Light Emission from Fe-EGTA-HO System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body.

A Fe-EGTA(ethylene glycol-bis (β-aminoethyl ether)-,,','-tetraacetic acid)-HO system emits photons, and quenching this chemiluminescence can be used for determination of anti-hydroxyl radical (•OH) activity of various compounds. The generation of •OH and light emission due to oxidative damage to EGTA may depend on the buffer and pH of the reaction milieu. In this study, we evaluated the effect of pH from 6.

Concentration Dependence of Anti- and Pro-Oxidant Activity of Polyphenols as Evaluated with a Light-Emitting Fe-Egta-HO System.

Hydroxyl radical (OH) scavenging and the regeneration of Fe may inhibit or enhance peroxidative damage induced by a Fenton system, respectively. Plant polyphenols reveal the afore-mentioned activities, and their cumulative net effect may determine anti- or pro-oxidant actions. We investigated the influence of 17 phenolics on ultra-weak photon emission (UPE) from a modified Fenton system (92.

Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe-EGTA-HO and Fe-EGTA-HO Systems In Vitro.

Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe/Fe ions and HO, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 µmol/L on OH-induced light emission (at a pH of 7.

Referral to radioisotope examination as a source of additional radiation exposure for staff.

Background: Every exposure of human to ionizing radiation increases the likelihood of deterministic sequelae. At the same time, it is associated with the risk of stochastic effects. Consequently, this can lead to cancer, mainly of the hematopoietic system.

Light emission from the Fe -EDTA-ascorbic acid-H O system strongly enhanced by plant phenolic acids.

Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe -EDTA (ethylenediaminetetraacetic acid)-AA (ascorbic acid)-H O (hydrogen peroxide) system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380-630 nm). The UPE, of 92.

Light Emission from the Fe-EGTA-H₂O₂ System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics.

Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe-EGTA (ethylene glycol-bis(β-aminoethyl ether)-,,','-tetraacetic acid)-H₂O₂ system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380 to 630 nm). The UPE of 92.

The assessment of testosterone and radioisotopic index of bone metabolism and bone mineral density in men with testosterone deficiency after one year of testosterone therapy.

Background: Testosterone deficiency in men is characterized by typical symptoms of hypogonadism and negative influence on the preservation of bone mass. In this study, we analysed the relationship between testosterone concentration and bone metabolism. Moreover, we assessed the impact of one-year compensation of testosterone deficiency in elderly men on bone metabolism and bone mineral density.

Radioisotopic assessment of bone metabolism of the operated vertebra after inter-process stabilizer implantation in the lumbar segment of the spine.

Introduction: Lack of efficacy of pharmacotherapy and physiotherapy in spinal pain syndrome is an indication for intervertebral stabilizer implantation between the processes in the lumbar segment of the spine.

Material And Methods: The group consisted of 32 patients qualified after radioisotopic single-photon emission computed tomography/computed tomography (SPECT/CT) examinations with assessment of bone metabolism and mineral density. For comparative purposes, the L2 vertebra was defined as normal.

The own method and program of isotope quantitatively assessment of perfusion in muscles of upper limbs (initial report).

Background: The issue of blood flow in muscles has been dealt with for many years. However, most often it was assessed qualitatively with standard vascular examinations. The quantitatively perfusion assessment is indispensable in the normal and pathological conditions.

Reliability of different radioisotopic techniques in diagnosing solitary hot spots in the spine of patients with malignant neoplasm.

Introduction: Early diagnosis of spinal metastases is of key importance in further therapeutic management.

Objectives: The aim of the study was to assess the reliability of single-photon emission computed tomography (SPECT) or SPECT and computed tomography (SPECT/CT) in the diagnosis of solitary hot nodules in the spine identified by scintigraphy.

Patients And Methods: In this retrospective study, 70 patients with neoplastic disease and a solitary nodule detected in the spine were analyzed.

Bone metabolism assessment, bone metabolism index designation and the determination of its normal values range in young healthy women.

Background: Bone metabolism assessment requires the determination of bone mass and quality. The bone metabolism was assessed with the modified bone scintigraphy using 99mTc-MDP. The elaboration of radioisotopic method and program allowed for the assessment of bone metabolism, index of bone metabolism assay and definition of its normal values range with the possibility of clinical application.

Correlation of the severity of diabetic retinopathy and the heart muscle perfusion in patients with type 2 diabetes.

Objective: The objective was to investigate whether microvascular disturbances in patients with type 2 diabetes (DM) as defined by retinal examination predict the existence of macrovascular disturbances found on radioisotopic perfusion examinations of the heart muscle.

Materials And Methods: A total of 100 patients with type 2 DM and an additional cardiovascular risk factor were enrolled in the study. All patients underwent comprehensive ophthalmologic examination, including fundus color photography and fluorescein angiography, and were divided into three groups: group 1 (NoDR): met the inclusion criteria but had no diabetic retinopathy; group 2 (NPDR): had signs of nonproliferative diabetic retinopathy; group 3 (PDR): had signs of preproliferative or proliferative diabetic retinopathy.

Influence of pharmacological treatment on blood flow and muscle perfusion in patients with obliterative atheromatosis assessed with isotopic methods. A pilot study.

Background: Most of the methods don't give the comparison between blood flow at rest and after exercise. Nuclear medicine allows for such assessment and determination of muscle perfusion indexes. The aim of the study was the comparison of the radioisotopic assessment of lower limbs perfusion at rest and after exercise in patients with obliterative atheromatosis subjected to combined treatment with the standard vascular examinations.

Progression of diabetic retinopathy correlated with muscle perfusion disturbances of the lower limbs, with clinically important diagnostic recommendations.

Introduction: The objective of this study was to investigate the relationship between microvascular and macrovascular disturbances in patients with type 2 diabetes, as shown by results of ophthalmological examination and by vascular and perfusion examinations of the lower limbs.

Material And Methods: A total of 85 patients with type 2 diabetes and an additional cardiovascular risk factor were enrolled in the study. All patients underwent complex ophthalmological examination, including fundus colour photography and fluorescein angiography, and were divided into two groups: group I with signs of diabetic non-proliferative retinopathy (NPDR), and group II with signs of diabetic proliferative retinopathy (P/PDR).

The behaviour of bone mineral density and bone metabolism index in young and menopausal women with the consideration of body mass index.

Background: The bone fractures in patients with osteopeny have broaden the risk factors by body mass index (BMI) and bone metabolism assessment defined with bone scintigraphy. The aim of the study was to evaluate the behavior of bone mineral density (BMD) and bone metabolism index (IBM) in both young women and those more than 50 years old, as associated with BMI.

Material/methods: BMD and IBM were assessed in young women (group 1; n=66) and those after 50 (group II; n=67) with the allowance for BMI (< or =25 kg/cm(2) and >25 kg/cm(2)).