Development of a method for the determination of sedatives in bovine and porcine urine and kidneys by liquid chromatography-tandem mass spectrometry.

Introduction: Sedatives have been used for a long time as animal tranquillisers to prevent stress and weight loss during their transportation. The proper determination of these substances in food of animal origin is essential for consumer safety.

Material And Methods: A 1 g portion of pig or cow urine or homogenised kidney was mixed with acetonitrile, sodium chloride was added, and the solution was further mixed and then centrifuged.