Epithelial and mesenchymal prostate cancer cell population dynamics on a complex drug landscape.

We have improved our microfluidic cell culture device that generates an landscape of stress heterogeneity. We now can do continuous observations of different cancer cell lines and carry out downstream analysis of cell phenotype as a function of position on the stress landscape. We use this technology to probe adaption and evolution dynamics in prostate cancer cell metapopulations under a stress landscape of a chemotherapeutic drug (docetaxel).