Differential effect of acute persistent insect-specific flavivirus infection on superinfection exclusion of West Nile, Zika and chikungunya viruses in RNAi-competent and -deficient mosquito cells.

Millions of people are annually infected by mosquito-transmitted arboviruses including dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV) and chikungunya virus (CHIKV). Insect-specific flaviviruses (ISFs), which only infect mosquitoes and cannot replicate in vertebrates, can offers a potential one health strategy to block the transmission of arboviruses by reducing the mosquito's susceptibility for subsequent arbovirus infections through superinfection exclusion (SIE),. Most SIE studies focus on acute ISF infections in RNAi-deficient C6/36 cells.

Sexual function after surgically corrected menstrual outflow obstruction due to congenital anomalies.

Objectives: To enhance evidence-based knowledge about sexual function and the prevalence of sexual dysfunction after surgical therapy for congenital anomalies with menstrual outflow obstruction.

Materials And Methods: In this long-term case-control study, all patients underwent surgical correction of an obstructive Müllerian anomaly between 1980 and 2013. At the start of the case-control study, patients were at least 18 years old and were two years post their initial operation.

Long-term effects of surgically corrected menstrual outflow obstruction: A case-control study.

Objective: To enhance evidence-based knowledge on long-term sequalae in patients with surgically corrected obstructing Müllerian anomalies.

Methods: This long-term case-control study included patients with menstrual outflow obstruction due to congenital anomalies of the uterus or vagina, who were at least 18 years old, and for whom 2 years had elapsed since the first surgery at the start of this study. The control group consisted of women without current gynecological problems.

Added value of involving patients in the first step of multidisciplinary guideline development: a qualitative interview study among infertile patients.

Background: Patient involvement in scoping the guideline is emphasized, but published initiatives actively involving patients are generally limited to the writing and reviewing phase.

Objective: To assess patients' added value to the scoping phase of a multidisciplinary guideline on infertility.

Design: Qualitative interview study.

Long-term results of vaginal construction with the use of Frank dilation and a peritoneal graft (Davydov procedure) in patients with Mayer-Rokitansky-Küster syndrome.

Objective: To describe long-term outcomes after nonsurgical and surgical treatment of vaginal agenesis in a cohort of girls and women with Mayer-Rokitansky-Küster (MRK) syndrome.

Design: Retrospective cohort study using a standardized case record form.

Setting: University hospital.

Salpingotomy versus salpingectomy in women with tubal pregnancy (ESEP study): an open-label, multicentre, randomised controlled trial.

Background: Tubal ectopic pregnancy can be surgically treated by salpingectomy, in which the affected Fallopian tube is removed, or salpingotomy, in which the tube is preserved. Despite potentially increased risks of persistent trophoblast and repeat ectopic pregnancy, salpingotomy is often preferred over salpingectomy because the preservation of both tubes is assumed to offer favourable fertility prospects, although little evidence exists to support this assumption. We aimed to assess whether salpingotomy would improve rates of ongoing pregnancy by natural conception compared with salpingectomy.

Risk of borderline and invasive ovarian tumours after ovarian stimulation for in vitro fertilization in a large Dutch cohort.

Background: Long-term effects of ovarian stimulation for IVF on the risk of ovarian malignancies are unknown.

Methods: We identified a nationwide historic cohort of 19,146 women who received IVF treatment in the Netherlands between 1983 and 1995, and a comparison group of 6006 subfertile women not treated with IVF. In 1997-1999, data on reproductive risk factors were obtained from 65% of women and data on subfertility (treatment) were obtained from the medical records.

Galactose-1-phosphate uridyl transferase deficiency is not associated with Müllerian aplasia in Dutch patients.

Study Objective: To study whether a deficiency in galactose-1-phosphate uridyl transferase (GALT) activity of mothers was an explanation for the occurrence of Müllerian aplasia of their daughters.

Design: A case control study.

Setting: The patients were selected from the outpatient clinic of the University Medical Center Nijmegen, and compared with the general population in The Netherlands.

Treatment of patients with a congenital transversal vaginal septum or a partial aplasia of the vagina. The vaginal pull-through versus the push-through technique.

Study Objective: The aim of this study is to describe the different modalities of congenital obstructing vaginal malformations and the evaluation of techniques to solve the problem.

Design: A retrospective study.

Setting: The University Hospital Nijmegen, the Netherlands.

Review of the role of the plasminogen activator system and vascular endothelial growth factor in subfertility.

Objective: To assess the importance of the plasminogen activator (PA) system and vascular endothelial growth factor (VEGF) in subfertility.

Design: Review.

Setting: Two university IVF centers.

Influence of seminar and mailed guidelines on knowledge of school teachers regarding emergency treatment for dental injuries.

Objective: To evaluate the influence of two different modes for delivering guidelines on school teachers' knowledge on emergency treatment for dental injuries.

Design: Cross sectional questionnaire study.

Setting: Primary schools in Dar es Salaam city council in Tanzania.

The long term outcome of feminizing genital surgery for congenital adrenal hyperplasia: anatomical, functional and cosmetic outcomes, psychosexual development, and satisfaction in adult female patients.

Background: There are only a few reports analyzing the long term outcome of feminizing surgery in females with congenital adrenal hyperplasia (CAH). Such analysis is crucial to evaluate the treatment and to make necessary adjustments.

Study Objectives: To evaluate the adult outcome after feminizing surgery in adult females with salt wasting CAH.

Birth weight and age at menarche in patients with polycystic ovary syndrome or diminished ovarian reserve, in a retrospective cohort.

Background: Few studies have investigated the association between subfertility in women and factors in early life such as birth weight and age at menarche, and most have produced contradictory results. In the present study, this association was investigated among women undergoing artificial reproductive techniques (ART), including IVF for reason of polycystic ovary syndrome (PCOS) or diminished ovarian reserve. Herein, PCOS included oligomenorrhoea and at least one additional symptom such as hyperandrogenism, hirsutism or polycystic ovaries on ultrasound.

Monitoring pulp vitality after transplantation of teeth with mature roots: a case report.

Aim: To initiate discussion on the value of routine root canal treatment for transplanted teeth.

Summary: Autotransplantation is an accepted treatment option to replace missing teeth. It is generally considered that revascularization of the pulp following such a procedure is more favourable in teeth with immature roots.

[Formaldehyde in dental materials].

The use of formaldehyde in dentistry has been discussed for years. This because of the possible systemic effects of its use. This paper addresses the possible systemic effects of the use of formaldehyde and the question in which dental products formaldehyde is a component.

A syndromal and an isolated form of uterine arteriovenous malformations: two case-reports.

Uterine arteriovenous malformations are rare lesions with a considerable risk potential. Clinical presentation varies from no signs over various degrees of menorrhagia to massive life threatening vaginal bleeding. This is the first report of congenital uterine arteriovenous malformations in two patients with primary infertility.

[Intracanal medication used in general dentistry].

In order to investigate the use of intracanal substances during root canal treatment 400 dentists were asked to take part in a questionnaire survey. Questions related to the substances used, the reason for use and the way they were used. Studied were the possible influences by the variables 'year of graduation', 'number of patients in practice', 'dental school'.

The effect of a single educational input given to school teachers on patient's correct handling after dental trauma.

The aim of this study was to evaluate the influence of mailed guidelines and seminars to school teachers on self-care actions taken by children after trauma. Data was collected 6 months before and 5 months after provision of the guidelines. Particulars of school children who sought dental consultation, types of injuries, the medium used to store avulsed teeth and the time elapsed from injury to dental consultation were recorded.

[Repair and revision 4. Cracked tooth and crown fractures: diagnostics and treatment].

Trauma to the teeth can result in the formation of cracks in the crown or root of the tooth. The location and the extent of these cracks determine the proper treatment choice. This treatment is oriented towards saving pulp vitality and/or tooth integrity.

[Success of direct pulp capping after caries excavations].

Direct pulp capping is not generally accepted as a routine dental procedure. It is claimed to be an unpredictable procedure with a low success rate. However, some clinical studies do show success of direct pulp cappings.

[Cervical external root resorption].

Cervical external root resorption is a progressive resorption process occurring at the site of the attached gingiva. It is independent on the condition of the pulp. Prerequisites for its occurrence are a damaged root surface and the presence of vascularized connective tissue.

[Fibroids and fertility].

Fibroids are the most common benign uterine tumours. A relation between fibroids and infertility is suggested. Successful implantation depends on a normal, receptive endometrium.