Comparative analysis of primer sets for the assessment of clonality in feline lymphomas.

Introduction: Lymphomas are among the most important and common malignant tumors in cats. Differentiating lymphomas from reactive lymphoid proliferations can be challenging, so additional tools such as clonality assessment by PCR are important in diagnosis finding. Several PCR assays have been developed to assess clonality in feline lymphomas.

The LASSIE MPS panel: Predicting externally visible traits in dogs for forensic purposes.

Predicting the outward appearance of dogs via their DNA, also known as Canine DNA Phenotyping, is a young, emerging field of research in forensic genetics. The few previous studies published in this respect were restricted to the consecutive analysis of single DNA markers, a process that is time- and sample-consuming and therefore not a viable option for limited forensic specimens. Here, we report on the development and evaluation of a Massively Parallel Sequencing (MPS) based molecular genetic assay, the LASSIE MPS Panel.

Towards Forensic DNA Phenotyping for Predicting Visible Traits in Dogs.

The popularity of dogs as human companions explains why these pets regularly come into focus in forensic cases such as bite attacks or accidents. Canine evidence, e.g.

Lack of Bcl-2 expression in feline follicular lymphomas.

Bcl-2, an anti-apoptotic protein, is commonly overexpressed in follicular lymphomas in humans. This is usually the result of a chromosomal translocation that transposes the gene into the immunoglobulin gene locus. The immunohistochemical assessment of this overexpression can be used as a tool for the differentiation of follicular lymphoma and follicular hyperplasia.

Forensic characterization and statistical considerations of the CaDNAP 13-STR panel in 1,184 domestic dogs from Germany, Austria, and Switzerland.

Crime scene samples originating from domestic dogs such as hair, blood, or saliva can be probative as possible transfer evidence in human crime and in dog attack cases. In the majority of such cases canine DNA identification using short tandem repeat (STR) analysis is the method of choice, which demands, among others, a systematic survey of allele frequency data in the relevant dog populations. A set of 13 highly polymorphic canine STR markers was used to analyze samples of 1,184 dogs (including 967 purebred dogs) from the so-called DACH countries (Germany, Austria, Switzerland).

Dog breed affiliation with a forensically validated canine STR set.

We tested a panel of 13 highly polymorphic canine short tandem repeat (STR) markers for dog breed assignment using 392 dog samples from the 23 most popular breeds in Austria, Germany, and Switzerland. This STR panel had originally been selected for canine identification. The dog breeds sampled in this study featured a population frequency ≥1% and accounted for nearly 57% of the entire pedigree dog population in these three countries.

High resolution melting analysis (HRM) for the assessment of clonality in feline B-cell lymphomas.

Unlabelled: Analysis of clonality is gaining importance in diagnosing lymphomas in veterinary medicine. Usually, PCR for the analysis of antigen receptor rearrangement (PARR) is followed by electrophoretic separation of the PCR products. Aim of this study was to test the feasibility of HRM for the assessment of clonality in B-cell lymphomas of cats.

Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.

Feline large granular lymphocyte lymphomas are rare but very aggressive tumors with a poor prognosis. In this study, a cell line from an abdominal effusion of a cat with large granular lymphoma was characterized. Immunophenotype staining was positive for CD3 and CD45R, and negative for CD4, CD8, CD56, CD79α, BLA.

Molecular characterization of the feline T-cell receptor γ alternate reading frame protein (TARP) ortholog.

T-cell receptor γ alternate reading frame protein (TARP) is expressed by human prostate epithelial, prostate cancer, and mammary cancer cells, but is not found in normal mammary tissue. To date, this protein has only been described in humans. Additionally, no animal model has been established to investigate the potential merits of TARP as tumor marker or a target for adoptive tumor immunotherapy.

Feline T-Cell Receptor gamma V- and J-Region Sequences Retrieved from the Trace Archive and from Transcriptome Analysis of Cats.

The variable domains of antigen receptors are very diverse and assembled in a modular system from a number of V-, D-, and J-region genes. Here we describe additional variants of V- and J-region genes of the feline T-cell receptor gamma (TRG) as well as the corresponding RSSs retrieved from Trace Archive of feline genomic sequences. Additionally, an unusually recombined TRGV-domain containing a partial inverted repeat of the included J-region and a short interspersed element of the CAN-SINE family located within the feline T-cell receptor gamma locus are also described.

[Disease monitoring in European mouflon (Ovis gmelini musimon) populations by clinical blood tests--aspects of epidemiology and treatment control of claw diseases].

European mouflon are in the focus of research since they were brought from the Tyrrhenic islands to the European mainland a hundred years ago. From the beginning many populations on European mainland suffer from different claw diseases which are unknown in their original habitats. Foot rot, the ovine purulent laminitis, whose existence im wild ruminants was negotiated some years before, furthermore claw alterations caused by primary or secondary lack of trace elements similar to the copper deficiency syndrome of the boreal deer species moose and reindeer and finally horn hyperplasia with a genetic background are found as main claw diseases in Central Europe.