Sonolysis promotes indirect Co-C bond cleavage of alkylcob(III)alamin bioconjugates.

Sonolysis of aqueous solutions produces H. and HO. that lead to Co-C bond cleavage in methylcob-(III)alamin (CH3-CblIII) and 2-[4-[4'-[bis(2-chloroethyl)amino]phenyl]butyroxy]ethylcob (III)alamin (Chl-HE-CblIII).

[Experiences with microcomputer based registration of esophago-gastro-duodenoscopy].

The development of and the experience with a microcomputer based register of endoscopy is described. The paper work was reduced from nine forms to one. The register provides the information necessary for budgeting the resources for staff and new equipment.

Antiviral antibodies in the sera of homosexual men: correlation with their lifestyle and drug usage.

Healthy homosexual men between the ages of 21 and 65 years, from the Washington, DC (n = 162), and New York City (n = 89) areas, were studied for antibodies in the serum against cytomegalovirus (CMV), herpes simplex virus (HSV) types 1 and 2, and Epstein Barr virus (EBV) viral capsid antigen (VCA). CMV-specific antibodies were assayed by enzyme-linked immunosorbent assay (ELISA), anti-HSV-1 and -2 antibodies were measured by indirect hemagglutination (IHA), and antibodies to EBV VCA were measured by the immunofluorescence assay. Antibodies to human T lymphotrophic virus III (HTLV-III) were detected by ELISA and Western blot procedures.

Dysplasia in gastric mucosa: prognostic significance.

Forty-seven patients having dysplasia in biopsies from the gastric mucosa were subjected to re-investigation. Twenty-three patients were alive at follow-up and 14 underwent gastroscopy with removal of random biopsies. The median duration of observation was 39 months, range 10-63 months.

Survival of herpes simplex virus in water specimens collected from hot tubs in spa facilities and on plastic surfaces.

Several health spas were closed temporarily because of possible nonvenereal spread of herpes simplex virus (HSV) in spa water at these facilities. We collected water specimens from two health spas and studied them for (1) the presence of HSV; (2) bromine (Br2), chlorine (Cl2), and pH levels; and (3) the ability of HSV to survive in water. No HSV could be isolated from the spa water specimens.

Rapid washing of filter paper discs in a solid-phase radioimmunoassay with a constant flow washing device.

A machine has been developed for the rapid washing of the cellulose filter paper discs that are used in a number of radioimmunoassays. The machine is simple in design, easy to use, and is capable of washing 96 filter paper discs simultaneously. The efficiency of the machine is demonstrated by a RAST assay for measuring IgE antibodies to the venom.

Acute effects of dietary protein on calcium metabolism in patients with osteoporosis.

Five untreated osteoporotic patients were studied in a metabolic unit for 30 days while receiving control (0.8 gm/kg) and high (2.0 gm/kg) protein diets of purified proteins.