Modifications in the handling in vitro of 125I-labelled keyhole limpet haemocyanin by peritoneal macrophages from mice pretreated with the adjuvant Corynebacterium parvum.

Peritoneal macrophages were isolated from C. parvum-pretreated (CP) and normal CBAT6T6 mice and their in vitro handling of 125I-labelled Keyhole limpet haemocyanin (125I-labelled KLH) studied in relation to the humoral anti-KLH responses induced in corresponding animals. CP pretreatment exerted an adjuvant effect on the production of anti-KLH antibodies, both IgM and IgG, which was also demonstrable with a normally subimmunogenic dose of antigen.

The intracellular distribution of cathepsins and other acid hydrolases in mouse peritoneal macrophages.

Acid hydrolases, among them cathepsins A, B, C, and D, and their intracellular distribution have been studied in mouse peritoneal macrophages, a model commonly used for the study of mononuclear phagocyte functions in host-parasite relationships and in the immune response. Thioglycollate stimulated peritoneal macrophages showed high specific activities of acid hydrolases in compatison to liver and spleen. Fractionation of these macrophages by differential and isopycnic sucrose density centrifugation showed heterogeneous enyzme distribution patterns which were different for the various hydrolases studied.

The isolation and characterization of lysosomal particles from myxamoebae of the cellular slime mould Dictyostelium discoideum.

1. The myxamoebae of the cellular slime mould Dictyostelium discoideum possess several typically lysosomal enzyme activities. 2.

Macrophage cultures: an extracellular esterase.

An enzyme having esterase activity accumulates in the culture media of mouse peritoneal mononuclear leukocytes during differentiation of the cells into macrophages. It has a pH optimum of 6.5 and shows aryl esterase characteristics.