Publications by authors named "William A Cuevas"
Repeat sequence protein polymer (RSPP) technology provides a platform to design and make protein-based performance polymers and represents the best nature has to offer. We report here that the RSPP platform is a novel approach to produce functional protein polymers that have both biomechanical and biofunctional blocks built into one molecule by design, using peptide motifs. We have shown that protein-based designer biopolymers can be made using recombinant DNA technology and fermentation and offer the ability to screen for desired properties utilizing the tremendous potential diversity of amino acid combinations.
View Article and Find Full Text PDFIn Escherichia coli, the ferric uptake regulator (Fur) controls expression of the iron regulon in response to iron availability while the cyclic AMP receptor protein (Crp) regulates expression of the carbon regulon in response to carbon availability. We here identify genes subject to significant changes in expression level in response to the loss of both Fur and Crp. Many iron transport genes and several carbon metabolic genes are subject to dual control, being repressed by the loss of Crp and activated by the loss of Fur.
View Article and Find Full Text PDFArticle Synopsis
- The study analyzes the gene expression regulated by the cyclic AMP receptor protein (Crp), focusing on genes related to central carbon metabolism and how they are influenced by catabolite repression or activation.
- Key findings indicate that many operons for central carbon metabolic enzymes face direct repression by Crp, while few enzyme genes and an abundance of ribosomal/tRNA genes are activated indirectly by glucose through Crp.
- The research reinforces the role of Crp in regulating stress response proteins through strong indirect catabolite repression, confirmed through various experimental approaches like real-time PCR and reporter gene assays.