Affinity purification of natural ligands.

Immobilization of proteins, nucleic acids, and other bioligands is not always straightforward since they are often large molecules with numerous chemically reactive groups that can all participate in the immobilization process through physical adsorption, ionic binding, or covalent linkage. Protocols for some of the most frequently used matrix-activation systems are described in this unit. For agarose, protocols are given for cyanogen bromide, p-nitrophenyl chloroformate, tresyl chloride, and cyanuric chloride.

Can tobacco have a potentially beneficial effect to our health?

With urgent pressure to clean up the contaminated environment, new approaches are needed. Phyto- and rhizoremediation using plants and related bacteria is a promising approach, but has its inborn limitations. To overcome the slow performance of the process, transgenic plants have been prepared specifically tailored for phytoremediation purposes.