Publications by authors named "William T Kaufhold"
Article Synopsis
- A variety of biological agents can disrupt lipid membranes, including amyloid aggregates, antimicrobial peptides, and venom compounds, which are important for both diseases and therapeutic technologies.
- The text introduces synthetic, DNA-based particles that can disrupt lipid membranes, designed with a core for adhesion and a protective outer layer that can be removed to enhance membrane interaction.
- These particles can increase the permeability of lipid vesicles, leading to vesicle collapse, and can also form DNA aggregates that can trap bacteria, mimicking the immune response of trapping pathogens.
DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals.
View Article and Find Full Text PDFInteractions between biomolecules such as proteins underlie most cellular processes. It is crucial to visualize these molecular-interaction complexes directly within the cell, to show precisely where these interactions occur and thus improve our understanding of cellular regulation. Currently available proximity-sensitive assays for in situ imaging of such interactions produce diffraction-limited signals and therefore preclude information on the nanometer-scale distribution of interaction complexes.
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