Transcriptome alterations in HepG2 cells induced by shRNA knockdown and overexpression of gene.

Transmembrane 2 () gene inhibits chronic hepatitis-B virus (HBV) infection, while the underlying molecular mechanisms remain unknown. Transcriptome alterations in HepG2 cells following overexpression or silencing by shRNA were analyzed by next-generation sequencing. Both overexpression and knockdown of the gene caused wide-spread changes in gene expression in HepG2 cells.