Long-term gut colonization with ESBL-producing in participants without known risk factors from the southeastern United States.

We evaluated gut carriage of extended spectrum beta lactamase producing (ESBL-E) in southeastern U.S. residents without recent in-patient healthcare exposure.

growth kinetics and the metabolic effects of iron.

Transmission of occurs opportunistically through direct seawater exposure and is a function of its abundance in the environment. Like other spp., are considered conditionally rare taxa in marine waters, with populations capable of forming large, short-lived blooms under specific environmental conditions, which remain poorly defined.

Direct wastewater extraction as a simple and effective method for SARS-CoV-2 surveillance and COVID-19 community-level monitoring.

Wastewater surveillance has proven to be an effective tool to monitor the transmission and emergence of infectious agents at a community scale. Workflows for wastewater surveillance generally rely on concentration steps to increase the probability of detection of low-abundance targets, but preconcentration can substantially increase the time and cost of analyses while also introducing additional loss of target during processing. To address some of these issues, we conducted a longitudinal study implementing a simplified workflow for SARS-CoV-2 detection from wastewater, using a direct column-based extraction approach.

Coral Disease and Ingestion: Investigating the Role of Heterotrophy in the Transmission of Pathogenic spp. using a Sea Anemone () Model System.

Understanding disease transmission in corals can be complicated given the intricacy of the holobiont and difficulties associated with coral cultivation. As a result, most of the established transmission pathways for coral disease are associated with perturbance (i.e.

Use and Evaluation of a pES213-Derived Plasmid for the Constitutive Expression of Protein in Pathogenic Vibrios: a Tagging Tool for Studies.

Insertion of green fluorescent protein (GFP) into bacterial cells for constitutive expression is a powerful tool for the localization of species of interest within complex mixtures. Here, we demonstrate and evaluate the efficacy of the pES213-derived donor plasmid pVSV102 ( Kn) as a conjugative tool for the tagging of and related species (termed vibrios). Using a triparental mating assay assisted by the helper plasmid pEVS104 ( Kn), we successfully tagged 12 species within the family representing 8 of the proposed clades.