsis thaliana has significantly advanced plant biology research. One key event was the sequencing of its genome 25 years ago, which fostered many subsequent research technologies and datasets.

T-cell immune checkpoint inhibition plus hypomethylation for locally advanced HER2-negative breast cancer: a phase 2 neoadjuvant window trial of decitabine and pembrolizumab followed by standard neoadjuvant chemotherapy.

Background: Higher levels of tumor-infiltrating lymphocytes (TILs) in breast cancers are associated with increased likelihood of pathologic complete response (pCR) to chemotherapy. DNA methyltransferase inhibitors (DNMTi) can augment immune responses to cancers, decreasing myeloid-derived suppressor cells (MDSCs) and increasing T lymphocyte responsiveness. We have shown that the DNMTi decitabine augments the effectiveness of immunotherapy using murine triple-negative breast cancer (TNBC) models.

Recovery of cardiovascular testing in Asia during the COVID-19 pandemic: findings from the INCAPS COVID 2 study.

Background: Understanding pandemic-related reductions and subsequent recovery of cardiovascular testing in Asia is important for guiding regional public health efforts.

Objectives: This study sought to evaluate the recovery of cardiovascular testing in Asia 1 year into the COVID-19 pandemic.

Methods: In this subanalysis of a worldwide survey on the impact of COVID-19 on cardiovascular diagnostic care in April 2020 and April 2021, recovery of testing volume in Asia was compared among subregions, World Bank income groups and imaging modalities.

Measurement of the Branching Fraction Ratios R(D^{+}) and R(D^{*+}) Using Muonic τ Decays.

The branching fraction ratios of B[over ¯]^{0}→D^{+}τ^{-}ν[over ¯]_{τ} and B[over ¯]^{0}→D^{*+}τ^{-}ν[over ¯]_{τ} decays are measured with respect to their muonic counterparts, using a data sample corresponding to an integrated luminosity of 2.0  fb^{-1} collected by the LHCb experiment in proton-proton collisions at sqrt[s]=13  TeV. The reconstructed final states are formed by combining D^{+} mesons with τ^{-}→μ^{-}ν[over ¯]_{μ}ν_{τ} candidates, where the D^{+} is reconstructed via the D^{+}→K^{-}π^{+}π^{+} decay.

Supports and Barriers to Inclusive Workplaces for LGBTQIA+ Autistic Adults in the United States.

Background: Autistic people and lesbian, gay, bisexual, transgender, queer, intersex, asexual, and people of other sexuality or gender minorities (LGBTQIA+) experience worse unemployment rates than allistic or cisgender, straight people. Yet, there is limited research to understand the employment experiences of people with both autistic and LGBTQIA+ identities. Therefore, in this study, we aim to describe the barriers and supports for inclusion in the workplace for autistic LGBTQIA+ adults.

Effective preconcentration of volatile per- and polyfluoroalkyl substances from gas and aqueous phase via solid phase microextraction.

Background: Per- and polyfluoroalkyl substances (PFAS) are synthetic fluorinated chemicals of increasing global concern due to their persistence, toxicity, and widespread presence in the environment. Neutral and volatile PFAS, used in firefighting foams and non-stick coatings, are precursors of perfluorinated acids such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) and necessitate effective preconcentration methods for isolation from aqueous and gaseous phases.

Results: In this work, a robust quantitative method was rigorously optimized and validated to achieve effective preconcentration and quantification of neutral volatile PFAS, including fluorotelomer alcohols (FTOHs), perfluorooctanesulfonamides (FOSAs), and a perfluorooctanesulfonamido-ethanol (FOSE).

2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes: A Report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines.

Aim: The "2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes" incorporates new evidence since the "2013 ACCF/AHA Guideline for the Management of ST-Elevation Myocardial Infarction" and the corresponding "2014 AHA/ACC Guideline for the Management of Patients With Non-ST-Elevation Acute Coronary Syndromes" and the "2015 ACC/AHA/SCAI Focused Update on Primary Percutaneous Coronary Intervention for Patients With ST-Elevation Myocardial Infarction." The "2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes" and the "2021 ACC/AHA/SCAI Guideline for Coronary Artery Revascularization" retire and replace, respectively, the "2016 ACC/AHA Guideline Focused Update on Duration of Dual Antiplatelet Therapy in Patients With Coronary Artery Disease."

Methods: A comprehensive literature search was conducted from July 2023 to April 2024.

2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes: A Report of the American College of Cardiology/American Heart Association Joint Committee on Clinical Practice Guidelines.

Aim: The "2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes" incorporates new evidence since the "2013 ACCF/AHA Guideline for the Management of ST-Elevation Myocardial Infarction" and the corresponding "2014 AHA/ACC Guideline for the Management of Patients With Non-ST-Elevation Acute Coronary Syndromes" and the "2015 ACC/AHA/SCAI Focused Update on Primary Percutaneous Coronary Intervention for Patients With ST-Elevation Myocardial Infarction." The "2025 ACC/AHA/ACEP/NAEMSP/SCAI Guideline for the Management of Patients With Acute Coronary Syndromes" and the "2021 ACC/AHA/SCAI Guideline for Coronary Artery Revascularization" retire and replace, respectively, the "2016 ACC/AHA Guideline Focused Update on Duration of Dual Antiplatelet Therapy in Patients With Coronary Artery Disease."

Methods: A comprehensive literature search was conducted from July 2023 to April 2024.

Comparison of Sprague Dawley with Long Evans rats on a battery of widely used neurobehavioral tests.

Genetic knockout (KO) models are valuable tools for understanding biological functions and disease mechanisms. KO models in rats have fallen behind those in mice which limits advances in areas where rats have advantages because they can perform more complex cognitive tasks. Two widely used rat strains are Sprague Dawley (SD) and Long Evans (LE).