The spinach YY genome reveals sex chromosome evolution, domestication, and introgression history of the species.

Background: Spinach (Spinacia oleracea L.) is a dioecious species with an XY sex chromosome system, but its Y chromosome has not been fully characterized. Our knowledge about the history of its domestication and improvement remains limited.

The Red Fox Y-Chromosome in Comparative Context.

While the number of mammalian genome assemblies has proliferated, Y-chromosome assemblies have lagged behind. This discrepancy is caused by biological features of the Y-chromosome, such as its high repeat content, that present challenges to assembly with short-read, next-generation sequencing technologies. Partial Y-chromosome assemblies have been developed for the cat (), dog (), and grey wolf (), providing the opportunity to examine the red fox () Y-chromosome in the context of closely related species.

Development of an X-specific marker and identification of YY individuals in spinach.

Spinach is a popular vegetable native to central and western Asia. It is dioecious with a pair of nascent sex chromosomes. The difficulties of working with the non-recombining sex determination region of XY individuals have hindered the progress toward sequencing sex chromosomes of most dioecious species.