Further Evidence That Female (Coleoptera: Cerambycidae) Utilizes Photo-Degradation to Produce Volatiles That Are Attractive to Adult Males.

The Asian longhorned beetle, (Coleoptera: Cerambycidae: Lamiinae), is a serious pest of over 43 species of hardwood trees in North America, China and Europe. The development of an effective lure and trap for monitoring has been hindered by the fact that mate finding involves a rather complex series of behaviors and responses to several chemical (and visual), cues. Adults (female-biased) locate a tree via host kairomones.

Impervious cover change as an indicator of environmental equity.

Pattern-focused environmental equity research has been underpinned by high-resolution remotely sensed data to uncover spatial relationships between environmental amenities (e.g., urban tree cover) and socio-economic status (SES).

Safety and efficacy of an allogeneic adipose-derived mesenchymal stem cell preparation in the treatment of knee osteoarthritis: A Phase I/IIa randomised controlled trial.

Objectives: To assess the safety and efficacy of an allogeneic adipose-derived mesenchymal stem cell preparation (MAG200) in the treatment of knee osteoarthritis over 12 months.

Design: A single-centre, double-blind, ascending dose, randomised controlled trial. 40 participants with moderate knee osteoarthritis were randomised to receive a single intra-articular injection of MAG200 (dose cohorts:10, 20, 50, 100 ​× ​10 ​cells) or placebo.

Swift regulation of nicotinic acetylcholine receptors (nAChRs) and glutathione S-transferase (GST) enables the rapid detoxification of thiacloprid in pine sawyer beetles.

Thiacloprid, a neonicotinoid insecticide, has become one of the major control agents for the pine sawyer beetle, Monochamus alternatus Hope, however, the mechanism of detoxification is unknown. We demonstrate that glutathione S-transferases (GSTs) and nicotinic acetylcholine receptors (nAChRs) are involved in the rapid detoxification of thiacloprid in M. alternatus larvae.

HcGr76 responds to fructose and chlorogenic acid and is involved in regulation of peptide expression in the midgut of Hyphantria cunea larvae.

Background: Sensing dietary components in the gut is important to ensure an appropriate hormonal response and metabolic regulation after food intake. The fall webworm, Hyphantria cunea, is a major invasive pest in China and has led to significant economic losses and ecosystem disruption. The larvae's broad host range and voracious appetite for leaves make H.

Revisiting the Landscape Mosaic model.

The landscape mosaic model quantifies and maps the spatial juxtaposition of different land uses. It provides a landscape perspective of anthropic threats posed by agriculture and urban development, and the spatial-temporal shifting of the landscape mosaic indicates landscapes where anthropic intensity has changed. We use the U.

Genomic identification and evolutionary analysis of chemosensory receptor gene families in two Phthorimaea pest species: insights into chemical ecology and host adaptation.

Background: Insects rely on sophisticated sensitive chemosensory systems to sense their complex chemical environment. This sensory process involves a combination of odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) in the chemosensory system. This study focused on the identification and characterization of these three types of chemosensory receptor genes in two closely related Phthorimaea pest species, Phthorimaea operculella (potato tuber moth) and Phthorimaea absoluta (tomato leaf miner).

Differential Dominance of Ecological Processes Shapes the Longhorn Beetle Community in Tropical Rainforests and Temperate Forests of Southwest China.

(1) Background: Understanding the relationship between community assembly and species coexistence is key to understanding ecosystem diversity. Despite the importance of wood-boring longhorn beetles (Cerambycidae) in forests, factors affecting their population dynamics, species richness, and ecological interactions remain underexplored. (2) Methods: We surveyed cerambycid beetles and plants within five plots each across three transects in tropical rainforests and temperate forests of Yunnan, China, known for its rich biodiversity and varied elevation gradients.

Effect of a coaching intervention to enhance physical activity and prevent falls in community-dwelling people aged 60+ years: a cluster randomised controlled trial.

Objectives: To evaluate the effect of a coaching intervention compared with control on physical activity and falls rate at 12 months in community-dwelling people aged 60+ years.

Design: Cluster randomised controlled trial.

Setting: Community-dwelling older people.

Electrophysiological and Behavioral Responses of Hope to Volatiles from Bunge.

Following infestation by phytophagous insects, changes in the composition and relative proportion of volatile components emitted by plants may be observed. Some phytophagous insects can accurately identify these compounds to locate suitable host plants. We investigated whether herbivore-induced plant volatiles (HIPVs) generated by herbivory on Bunge (Sapindales: Aceraceae) might be semiochemicals for the host location of Hope (Coleoptera: Cerambycidae).

Disruption of nontuberculous mycobacteria biofilms induces a highly vulnerable to antibiotic killing phenotype.

Objectives: Structural or mucus hypersecretory pulmonary diseases such as cystic fibrosis (CF), wherein viscous mucus accumulates and clearance functions are impaired, predispose people to lung infection by inhaled bacteria that form biofilm aggregates. Nontuberculous mycobacteria (NTM), primarily and are the growing cause of these lung infections and are extremely challenging to treat due to antibiotic recalcitrance. Better therapeutic approaches are urgently needed.

Emerald Ash Borer Management and Research: Decades of Damage and Still Expanding.

Since the discovery of the ash tree ( spp.) killer emerald ash borer (EAB; ) in the United States in 2002 and Moscow, Russia in 2003, substantial detection and management efforts have been applied to contain and monitor its spread and mitigate impacts. Despite these efforts, the pest continues to spread within North America.

Optogenetics for controlling seizure circuits for translational approaches.

The advent of optogenetic tools has had a profound impact on modern neuroscience research, revolutionizing our understanding of the brain. These tools offer a remarkable ability to precisely manipulate specific groups of neurons with an unprecedented level of temporal precision, on the order of milliseconds. This breakthrough has significantly advanced our knowledge of various physiological and pathophysiological processes in the brain.

Development of a novel definitive scoring system for an enteral feed-only model of necrotizing enterocolitis in piglets.

Introduction: Necrotizing enterocolitis (NEC) is a complex inflammatory disorder of the human intestine that most often occurs in premature newborns. Animal models of NEC typically use mice or rats; however, pigs have emerged as a viable alternative given their similar size, intestinal development, and physiology compared to humans. While most piglet NEC models initially administer total parenteral nutrition prior to enteral feeds, here we describe an enteral-feed only piglet model of NEC that recapitulates the microbiome abnormalities present in neonates that develop NEC and introduce a novel multifactorial definitive NEC (D-NEC) scoring system to assess disease severity.

Combinatorial gene therapy for epilepsy: Gene sequence positioning and AAV serotype influence expression and inhibitory effect on seizures.

Gene therapy with AAV vectors carrying genes for neuropeptide Y and its receptor Y2 has been shown to inhibit seizures in multiple animal models of epilepsy. It is however unknown how the AAV serotype or the sequence order of these two transgenes in the expression cassette affects the actual parenchymal gene expression levels and the seizure-suppressant efficacy. To address these questions, we compared three viral vector serotypes (AAV1, AAV2 and AAV8) and two transgene sequence orders (NPY-IRES-Y2 and Y2-IRES-NPY) in a rat model of acutely induced seizures.

Factors Guiding the Orientation of Nymphal Spotted Lanternfly, .

A mark-release-recapture experiment was conducted to evaluate the orientation of spotted lanternfly (SLF) White (Hemiptera: Fulgoridae) nymphs when released equidistant between two trees. The experiment was repeated weekly for eight weeks in a heavily infested area with mature tree-of-heaven (Mill.) Swingle (Sapindales: Simaroubaceae) planted in rows as ornamental street trees in Beijing, China.

Modeling future land cover and water quality change in Minneapolis, MN, USA to support drinking water source protection decisions.

Continued alteration of the nitrogen cycle exposes receiving waters to elevated nitrogen concentrations and forces drinking water treatment services to plan for such increases in the future. We developed four 2011-2050 land cover change scenarios and modeled the impact of projected land cover change on influent water quality to support long-term planning for the Minneapolis Water Treatment Distribution Service (MWTDS) using Soil Water and Assessment Tool. Projected land cover changes based on relatively unconstrained economic growth led to substantial increases in total nitrogen (TN) loads and modest increases in total phosphorus (TP) loads in spring.

A mesh microelectrode array for non-invasive electrophysiology within neural organoids.

Organoids are emerging in vitro models of human physiology. Neural models require the evaluation of functional activity of single cells and networks, which is commonly measured by microelectrode arrays. The characteristics of organoids clash with existing in vitro or in vivo microelectrode arrays.

Reduced epilepsy development in synapsin 2 knockout mice with autistic behavior following early systemic treatment with interleukin-6 receptor antibody.

Background: Individuals with autism spectrum disorder (ASD) have an increased risk of developing epilepsy. Both ASD and epilepsy have been associated with increased levels of immune factors in the blood, including the proinflammatory cytokine interleukin 6 (IL-6). Mice lacking the synapsin 2 gene (Syn2 KO) exhibit ASD-like behavior and develop epileptic seizures.

Thematic accuracy assessment of the NLCD 2019 land cover for the conterminous United States.

The National Land Cover Database (NLCD), a product suite produced through the MultiResolution Land Characteristics (MRLC) consortium, is an operational land cover monitoring program. Starting from a base year of 2001, NLCD releases a land cover database every 2-3-years. The recent release of NLCD2019 extends the database to 18 years.

Where forest may not return in the western United States.

Droughts that are hotter, more frequent, and last longer; pest outbreaks that are more extensive and more common; and fires that are more frequent, more extensive, and perhaps more severe have raised concern that forests in the western United States may not return once disturbed by one or more of these agents. Numerous field-based studies have been undertaken to better understand forest response to these changing disturbance regimes. Meta-analyses of these studies provide broad guidelines on the biotic and abiotic factors that hinder forest recovery, but study-to-study differences in methods and objectives do not support estimation of the total extent of potentially impaired forest succession.

Cell-specific switch for epileptiform activity: critical role of interneurons in the mouse subicular network.

During epileptic seizures, neuronal network activity is hyper synchronized whereby GABAergic parvalbumin-interneurons may have a key role. Previous studies have mostly utilized 4-aminopyridine to induce epileptiform discharges in brain slices from healthy animals. However, it is not clear if the seizure-triggering ability of parvalbumin-interneurons also holds true without the use of external convulsive agents.

Associated bacteria of a pine sawyer beetle confer resistance to entomopathogenic fungi via fungal growth inhibition.

Background: The entomopathogenic Beauveria bassiana is a popular fungus used to control the Japanese pine sawyer, Monochamus alternatus Hope, the key vector of pine wood nematode (Bursaphelenchus xylophilus) that is the causal agent of pine wilt disease, resulting in devastating losses of pines in China and Portugal. However, recent studies have demonstrated that some insect-associated bacteria might decrease fungal toxicity and further undermine its biological control efficacy against M. alternatus.

Low-dose mesenchymal stem cell therapy for discogenic pain: safety and efficacy results from a 1-year feasibility study.

Aim: To evaluate safety and efficacy of low dose autologous adipose-derived mesenchymal stem cells (ADMSCs) for treatment of disc degeneration resulting in low back pain (LBP).

Methods: Nine participants with chronic LBP originating from single-level lumbar disc disease underwent intradiscal injection of 10 million ADMSCs with optional repetition after 6 months.

Results: No unexpected or serious adverse events were recorded.