Management of herbicide-tolerant oilseed rape in Europe: a case study on minimizing vertical gene flow.

The potential commercialization of genetically modified herbicide-tolerant (GMHT) oilseed rape in Europe raises various concerns about their potential environmental and agronomic impacts, especially those associated with the escape of transgenes. Pollen of oilseed rape can be dispersed in space, resulting in the fertilization of sympatric compatible wild relatives (e.g.

Phylogeny of efflux-mediated tetracycline resistance genes and related proteins revisited.

A SRS search in the GenBank/EMBL databases for entire genes encoding efflux-mediated resistance allocated to a recognized tetracycline determinant revealed the existence of at least 87 genes. DNA-based and protein sequence analyses of representatives from the different efflux-mediated tetracycline determinant groups were performed and allowed us to propose a revision of the current grouping on the basis of our new evolutionary trees. On the other hand, similarity, topology, and hydropathy analyses of some representatives from 12-transmembrane segments (TMS) and 14-TMS proteins lead us to perform meaningful sequence alignments of recognized or putative 12-TMS and 14-TMS proteins truncated to their first 200 amino acids (alpha-domain of the protein).