New records and revised distribution of tiger beetles in China (Coleoptera, Cicindelidae).

Based on the examination of specimens housed in several scientific collections, we expand the known tiger beetle fauna of China, and eight species are recorded from China for the first time. The occurrence of Cicindela (Cicindela) sachalinensis raddei Morawitz, 1863 in Shanxi Province, and Neocollyris (Neocollyris) saphyrina (Chaudoir, 1850) in China are re-established. We provide distribution maps and habitus photographs of examined specimens for the newly recorded and revised species.

Intensive Longitudinal Social Sensing in Patients With Psychosis Spectrum Disorders: An Exploratory Pilot Study.

Background: Psychosis spectrum disorders are characterized by significant alterations in social functioning, which is a major factor for patient recovery. Despite its importance, objectively quantifying the complex day-to-day social behavior in real-life settings has rarely been attempted. Here, we conducted a pilot study with wearable sensors that passively and continuously register interactions with other participants.

Revision of the genus Apterodela Rivalier, 1950 (Coleoptera, Cicindelidae).

A revision of the genus Apterodela Rivalier, 1950 is presented. The new subgenus Protoapterodela subgen. nov.

Tiger beetles of the genus Tricondyla Latreille, 1822 (Coleoptera: Cicindelidae) of the Philippines, with some considerations of the taxonomy of the genus.

The fully everted endophallus in the genus Tricondyla is studied and described for the first time. Also, the following new synonymies are established: Pseudotricondyla Naviaux, 2002 syn. nov.

Philippine species of the genus Prothyma Hope, 1838 (Coleoptera: Cicindelidae). Part 2. Subgenus Symplecthyma Rivalier, 1964.

The Philippine species of the subgenus Symplecthyma Rivalier, 1964 within the genus Prothyma Hope, 1838 are revised to include eight species. Prothyma (Symplecthyma) bella Dheurle & Colas, 2018 syn. nov.

MicroCT data provide evidence correcting the previous misidentification of an Eocene amber beetle (Coleoptera, Cicindelidae) as an extant species.

The fossil record suggests some insect species have a marked longevity. The oldest fossils purported to represent extant insect species are from the Oligocene and Eocene. One of the most cited fossils is the extant tiger beetle Tetracha carolina (Coleoptera: Cicindelidae) that was identified over a century ago by Walther Horn in Eocene Baltic amber.

A gender biased hidden curriculum of clinical vignettes in undergraduate medical training.

Objectives: Medical education relies extensively on clinical vignettes, yet little attention has been given to what hidden curriculum they might convey. Our research aimed to identify whether the clinical vignettes used in pre-graduate medical education transmit gender stereotypes or gender biases.

Methods: We conducted a mixed quantitative and qualitative analysis of gender-related characteristics currently existing in clinical vignettes used for pre-graduate teaching and evaluation at the Geneva Faculty of Medicine.

Calochroa miroklichai sp. nov., a new species from Thailand with rectification of different concepts of Calochroa tritoma (Schmidt-Goebel) and comparisons to similar species. (Coleoptera: Cicindelidae).

A new species of the genus Calochroa Hope, 1838 is described as new to science from Thailand. Two different concepts of Calochroa tritoma (Schmidt-Goebel, 1846) are rectified here, stating that the correct concept of C. tritoma (fully corresponding to the holotype in NMPC) is that presented by Naviaux (1991), Naviaux & Pinratana (2004) and Klícha & Ďuríček (2021), while C.

The Philippines species of the genus Prothyma Hope, 1838 (Coleoptera: Cicindelidae) Part 1: Subgenera Prothyma Hope, 1838, Genoprothyma Rivalier, 1964 and Parasymplecthyma subgen. nov.

The Philippines species of Prothyma Hope, 1838 (Prothyma s.str., Genoprothyma Rivalier, 1964 and Parasymplecthyma subgen.

Engineered nickel bioaccumulation in by NikABCDE transporter and metallothionein overexpression.

Mine wastewater often contains dissolved metals at concentrations too low to be economically extracted by existing technologies, yet too high for environmental discharge. The most common treatment is chemical precipitation of the dissolved metals using limestone and subsequent disposal of the sludge in tailing impoundments. While it is a cost-effective solution to meet regulatory standards, it represents a lost opportunity.

Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering.

The cultivation of cells in 3D systems is commonly regarded to be more physiological than in 2D as it comes much closer to the natural situation in tissues in many different aspects. However, 3D cell culture is much more complex. Cells within the pores of a printed 3D scaffold face a special situation concerning cell-material interaction and cell adhesion, cell proliferation, and supply of medium and oxygen into the core of the scaffolds.

New or rare Madagascar tiger beetles26. A new species of the genus Pogonostoma Klug from northwestern Madagascar and a revised key to the Pogonostoma (P.) srnkai species-group (Coleoptera: Cicindelidae).

A new species of the genus Pogonostoma Klug, 1835, P. (Pogonostoma) mahimborondrense Moravec Wiesner sp. nov.

Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment.

Stimulatory type 1 conventional dendritic cells (cDC1s) engage in productive interactions with CD8 effectors along tumor-stroma boundaries. The paradoxical accumulation of "poised" cDC1s within stromal sheets is unlikely to simply reflect passive exclusion from tumor cores. Drawing parallels with embryonic morphogenesis, we hypothesized that invasive margin stromal remodeling generates developmentally conserved cell fate cues that regulate cDC1 behavior.

Novel Insights into Pyrrolizidine Alkaloid Toxicity and Implications for Risk Assessment: Occurrence, Genotoxicity, Toxicokinetics, Risk Assessment-A Workshop Report.

This paper reports on the major contributions and results of the 2nd International Workshop of Pyrrolizidine Alkaloids held in September 2020 in Kaiserslautern, Germany. Pyrrolizidine alkaloids are among the most relevant plant toxins contaminating food, feed, and medicinal products of plant origin. Hundreds of PA congeners with widespread occurrence are known, and thousands of plants are assumed to contain PAs.

Regulatory Perspectives of Pyrrolizidine Alkaloid Contamination in Herbal Medicinal Products.

The toxicity of plants containing certain pyrrolizidine alkaloids has long been recognized in grazing animals and humans. Genotoxicity and carcinogenicity data from and (animal) studies were published over the last few decades for some of the 1,2-unsaturated pyrrolizidine alkaloids, leading to regulatory action on herbal medicinal products with pyrrolizidine alkaloid-containing plants more than 30 years ago. In recent years, it has become evident that in addition to herbal medicinal products containing pyrrolizidine alkaloid-containing plants, these products may also contain pyrrolizidine alkaloids without actually including pyrrolizidine alkaloid-containing plants.

Tiger beetles (Coleoptera, Cicindelidae) of Northern Mindanao region (Philippines): checklist, distributional maps, and habitats.

The knowledge about tiger beetle fauna of the Northern Mindanao region (Philippines) is summarized based on literature data and new records. Thirty species classified in ten genera (, , , , , , , , , and ) were documented from the area (56% of tiger beetle fauna of Mindanao and 21% of Philippine species). Twelve species were noted from Northern Mindanao region for the first time, including five taxa, , , , , and , not recorded from Mindanao before.

Alien and native tree species having extrafloral nectaries as favorite hunting area for arboreal endemic Philippine tiger beetles (Coleoptera: Cicindelidae) in human-disturbed habitat in Lanao del Sur Province, Mindanao, Philippines.

To document a relation between abundance of arboreal, predatory tiger beetles, their ant prey, and extrafloral nectaries attracting the ants, we gathered data from more than 10 species of native and introduced trees and large, tree-like perennial plants in Lanao del Sur Province, Mindanao, Philippines. All specimens of tiger beetles (two and two species, all endemic to the country) were noted on five tree species characterized by presence of extrafloral nectaries, including three alien/invasive and two native ones. Invasive and native were the most inhabited ones (respectively, 56% and 19% of beetles).

Automated mass spectrometry multi-attribute method analyses for process development and characterization of mAbs.

More than 370 biotherapeutics drug products have been approved by regulatory agencies on the US and EU markets and this industry continues to expand. Process change and optimization is necessary to develop new effective biologics in a cost effective and productive way. Consequently, improvement of analytical techniques is required for better product characterization according to Quality by Design (QbD) approach recommended by regulatory agencies.

Diagnostic Characteristics of 3-Parameter and 2-Parameter Equations for the Calculation of a Combined Indicator of Vitamin B12 Status to Predict Cobalamin Deficiency in a Large Mixed Patient Population.

Background: A combined indicator for the determination of vitamin B12 status (4cB12) that employs four markers of vitamin B12 status (i.e., holotranscobalamin, HoloTC; vitamin B12, B12; methyl malonic acid, MMA; and homocysteine, Hcy) has been proposed for the comprehensive assessment of B12 status.

Versican and Versican-matrikines in Cancer Progression, Inflammation, and Immunity.

Versican is an extracellular matrix proteoglycan with key roles in multiple facets of cancer development, ranging from proliferative signaling, evasion of growth-suppressor pathways, regulation of cell death, promotion of neoangiogenesis, and tissue invasion and metastasis. Multiple lines of evidence implicate versican and its bioactive proteolytic fragments (matrikines) in the regulation of cancer inflammation and antitumor immune responses. The understanding of the dynamics of versican deposition/accumulation and its proteolytic turnover holds potential for the development of novel immune biomarkers as well as approaches to reset the immune thermostat of tumors, thus promoting efficacy of modern immunotherapies.

Antimicrobial Peptides from Rat-Tailed Maggots of the Drone Fly Show Potent Activity against Multidrug-Resistant Gram-Negative Bacteria.

The spread of multidrug-resistant Gram-negative bacteria is an increasing threat to human health, because novel compound classes for the development of antibiotics have not been discovered for decades. Antimicrobial peptides (AMPs) may provide a much-needed breakthrough because these immunity-related defense molecules protect many eukaryotes against Gram-negative pathogens. Recent concepts in evolutionary immunology predict the presence of potent AMPs in insects that have adapted to survive in habitats with extreme microbial contamination.

Diagnostic Accuracy of Holotranscobalamin, Vitamin B12, Methylmalonic Acid, and Homocysteine in Detecting B12 Deficiency in a Large, Mixed Patient Population.

Four biomarkers are commonly employed to diagnose B12 deficiency: vitamin B12 (B12), holotranscobalamin (HoloTC), methylmalonic acid (MMA), and homocysteine (Hcy). 4cB12, a combined index of the B12 status, has been suggested to improve the recognition of B12 deficiency. We aimed to evaluate the four different markers for detecting B12 deficiency, as determined by 4cB12.