The Listeria monocytogenes PASTA Kinase PrkA and Its Substrate YvcK Are Required for Cell Wall Homeostasis, Metabolism, and Virulence.

Obstacles to bacterial survival and replication in the cytosol of host cells, and the mechanisms used by bacterial pathogens to adapt to this niche are not well understood. Listeria monocytogenes is a well-studied Gram-positive foodborne pathogen that has evolved to invade and replicate within the host cell cytosol; yet the mechanisms by which it senses and responds to stress to survive in the cytosol are largely unknown. To assess the role of the L.

Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration.

Cell motility is required for diverse biological processes including development, homing of immune cells, wound healing, and cancer cell invasion. Motile neutrophils exhibit a polarized morphology characterized by the formation of leading-edge pseudopods and a highly contractile cell rear known as the uropod. Although it is known that perturbing uropod formation impairs neutrophil migration, the role of the uropod in cell polarization and motility remains incompletely understood.

Macrophages mediate flagellin induced inflammasome activation and host defense in zebrafish.

The inflammasome is an innate immune complex whose rapid inflammatory outputs play a critical role in controlling infection; however, the host cells that mediate inflammasome responses in vivo are not well defined. Using zebrafish larvae, we examined the cellular immune responses to inflammasome activation during infection. We compared the host responses with two Listeria monocytogenes strains: wild type and Lm-pyro, a strain engineered to activate the inflammasome via ectopic expression of flagellin.

ABCB5 is a limbal stem cell gene required for corneal development and repair.

Corneal epithelial homeostasis and regeneration are sustained by limbal stem cells (LSCs), and LSC deficiency is a major cause of blindness worldwide. Transplantation is often the only therapeutic option available to patients with LSC deficiency. However, while transplant success depends foremost on LSC frequency within grafts, a gene allowing for prospective LSC enrichment has not been identified so far.

Selective pharmacologic inhibition of a PASTA kinase increases Listeria monocytogenes susceptibility to β-lactam antibiotics.

While β-lactam antibiotics are a critical part of the antimicrobial arsenal, they are frequently compromised by various resistance mechanisms, including changes in penicillin binding proteins of the bacterial cell wall. Genetic deletion of the penicillin binding protein and serine/threonine kinase-associated protein (PASTA) kinase in methicillin-resistant Staphylococcus aureus (MRSA) has been shown to restore β-lactam susceptibility. However, the mechanism remains unclear, and whether pharmacologic inhibition would have the same effect is unknown.

Division I men and women athletes do not differ on perceptions of worth.

Historically, especially prior to the increased interest in women's athletics with the passage of Title IX in 1972, there have been negative perceptions of women as athletes. If these social perceptions still hold in part today, as is indirectly suggested by unequal press coverage and less basic support for women athletes, one might predict that collegiate female athletes would rate themselves lower on self-esteem and worth than collegiate male athletes. 176 Division I male (n = 90) and female (n = 86) athletes rated their perceptions of self on the Worth Index which measures basic human worth, personal security, performance, and physical self; these are divided into intrinsic (unconditional worth) measures and behavior or performance (conditional worth) measures.

Evaluation of a university general education health and wellness course delivered by lecture or online.

Purpose: To assess a single-semester university general education (GE) health and wellness course influence on physical activity (PA) and dietary habits among university students and to compare the course delivered through lecture or online for these outcomes.

Design: A 15-week intervention with pre-post one-group design, allowing for comparative assessments in dietary and PA habits across time by delivery method (classroom lecture vs. online).

Perceptions of self of Division I team and individual sport athletes.

The Worth Index was administered to 176 Division I athletes who were competing in team and individual sports at Brigham Young University. The purpose of the study was to measure and compare their perceptions of worth and self-esteem. The Worth Index is a valid tool measuring whether an individual believes worth and self-esteem are earned by way of performance (conditional) or are inherent (unconditional).

Effects of placement, attachment, and weight classification on pedometer accuracy.

Background: The purpose was to determine if waist placement of the pedometer affected accuracy in normal, overweight, and obese children, when attaching the pedometer to the waistband or a belt.

Methods: Seventy-seven children (ages 10-12 years) wore 5 pedometers on the waistband of their pants and a belt at the following placements: navel (NV), anterior midline of the right thigh (AMT), right side (RS), posterior midline of the right thigh (PMT), and middle of the back (MB). Participants walked 100 steps on a treadmill at 80 m x min(-1).