Publications by authors named "William J Peria"

Populations of isogenic cells often respond coherently to signals, despite differences in protein abundance and cell state. Previously, we uncovered processes in the pheromone response system (PRS) that reduced cell-to-cell variability in signal strength and cellular response. Here, we screened 1,141 non-essential genes to identify 50 "variability genes".

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Many cell signaling systems, including the yeast pheromone response system, exhibit "dose-response alignment" (DoRA), in which output of one or more downstream steps closely matches the fraction of occupied receptors. DoRA can improve the fidelity of transmitted dose information. Here, we searched systematically for biochemical network topologies that produced DoRA.

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Study of signal transduction in live cells benefits from the ability to visualize and quantify light emitted by fluorescent proteins (XFPs) fused to different signaling proteins. However, because cell signaling proteins are often present in small numbers, and because the XFPs themselves are poor fluorophores, the amount of emitted light, and the observable signal in these studies, is often small. An XFP's fluorescence lifetime contains additional information about the immediate environment of the fluorophore that can augment the information from its weak light signal.

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Participants studied naturalistic pictures presented for varying brief durations and then received a recognition test on which they indicated whether each picture was old or new and rated their confidence. In 1 experiment they indicated whether each "old"/"new" response was based on memory for a specific feature in the picture or instead on the picture's general familiarity; in another experiment, we defined pictures that tended to elicit feature versus familiarity responses. Thus, feature/familiarity was a dependent variable in 1 experiment and an independent variable in the other.

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