Publications by authors named "William M Bourne"
Purpose: Measuring patient-reported visual disability in Fuchs' endothelial corneal dystrophy (FECD) may be helpful in determining when to intervene and for understanding the outcomes of intervention. In this study, we aimed to validate a new patient-reported visual disability questionnaire, the Visual Function and Corneal Health Status (V-FUCHS) instrument, in FECD before and after endothelial keratoplasty (EK).
Design: Cross-sectional study with instrument readministration at 6 weeks.
Purpose: To assess the relationship between graft thickness and best-corrected visual acuity (BCVA) after Descemet stripping endothelial keratoplasty (DSEK).
Design: Systematic review and meta-analysis.
Methods: PubMed, EMBASE, Web of Science, and conference abstracts were searched for studies published up to October 2015 with standard systematic review methodology.
Purpose: To determine the effects of keratocyte loss on optical properties and vision after laser in situ keratomileusis (LASIK) with the flap created with a femtosecond laser or a mechanical microkeratome.
Design: Randomized clinical paired-eye study.
Methods: Both eyes of 21 patients received LASIK for myopia or myopic astigmatism.
Purpose: To assess vision-related quality of life in Fuchs' dystrophy and changes in vision-related quality of life after 3 types of keratoplasty (penetrating keratoplasty [PK], deep lamellar endothelial keratoplasty [DLEK], and Descemet stripping endothelial keratoplasty [DSEK]).
Design: Prospective, observational case series.
Participants: Sixty-three subjects with Fuchs' endothelial dystrophy: 12 subjects (12 eyes) received PK, 11 subjects (11 eyes) received DLEK, and 40 subjects (40 eyes) received DSEK.
Purpose: To compare corneal endothelial cell density (ECD) and morphology between flap creation with a femtosecond laser and flap creation with a mechanical microkeratome 5 years after laser in situ keratomileusis (LASIK).
Setting: Mayo Clinic, Rochester, Minnesota, USA.
Design: Prospective randomized masked paired-eye study.
Purpose: To determine corneal sensitivity and evaluate corneal nerves before and after keratoplasty for Fuchs endothelial dystrophy.
Methods: Central corneal sensitivity, measured by using a Cochet-Bonnet esthesiometer in 69 eyes before and after different keratoplasty procedures for Fuchs dystrophy, was compared with that of 35 age-matched normal corneas. Corneal nerves were qualitatively examined by confocal microscopy in 42 eyes before and after Descemet stripping endothelial keratoplasty (DSEK).
Objective: To compare changes in subbasal nerve density and corneal sensitivity after laser in situ keratomileusis (LASIK) with the flap created by a femtosecond laser (bladeless) vs a mechanical microkeratome.
Design: In a randomized paired-eye study, 21 patients received myopic LASIK with the flap created by a femtosecond laser in one eye and by a mechanical microkeratome in the fellow eye. Eyes were examined before and at 1, 3, 6, 12, and 36 months after LASIK.
Objective: To review the history of the growth in knowledge about the corneal endothelium.
Methods: Publications concerning the corneal endothelium were reviewed.
Results: Highpoints in the growth of knowledge about the corneal endothelium include discovery of barrier and pump functions, specular microscopy, reduction in surgical trauma, corneal preservation, and future advances.
Standardization of corneal haze measurement in confocal microscopy.
Invest Ophthalmol Vis Sci
November 2010
Purpose: Corneal stromal haze cannot be compared in longitudinal studies or across laboratories without standardization. In this study, a method was devised of standardizing image brightness in confocal microscopy of the cornea.
Methods: Thirty-six normal corneas of 18 untreated volunteers and 35 corneas of 18 patients 3 years after LASIK were examined by slit scanning confocal microscopy.
Purpose: The center method of corneal endothelial cell analysis is rapid but excludes the outermost digitized cells of a contiguous group from analysis; the flex-center method (Konan, Inc) is a modification that includes analysis of the outermost cells, which is advantageous in images with few cells. In this study, we examined agreement among the flex-center, center, and corner (standard) methods of endothelial analysis.
Methods: Identical cells in endothelial images of 10 normal corneas and 10 corneas after penetrating keratoplasty (PK) were analyzed by each method.
Objective: To determine donor risk factors for graft failure and late endothelial failure (LEF) 20 years after penetrating keratoplasty (PK).
Methods: Prospective, observational study of 500 consecutive PKs performed by one surgeon. After excluding eyes undergoing second grafts, fellow eyes of bilateral cases, and patients who withdrew research authorization, 388 eyes (388 patients) were available for analysis.
Purpose: To compare corneal high-order aberrations and visual acuity after laser in situ keratomileusis (LASIK) with the flap created by a femtosecond laser (bladeless) to LASIK with the flap created by a mechanical microkeratome.
Design: Prospective, randomized, paired-eye study.
Methods: Fellow eyes of 21 patients with myopia or myopic astigmatism were randomized by ocular dominance.
Corneal endothelial cell loss 9 years after excimer laser keratorefractive surgery.
Arch Ophthalmol
November 2009
Objective: To determine the long-term changes in the corneal endothelium after laser in situ keratomileusis (LASIK) and photorefractive keratectomy (PRK).
Methods: Twenty-nine eyes (16 patients) received myopic LASIK or PRK, with intended correction to emmetropia. Central endothelial photographs were taken before and 9 years after surgery and were analyzed by the same masked investigator after appropriate calibration for magnification.
Automated assessment of keratocyte density in stromal images from the ConfoScan 4 confocal microscope.
Invest Ophthalmol Vis Sci
April 2010
Purpose. To develop a program to determine cell densities in images from the ConfoScan 4 (Nidek, Inc., Freemont, CA) confocal microscope and compare the densities with those determined in images obtained by the Tandem Scanning confocal microscope (Tandem Scanning Corp.
View Article and Find Full Text PDFPurpose: To evaluate high-order aberrations (HOA) induced by the anterior corneal surface after deep lamellar endothelial keratoplasty (DLEK) and penetrating keratoplasty (PK).
Design: Prospective, randomized clinical trial.
Methods: Twenty-eight eyes of 25 patients with corneal edema resulting from Fuchs dystrophy underwent DLEK with a 9- to 10-mm incision (n = 13) or PK with double-running sutures (n = 15) at the Cornea Service, Mayo Clinic Department of Ophthalmology, Rochester, Minnesota.
Corneal endothelial cell proliferation: a function of cell density.
Invest Ophthalmol Vis Sci
June 2009
Purpose: To determine how stimuli that increase corneal endothelial cell proliferation of human corneas in culture relate to changes in endothelial cell density in the central and peripheral cornea.
Methods: Human donor cadaver corneas not suitable for transplantation were divided into four pie-shaped wedges and incubated at 37 degrees C in medium supplemented with fetal bovine serum, epidermal growth factor, fibroblast growth factor, and gentamicin. To promote a proliferative response, samples were treated with EDTA at concentrations of 0, 0.
Purpose: To compare subbasal nerve densities estimated from images recorded by the tandem scanning and the ConfoScan 4 confocal microscopes.
Methods: Confocal microscopy was used to estimate subbasal nerve density in 62 corneas of 40 subjects (18 corneas of 18 normal subjects and 44 corneas of 22 patients between 1 and 12 months after LASIK). At each examination, corneas were scanned first by using tandem scanning and then by using a ConfoScan 4 confocal microscope.
Purpose: To compare long-term keratometric changes after penetrating keratoplasty (PK) for keratoconus and Fuchs endothelial dystrophy.
Design: Retrospective, comparative case series.
Methods: We retrospectively analyzed 168 corneas after PK for keratoconus (85 eyes of 63 subjects) and Fuchs dystrophy (83 eyes of 60 subjects).
Purpose: To determine the effect of corneal light scatter on vision after penetrating keratoplasty (PK).
Design: Cross-sectional study.
Methods: setting: Cornea service at Mayo Clinic, Rochester, Minnesota.
Keratocyte and subbasal nerve density after penetrating keratoplasty.
Trans Am Ophthalmol Soc
August 2008
Purpose: To determine central keratocyte and subbasal nerve densities after penetrating keratoplasty (PK) in clear grafts and in grafts with late endothelial failure (LEF).
Methods: Ninety-nine clear grafts of 74 patients and 21 grafts with LEF in 19 patients were examined by confocal microscopy at 1 to 31 years after PK. Keratocyte density and number of keratocytes in a full-thickness column of stroma with frontal area of 1 mm(2) were determined from images.
Purpose: To compare long-term keratometric changes after penetrating keratoplasty (PK) for keratoconus and Fuchs endothelial dystrophy.
Methods: We retrospectively analyzed 168 corneas after PK for keratoconus (85 eyes of 63 subjects) and Fuchs dystrophy (83 eyes of 60 subjects). Patients were examined after final suture removal at 12 months after PK to 30 years after surgery.
Objective: To determine central keratocyte and subbasal nerve densities in clear and failed grafts after penetrating keratoplasty.
Methods: Clear grafts and grafts with late endothelial failure (LEF) were examined using confocal microscopy 1 to 31 years after penetrating keratoplasty. Keratocyte density, number of keratocytes in a full-thickness column of stroma, and subbasal nerve density were determined from images.
Purpose: To assess the precision and accuracy of the z-ring adapter with the ConfoScan 4 confocal microscope for measuring corneal thickness.
Methods: Thirty healthy corneas of 15 volunteers were scanned twice with a ConfoScan 4 confocal microscope equipped with a z-ring adapter (Nidek, Inc., Fremont, CA) and with a Tandem Scanning confocal microscope (Tandem Scanning Corporation, Reston, VA).
Purpose: To determine the long-term changes in epithelial, stromal, and corneal thickness after LASIK and photorefractive keratectomy (PRK).
Methods: In two prospective observational case series, 11 patients (16 eyes) received LASIK and 12 patients (18 eyes) received PRK to correct myopia or myopic astigmatism. None of the corneas had retreatment procedures.
Purpose: To describe stromal changes after a femtosecond laser laser-assisted in situ keratomileusis (LASIK) flap was created but not separated.
Design: Case report.
Methods: As part of a randomized paired-eye study comparing LASIK flap creation by a femtosecond laser to a mechanical microkeratome, a femtosecond laser flap was successfully created on one eye of one patient, but the flap was not separated because of a flap-related complication in the fellow eye.