Cryo-electron tomography of eel sperm flagella reveals a molecular "minimum system" for motile cilia.

Cilia and flagella play a crucial role in the development and function of eukaryotes. The activity of thousands of dyneins is precisely regulated to generate flagellar motility. The complex proteome (600+ proteins) and architecture of the structural core of flagella, the axoneme, have made it challenging to dissect the functions of the different complexes, like the regulatory machinery.

Analyzing Rho GTPase-Dependent Processes During Cell Polarization in Brown Algae.

Zygotes of the fucoid brown algae are useful models for investigating the molecular and cellular mechanisms of cell polarization. These organisms are abundant in the marine intertidal zone, where they grow firmly anchored to rocks. In response to environmental cues like sunlight, zygotes generate asymmetries within the cell that ultimately establish an axis of growth.

Rac1 signaling in the establishment of the fucoid algal body plan.

Fucoid zygotes use environmental vectors, including sunlight, to initiate a growth axis a few hours after fertilization. The first division is then transversely oriented by the growth axis, producing daughter cells of distinct fates. The tip growing rhizoid cell gives rise to the holdfast, anchoring the alga to the intertidal substratum, while the opposite thallus cell mainly generates the photosynthetic and reproductive stipe and fronds.

Ultrastructural and structural characterization of zygotes and embryos during development in Sargassum cymosum (Phaeophyceae, Fucales).

This study investigates the pattern and performance of cellular structures during the early development of zygotes and embryos of Sargassum cymosum. The early development S. cymosum germlings has already been characterized and compared with the pattern of development established for all fucoid algae, in which the zygote remains attached to the receptacle by mucilage during the establishment of polarity and early cell division.

RAC1 regulates actin arrays during polarity establishment in the brown alga, Silvetia compressa.

Multicellular development has evolved independently on numerous occasions and there is great interest in the developmental mechanisms utilized by each of the divergent lineages. Fucoid algae, in the stramenopile lineage (distinct from metazoans, fungi and green plants) have long been used as a model for early development based on unique life cycle characteristics. The initially symmetric fucoid zygote generates a developmental axis that determines not only the site of growth, but also the orientation of the first cell division, whose products have distinct developmental fates.

Polychlorinated biphenyls disrupt cell division and tip growth in two species of fucoid algae.

Environmental contaminants, including poly-chlorinated biphenyls (PCBs), are enriched in coastal sediments, and despite a 1977 moratorium by the United States Environmental Protection Agency on the production of PCBs, levels remain high, more so near former industrial plants. The effects of these contaminants on sessile species in the intertidal zone, particularly nonanimal species such as the ubiquitous fucoid brown algae, are not well known. We investigated the developmental effects of chronic PCB treatment beginning at fertilization on two species of marine rockweed, Fucus vesiculosus Linnaeus and Silvetia compressa (J.

Signaling mechanisms in the establishment of plant and fucoid algal polarity.

The establishment of polarity is a fundamental property of most cells. In tip-growing plant and in fucoid algal cells, polarization specifies a growth pole, the center of localized secretion of new plasma membrane and cell wall material, generating a protrusion with a dome-shaped apex. Although much progress has been made concerning the cellular machinery required to execute tip growth, less is known regarding the signaling mechanisms involved in selecting the growth site and regulating vectorial cell division and expansion.

Rac1 function during fucoid development.

Morphogenesis in fucoid algae begins with adhesive secretion and rhizoid germination, developmental events that secure the alga within the intertidal zone. The importance of the actin cytoskeleton during these processes has been well established; but in general, little is known about actin regulation within the stramenopile lineage. Based on conserved strategies for regulation of actin in other lineages, co-localization of the Arp2/3 complex with actin structures that are essential for rhizoid formation may implicate members of the Rho family of small GTPases in the signaling pathway(s) regulating actin polymerization during fucoid development.

The Rac1 inhibitor, NSC23766, depolarizes adhesive secretion, endomembrane cycling, and tip growth in the fucoid alga, Silvetia compressa.

Proper cell morphogenesis is dependent on the establishment and expression of cellular polarity. In the fucoid zygote, cell shape is critical for establishing the developmental pattern of the adult, and is achieved by guiding insertion of new membrane and wall to the rhizoid tip. Selection and growth of the appropriate tip site are accompanied by formation of dynamic actin arrays associated with the actin-nucleating Arp2/3 complex.

Polarization of the endomembrane system is an early event in fucoid zygote development.

Background: Fucoid zygotes are excellent experimental organisms for investigating mechanisms that establish cell polarity and determine the site of tip growth. A common feature of polarity establishment is targeting endocytosis and exocytosis (secretion) to localized cortical domains. We have investigated the spatiotemporal development of endomembrane asymmetry in photopolarizing zygotes, and examined the underlying cellular physiology.

The Arp2/3 complex nucleates actin arrays during zygote polarity establishment and growth.

Previous work has demonstrated that dynamic actin arrays are important for axis establishment and polar growth in the fucoid zygote, Silvetia compressa. Transitions between these arrays are mediated by depolymerization of an existing array and polymerization of a new array. To begin to understand how polymerization of new arrays might be regulated, we investigated the role of the highly conserved, actin-nucleating, Actin-related protein 2/3 (Arp2/3) complex.