Various type immobilizations of Isocitrate dehydrogenases enzyme on hyaluronic acid modified magnetic nanoparticles as stable biocatalysts.

Magnetic nanoparticles (MNPs) were modified by hyaluronic acid (HA). After the process of functionalization, two different strategies have been used to immobilize isocitrate dehydrogenases (IDH) on MNPs. In the first strategy, cross-linked enzyme aggregates were prepared.

The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis.

Background: The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis.

Methods: We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis.

The novel multi cross-linked enzyme aggregates of protease, lipase, and catalase production from the sunflower seeds, characterization and application.

The cross-linked enzyme aggregates (CLEAs) have numerous economic advantages in the industrial bio catalysis. In the present study, the multi CLEAs containing protease, catalase, and lipase from the sunflower seeds using starch as a cofeeder as well as bovine serum albumin (BSA) are designed and prepared successfully. After optimization, multi CLEAs of enzyme have been prepared with ammonium sulfate (55% w/v), glutaraldehyde (100 mM), and 8 mg/mL of starch or 20 mg/mL of BSA.

Lipase-based on starch material as a development matrix with magnetite cross-linked enzyme aggregates and its application.

The FeO magnetic nanoparticles were prepared by precipitating ferrous ion (Fe) and ferric ion (Fe) in alkaline solution. The FeO magnetic nanoparticles were modified by tannic acid. After functionalization process, two methods were used to immobilize Lipase on FeO magnetic nanoparticles.

Evaluation of different saccharides and chitin as eco-friendly additive to improve the magnetic cross-linked enzyme aggregates (CLEAs) activities.

The cross-linked enzyme aggregates (CLEAs) involves formation of a number of covalent bonds between enzyme and the matrix using glutaraldehyde. In general, amino groups of lysine, sulfhydryl groups of cysteine, phenolic OH groups of tyrosine, or imidazol group of histidine are used for enzyme binding under mild conditions. The main advantage of this method is its simplicity, economic advantages in the industrial bio catalysis.

Genetic polymorphisms of human transcription factor-7 like 2 (TCF7L2), β-defensin (DEFB1) and CD14 genes in nephrolithiasis patients.

Background: It is assumed that genetic factors play crucial role in nephrolithiasis. The present study was conducted to explore the role of Human Transcription Factor-7 like-2 (TCF7L2) β-defensin (DEFB1) and CD14 gene polymorphism in development and progression of nephrolithiasis.

Methods: The genotypes of TCF7L2, DEFB1 and CD14 polymorphism were determined in 240 nephrolithiasis patients and 240 healthy controls by restriction digestion method of PCR.

Characterization and immobilization of protease and lipase on chitin-starch material as a novel matrix.

Protease and lipase were purified from sunflower seeds by frequent purification steps with molecular weights of 72.90 kDa and 27.50 kDa, respectively.

Determination of alpha-2-MRAP gene polymorphisms in nephrolithiasis patients.

Background: The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients.

Methods: PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism.

Association of MMP-9 gene polymorphisms with nephrolithiasis patients.

Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease (CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular matrix which correlate with the pathogenesis of atherosclerosis. The current study was designed to analyze the association of (R279Q, C1562T) polymorphism of MMP-9 with nephrolithiasis patients.

Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

Objective: Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage.

Methods: Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study.

Effects of Acute Lymphoblastic Leukemia on Ceruloplasmin Oxidase, Copper and Several Markers of Oxidative Damage, in Children.

Background: Acute leukaemia is characterized by fast growth of abnormal clones of haemopoietic precursor cells inside bone marrow leading to undue accumulation in the bone marrow. Acute lymphoblastic leukemia (ALL) is the most common form of childhood cancer.

Materials And Methods: The study concerned 50 children diagnosed with ALL (mean age, 8.

CD26: A Prognostic Marker of Acute Lymphoblastic Leukemia in Children in the Post Remission Induction Phase.

Background: ALL is an irredeemable disease due to the resistance to treatment. There are several influences which are involved in such resistance to chemotherapy, including oxidative stress as a result of the generation of reactive oxygen species (ROS) and presence of hypodiploid cells. Cluster of differentiation 26 (CD26), also known as dipeptidyl peptidase-4, is a 110 kDa, multifunctional, membrane-bound glycoprotein.

Correlation of inhibin and several antioxidants in children with acute lymphoblastic leukemia.

Background: Acute lymphoblastic leukemia (ALL) is most common in childhood. Inhibin (a non-steroidal glycoprotein hormone of gonadal origin) can be used as marker of fertility. The current study was conducted to evaluate inhibin levels in ALL patients and to estimate its correlation with some antioxidants in these in comparison with control subjects.