Publications by authors named "William S Trout"
Article Synopsis
- The study introduces ligand-directed catalysts that utilize red light to enable bioorthogonal chemistry within live cells, highlighting the ability to control chemical reactions through light activation.
- Silarhodamine dyes are repurposed as photocatalysts, demonstrating high compatibility with cells and minimal byproducts, while other conjugates are used to target specific cell components like the nucleus and microtubules.
- The research shows that a novel photocage can release chemical compounds within cells, effectively promoting microtubule destabilization, which can be monitored in real-time using confocal microscopy.
Article Synopsis
- Researchers are exploring silicon-Rhodamine compounds (SiRs) as photocatalysts that use 660 nm light to enable bioorthogonal chemical reactions in biological systems, addressing limitations of traditional singlet oxygen sensitization.
- SiR derivatives, particularly Janelia Fluor-SiR dyes, effectively catalyze oxidation reactions without degrading target proteins, demonstrating high stability and potential for chemical modifications.
- This method allows for photoinducible hydrogel formation, applicable in 3D cell cultures and live mice, offering a cytocompatible approach with potential for broad applications in biological research.
Rapid bioorthogonal reactivity can be induced by controllable, catalytic stimuli using air as the oxidant. Methylene blue (4 μM) irradiated with red light (660 nm) catalyzes the rapid oxidation of a dihydrotetrazine to a tetrazine thereby turning on reactivity toward trans-cyclooctene dienophiles. Alternately, the aerial oxidation of dihydrotetrazines can be efficiently catalyzed by nanomolar levels of horseradish peroxidase under peroxide-free conditions.
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